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| Product Name | Tetrabutylammonium di-Hydrogen Phosphate for HPLC |
| CAS Number | 83225-46-9 |
| Molecular Formula | C16H36NO4P |
| Molecular Weight | 341.43 g/mol |
| Physical State | Solid |
| Solubility | Soluble in water |
| Storage Temperature | Room temperature |
| Purity | ≥ 98% |
| Application | Used as an ion pairing reagent in high performance liquid chromatography (HPLC) |
| Appearance | White to off-white crystalline powder |
| Melting Point | 88-92°C |
| Boiling Point | N/A |
| Density | N/A |
| PH | 5-7 (10% solution in water) |
| Refractive Index | N/A |
| Flash Point | N/A |
| Handling | Avoid inhalation, ingestion, and contact with skin |
FAQ
What is Tetrabutylammonium di-Hydrogen Phosphate and how is it used in HPLC analysis?
Tetrabutylammonium di-Hydrogen Phosphate, commonly known as TBA Phosphate, is a strong ion pairing reagent commonly used in HPLC analysis. It is used to improve the resolution and retention of analytes, especially when analyzing highly polar compounds. TBA Phosphate forms a stable ion pair with analytes, helping to separate them more effectively during HPLC analysis.
What are the key benefits of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC applications?
Tetrabutylammonium di-Hydrogen Phosphate offers several key benefits when used in HPLC applications. Some of the main advantages include:
- Improved resolution and peak shape for polar compounds
- Enhanced retention of analytes, particularly for highly polar compounds
- Increased selectivity and sensitivity in HPLC analysis
- Reduced solvent consumption and improved chromatographic performance
- Compatibility with a wide range of analytical methods and HPLC systems.
How should Tetrabutylammonium di-Hydrogen Phosphate be optimized for use in HPLC analysis?
To optimize the use of Tetrabutylammonium di-Hydrogen Phosphate in HPLC analysis, it is important to consider several factors. These include:
- Determining the appropriate concentration of TBA Phosphate in the mobile phase
- Adjusting the pH of the mobile phase to ensure optimal ion pairing interactions
- Selecting the most suitable column for the separation of analytes
- Validating the method parameters for the desired analytical results
- Using proper sample preparation techniques to minimize interference and ensure accurate quantification.
Are there any considerations or precautions to keep in mind when using Tetrabutylammonium di-Hydrogen Phosphate in HPLC analysis?
While Tetrabutylammonium di-Hydrogen Phosphate can provide significant benefits in HPLC analysis, there are some considerations and precautions to keep in mind when using this reagent. These include:
- Ensuring proper storage and handling to prevent degradation or contamination of TBA Phosphate
- Using appropriate safety measures when working with TBA Phosphate, as it can be corrosive and toxic
- Validating the method performance and reproducibility before routine use in HPLC analysis
- Monitoring the chromatographic system for any changes or drift in performance
- Following regulatory guidelines and best practices for the use of Tetrabutylammonium di-Hydrogen Phosphate in analytical methods.
Tetrabutylammonium di-Hydrogen Phosphate, commonly known as TBA Phosphate, is a strong ion pairing reagent commonly used in HPLC analysis. It is used to improve the resolution and retention of analytes, especially when analyzing highly polar compounds. TBA Phosphate forms a stable ion pair with analytes, helping to separate them more effectively during HPLC analysis.
What are the key benefits of using Tetrabutylammonium di-Hydrogen Phosphate in HPLC applications?
Tetrabutylammonium di-Hydrogen Phosphate offers several key benefits when used in HPLC applications. Some of the main advantages include:
- Improved resolution and peak shape for polar compounds
- Enhanced retention of analytes, particularly for highly polar compounds
- Increased selectivity and sensitivity in HPLC analysis
- Reduced solvent consumption and improved chromatographic performance
- Compatibility with a wide range of analytical methods and HPLC systems.
How should Tetrabutylammonium di-Hydrogen Phosphate be optimized for use in HPLC analysis?
To optimize the use of Tetrabutylammonium di-Hydrogen Phosphate in HPLC analysis, it is important to consider several factors. These include:
- Determining the appropriate concentration of TBA Phosphate in the mobile phase
- Adjusting the pH of the mobile phase to ensure optimal ion pairing interactions
- Selecting the most suitable column for the separation of analytes
- Validating the method parameters for the desired analytical results
- Using proper sample preparation techniques to minimize interference and ensure accurate quantification.
Are there any considerations or precautions to keep in mind when using Tetrabutylammonium di-Hydrogen Phosphate in HPLC analysis?
While Tetrabutylammonium di-Hydrogen Phosphate can provide significant benefits in HPLC analysis, there are some considerations and precautions to keep in mind when using this reagent. These include:
- Ensuring proper storage and handling to prevent degradation or contamination of TBA Phosphate
- Using appropriate safety measures when working with TBA Phosphate, as it can be corrosive and toxic
- Validating the method performance and reproducibility before routine use in HPLC analysis
- Monitoring the chromatographic system for any changes or drift in performance
- Following regulatory guidelines and best practices for the use of Tetrabutylammonium di-Hydrogen Phosphate in analytical methods.
