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| Name | 2-Propanol for HPLC Gradient |
| Purity | ≥99.9% |
| Grade | HPLC grade |
| Cas Number | 67-63-0 |
| Molecular Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Density | 0.785 g/mL at 25°C |
| Boiling Point | 82.5°C |
| Flash Point | 11°C |
| Appearance | Clear colorless liquid |
| Solubility | Soluble in water, ethanol |
| Storage Condition | Store in a cool, dry, ventilated area away from sources of heat or ignition |
| Shelf Life | 2 years |
| Applications | Used as a solvent in HPLC gradient analysis |
| Packaging | Available in multiple pack sizes including 1L, 4L, and 20L containers |
FAQ
What is 2-Propanol and how is it used in HPLC gradient applications?
2-Propanol, also known as isopropanol or isopropyl alcohol, is a common solvent used in various laboratory applications including liquid chromatography. In HPLC gradient applications, 2-Propanol is often used as a mobile phase solvent to elute compounds from the column based on their interaction with the stationary phase.
Why is 2-Propanol a preferred solvent for HPLC gradient applications?
2-Propanol is a versatile solvent that is miscible with water and many organic solvents, making it ideal for creating gradient elution profiles in HPLC separations. It also has a low UV cut-off, which allows for more sensitive detections of compounds in the UV spectrum. Additionally, 2-Propanol has a low viscosity which helps to reduce back pressure in the HPLC system.
How can 2-Propanol be optimized for use in HPLC gradient experiments?
When using 2-Propanol in HPLC gradient experiments, it is important to ensure that the solvent is of high purity to avoid any contaminants that could interfere with the separation process. It is also crucial to properly degas the solvent to minimize the formation of air bubbles in the system, which could result in poor peak shapes and reproducibility issues. It is recommended to use a suitable buffer system and adjust the pH of the mobile phase to optimize the separation of target compounds.
What are some common challenges associated with using 2-Propanol in HPLC gradient applications?
One common challenge when using 2-Propanol in HPLC gradient applications is the potential for solvent compatibility issues with the stationary phase or instrument components. It is important to regularly check for any signs of precipitation or degradation in the system that could affect the chromatographic performance. Additionally, the use of 2-Propanol in HPLC may require additional precautions due to its flammable nature, such as proper ventilation and storage.
In conclusion, 2-Propanol is a versatile solvent that is well-suited for HPLC gradient applications due to its compatibility with a wide range of compounds and its ability to create efficient separation profiles. By following best practices for optimizing the use of 2-Propanol in HPLC experiments, researchers can achieve reliable and reproducible results in their analyses.
2-Propanol, also known as isopropanol or isopropyl alcohol, is a common solvent used in various laboratory applications including liquid chromatography. In HPLC gradient applications, 2-Propanol is often used as a mobile phase solvent to elute compounds from the column based on their interaction with the stationary phase.
Why is 2-Propanol a preferred solvent for HPLC gradient applications?
2-Propanol is a versatile solvent that is miscible with water and many organic solvents, making it ideal for creating gradient elution profiles in HPLC separations. It also has a low UV cut-off, which allows for more sensitive detections of compounds in the UV spectrum. Additionally, 2-Propanol has a low viscosity which helps to reduce back pressure in the HPLC system.
How can 2-Propanol be optimized for use in HPLC gradient experiments?
When using 2-Propanol in HPLC gradient experiments, it is important to ensure that the solvent is of high purity to avoid any contaminants that could interfere with the separation process. It is also crucial to properly degas the solvent to minimize the formation of air bubbles in the system, which could result in poor peak shapes and reproducibility issues. It is recommended to use a suitable buffer system and adjust the pH of the mobile phase to optimize the separation of target compounds.
What are some common challenges associated with using 2-Propanol in HPLC gradient applications?
One common challenge when using 2-Propanol in HPLC gradient applications is the potential for solvent compatibility issues with the stationary phase or instrument components. It is important to regularly check for any signs of precipitation or degradation in the system that could affect the chromatographic performance. Additionally, the use of 2-Propanol in HPLC may require additional precautions due to its flammable nature, such as proper ventilation and storage.
In conclusion, 2-Propanol is a versatile solvent that is well-suited for HPLC gradient applications due to its compatibility with a wide range of compounds and its ability to create efficient separation profiles. By following best practices for optimizing the use of 2-Propanol in HPLC experiments, researchers can achieve reliable and reproducible results in their analyses.
