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| Name | 2-Propanol for HPLC |
| Formula | C3H8O |
| CAS Number | 67-63-0 |
| Molecular Weight | 60.1 g/mol |
| Purity | ≥ 99.9% |
| Appearance | Clear liquid |
| Boiling Point | 82.5°C |
| Melting Point | -88.5°C |
| Solubility | Miscible in water |
| Storage Conditions | Store in a cool, dry, well-ventilated area away from incompatible substances |
| Applications | Used as a solvent in high-performance liquid chromatography (HPLC) |
| Hazards | Highly flammable liquid and vapor |
FAQ
What is 2-Propanol and how is it used in HPLC analysis?
2-Propanol, also known as isopropanol or IPA, is a common solvent used in various analytical techniques, including in High Performance Liquid Chromatography (HPLC). In HPLC analysis, 2-Propanol is often used as a mobile phase solvent due to its ability to interact with different compounds and carry them through the chromatographic column for separation.
What are the advantages of using 2-Propanol in HPLC analysis?
One of the main advantages of using 2-Propanol in HPLC analysis is its versatility as a solvent. It has a low viscosity, which allows for efficient flow through the chromatographic system, leading to faster analysis times. Additionally, 2-Propanol has a relatively low UV cut-off, making it suitable for use with UV detectors commonly used in HPLC. Its compatibility with a wide range of compounds also makes it a popular choice for method development in HPLC analysis.
How does 2-Propanol compare to other solvents commonly used in HPLC analysis?
2-Propanol has several advantages over other solvents commonly used in HPLC analysis. For example, it has a lower UV cut-off compared to methanol and acetonitrile, allowing for more accurate detection of compounds with lower absorbance at higher wavelengths. Additionally, 2-Propanol is less toxic than methanol and acetonitrile, making it a safer choice for laboratory personnel. Its lower cost and availability also make it a cost-effective option for routine HPLC analysis.
Are there any considerations to keep in mind when using 2-Propanol in HPLC analysis?
While 2-Propanol is a versatile solvent for HPLC analysis, there are some considerations to keep in mind when using it in chromatographic separations. Due to its lower elution strength compared to other solvents like acetonitrile, method development may require optimization of gradient profiles and column conditions. Additionally, 2-Propanol can interact differently with stationary phases, so it is important to ensure compatibility with the specific column being used in the HPLC system. Regular maintenance and monitoring of system performance are also essential to minimize any potential issues with 2-Propanol in HPLC analysis.
In conclusion, 2-Propanol is a versatile solvent that offers several advantages for HPLC analysis, including its low viscosity, compatibility with a wide range of compounds, and cost-effectiveness. By considering its unique properties and optimizing method conditions, 2-Propanol can be effectively used in chromatographic separations to achieve accurate and reliable results.
2-Propanol, also known as isopropanol or IPA, is a common solvent used in various analytical techniques, including in High Performance Liquid Chromatography (HPLC). In HPLC analysis, 2-Propanol is often used as a mobile phase solvent due to its ability to interact with different compounds and carry them through the chromatographic column for separation.
What are the advantages of using 2-Propanol in HPLC analysis?
One of the main advantages of using 2-Propanol in HPLC analysis is its versatility as a solvent. It has a low viscosity, which allows for efficient flow through the chromatographic system, leading to faster analysis times. Additionally, 2-Propanol has a relatively low UV cut-off, making it suitable for use with UV detectors commonly used in HPLC. Its compatibility with a wide range of compounds also makes it a popular choice for method development in HPLC analysis.
How does 2-Propanol compare to other solvents commonly used in HPLC analysis?
2-Propanol has several advantages over other solvents commonly used in HPLC analysis. For example, it has a lower UV cut-off compared to methanol and acetonitrile, allowing for more accurate detection of compounds with lower absorbance at higher wavelengths. Additionally, 2-Propanol is less toxic than methanol and acetonitrile, making it a safer choice for laboratory personnel. Its lower cost and availability also make it a cost-effective option for routine HPLC analysis.
Are there any considerations to keep in mind when using 2-Propanol in HPLC analysis?
While 2-Propanol is a versatile solvent for HPLC analysis, there are some considerations to keep in mind when using it in chromatographic separations. Due to its lower elution strength compared to other solvents like acetonitrile, method development may require optimization of gradient profiles and column conditions. Additionally, 2-Propanol can interact differently with stationary phases, so it is important to ensure compatibility with the specific column being used in the HPLC system. Regular maintenance and monitoring of system performance are also essential to minimize any potential issues with 2-Propanol in HPLC analysis.
In conclusion, 2-Propanol is a versatile solvent that offers several advantages for HPLC analysis, including its low viscosity, compatibility with a wide range of compounds, and cost-effectiveness. By considering its unique properties and optimizing method conditions, 2-Propanol can be effectively used in chromatographic separations to achieve accurate and reliable results.
