2-Propanol for HPLC

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Name 2-Propanol for HPLC
Purity ≥99.9%
Appearance Clear colorless liquid
Synonyms Isopropanol, IPA, Propan-2-ol
Cas Number 67-63-0
Molecular Formula C3H8O
Molar Mass 60.10 g/mol
Boiling Point 82.6°C
Melting Point -89.5°C
Density 0.784 g/cm3
Solubility Soluble in water, ethanol, ether
FAQ
What is 2-Propanol used for in HPLC analysis?
2-Propanol, also known as isopropanol or isopropyl alcohol, is commonly used as a solvent in high performance liquid chromatography (HPLC) due to its miscibility with water and other organic solvents. It is often used for gradient elution in HPLC to improve analytical separations.

Why is 2-Propanol preferred for HPLC applications?
2-Propanol is preferred for HPLC applications because of its low UV cutoff, which makes it suitable for UV detection in HPLC analysis. Additionally, it has low viscosity and volatility, making it ideal for use in HPLC systems without causing system contamination.

How does 2-Propanol affect chromatographic separations in HPLC?
2-Propanol can improve chromatographic separations in HPLC by providing enhanced peak shapes and resolutions. Its ability to reduce peak tailing and increase retention times makes it a popular choice for HPLC method development and optimization.

Is 2-Propanol compatible with different types of detectors in HPLC systems?
2-Propanol is compatible with most detectors used in HPLC systems, including UV-Vis, fluorescence, and mass spectrometry detectors. Its low UV cutoff allows for sensitive detection of compounds in the UV range, making it a versatile solvent for different types of analyses.

Are there any specific precautions to consider when using 2-Propanol in HPLC?
When using 2-Propanol in HPLC, it is important to ensure proper ventilation in the laboratory to prevent exposure to harmful fumes. Additionally, users should be aware of the flammability of 2-Propanol and take necessary precautions to prevent accidents or fires in the lab.
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