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| Product Name | Acetonitrile for LC-MS |
| Brand | Sigma-Aldrich |
| Purity | ≥99.9% |
| Grade | LC-MS |
| CAS Number | 75-05-8 |
| Molecular Formula | C2H3N |
| Molecular Weight | 41.05 g/mol |
| Appearance | Colorless liquid |
| Boiling Point | 81.6°C |
| Density | 0.786 g/mL at 25°C |
| Solubility | Miscible with water, ethanol, ether |
| Storage Temperature | 2-8°C |
| Packaging | Glass bottle |
| Shelf Life | 2 years |
| UN Number | 1648 |
| MSDS | Available upon request |
FAQ
What is acetonitrile and how is it used in LC-MS analysis?
Acetonitrile is a common solvent used in liquid chromatography-mass spectrometry (LC-MS) analysis. It is often used as a mobile phase in the chromatographic separation of compounds before ionization and detection in the mass spectrometer. Acetonitrile helps to elute compounds from the column efficiently and is compatible with a wide range of analytes.
What are the benefits of using acetonitrile for LC-MS analysis?
Acetonitrile has several advantages when used in LC-MS analysis. It has a low UV cut-off, which means that it does not interfere with the detection of compounds in the ultraviolet range. Acetonitrile also evaporates quickly, which is important for achieving good sensitivity in the mass spectrometer. Additionally, acetonitrile is compatible with a variety of stationary phases and analytical techniques, making it a versatile solvent for LC-MS analysis.
Are there any limitations or considerations when using acetonitrile in LC-MS analysis?
While acetonitrile is a widely used solvent in LC-MS analysis, there are some limitations and considerations to keep in mind. Acetonitrile can form adducts with certain compounds, leading to interference in the mass spectrum. It is also important to ensure the purity of the acetonitrile used in analysis, as impurities can affect the chromatographic separation and detection of compounds. Additionally, acetonitrile is a volatile and flammable solvent, so proper safety precautions should be taken when handling it in the laboratory.
How can I optimize the use of acetonitrile in my LC-MS analysis?
To optimize the use of acetonitrile in LC-MS analysis, it is important to consider factors such as the composition of the mobile phase, the flow rate of the solvent, and the type of column used. It is recommended to perform method development and optimization experiments to determine the best conditions for your specific analytes. Additionally, regular maintenance of the LC-MS system, including cleaning and calibrating the instrument, can help ensure accurate and reliable results when using acetonitrile as a solvent.
Are there any alternatives to acetonitrile for LC-MS analysis?
There are several alternatives to acetonitrile that can be used in LC-MS analysis, depending on the specific requirements of the analysis. Methanol is a common alternative solvent that is often used in LC-MS analysis, as it is less expensive than acetonitrile and can provide good chromatographic performance. Formic acid and ammonium formate are often added to the mobile phase to enhance sensitivity and improve ionization in the mass spectrometer. Ultimately, the choice of solvent and additives will depend on the specific analytes being studied and the desired analytical results.
Acetonitrile is a common solvent used in liquid chromatography-mass spectrometry (LC-MS) analysis. It is often used as a mobile phase in the chromatographic separation of compounds before ionization and detection in the mass spectrometer. Acetonitrile helps to elute compounds from the column efficiently and is compatible with a wide range of analytes.
What are the benefits of using acetonitrile for LC-MS analysis?
Acetonitrile has several advantages when used in LC-MS analysis. It has a low UV cut-off, which means that it does not interfere with the detection of compounds in the ultraviolet range. Acetonitrile also evaporates quickly, which is important for achieving good sensitivity in the mass spectrometer. Additionally, acetonitrile is compatible with a variety of stationary phases and analytical techniques, making it a versatile solvent for LC-MS analysis.
Are there any limitations or considerations when using acetonitrile in LC-MS analysis?
While acetonitrile is a widely used solvent in LC-MS analysis, there are some limitations and considerations to keep in mind. Acetonitrile can form adducts with certain compounds, leading to interference in the mass spectrum. It is also important to ensure the purity of the acetonitrile used in analysis, as impurities can affect the chromatographic separation and detection of compounds. Additionally, acetonitrile is a volatile and flammable solvent, so proper safety precautions should be taken when handling it in the laboratory.
How can I optimize the use of acetonitrile in my LC-MS analysis?
To optimize the use of acetonitrile in LC-MS analysis, it is important to consider factors such as the composition of the mobile phase, the flow rate of the solvent, and the type of column used. It is recommended to perform method development and optimization experiments to determine the best conditions for your specific analytes. Additionally, regular maintenance of the LC-MS system, including cleaning and calibrating the instrument, can help ensure accurate and reliable results when using acetonitrile as a solvent.
Are there any alternatives to acetonitrile for LC-MS analysis?
There are several alternatives to acetonitrile that can be used in LC-MS analysis, depending on the specific requirements of the analysis. Methanol is a common alternative solvent that is often used in LC-MS analysis, as it is less expensive than acetonitrile and can provide good chromatographic performance. Formic acid and ammonium formate are often added to the mobile phase to enhance sensitivity and improve ionization in the mass spectrometer. Ultimately, the choice of solvent and additives will depend on the specific analytes being studied and the desired analytical results.
