Back
| Name | 2-Propanol |
| Common Name | Isopropanol |
| Chemical Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Boiling Point | 82.6°C |
| Melting Point | -89.5°C |
| Density | 0.786 g/cm3 |
| Solubility | Miscible in water |
| Purity | ≥ 99.5% |
| Storage Conditions | Store at room temperature |
| Applications | Used as a solvent, cleaning agent, and disinfectant |
| Synonyms | Isopropyl alcohol, IPA |
| Appearance | Colorless liquid |
| Flammability | Flammable |
| Hazards | Harmful if swallowed, may cause skin irritation |
FAQ
What is 2-Propanol and how is it used in molecular biology research?
2-Propanol, also known as isopropyl alcohol, is a common laboratory reagent that is widely used in molecular biology research. It is often used for DNA and RNA extraction, protein precipitation, and as a disinfectant for laboratory surfaces and equipment.
How does 2-Propanol differ from other alcohols commonly used in molecular biology?
2-Propanol is preferred over ethanol in some applications due to its lower toxicity and volatility. It also has a higher affinity for water, making it more effective for precipitating nucleic acids and proteins. Additionally, 2-Propanol is less likely to denature biomolecules compared to other alcohols.
What are some common applications of 2-Propanol in molecular biology?
2-Propanol is commonly used for precipitating nucleic acids in DNA and RNA extraction protocols, as it helps separate the nucleic acids from other cellular components. It is also used for protein precipitation, which can help concentrate and purify proteins for downstream analyses. Additionally, 2-Propanol is used as a disinfectant to clean laboratory equipment and surfaces.
How should 2-Propanol be stored and handled in the laboratory?
2-Propanol should be stored in a well-ventilated area away from heat, sparks, and open flames, as it is flammable. It should also be kept in a tightly sealed container to prevent evaporation. When handling 2-Propanol, personal protective equipment such as gloves and safety goggles should be worn to prevent skin contact and inhalation of vapors.
Are there any safety considerations when using 2-Propanol in the laboratory?
2-Propanol should be handled with care due to its flammable and toxic properties. It should not be ingested or inhaled, and contact with skin and eyes should be avoided. In case of accidental exposure, immediate medical attention should be sought. Additionally, 2-Propanol should be used in a fume hood to minimize inhalation of vapors.
2-Propanol, also known as isopropyl alcohol, is a common laboratory reagent that is widely used in molecular biology research. It is often used for DNA and RNA extraction, protein precipitation, and as a disinfectant for laboratory surfaces and equipment.
How does 2-Propanol differ from other alcohols commonly used in molecular biology?
2-Propanol is preferred over ethanol in some applications due to its lower toxicity and volatility. It also has a higher affinity for water, making it more effective for precipitating nucleic acids and proteins. Additionally, 2-Propanol is less likely to denature biomolecules compared to other alcohols.
What are some common applications of 2-Propanol in molecular biology?
2-Propanol is commonly used for precipitating nucleic acids in DNA and RNA extraction protocols, as it helps separate the nucleic acids from other cellular components. It is also used for protein precipitation, which can help concentrate and purify proteins for downstream analyses. Additionally, 2-Propanol is used as a disinfectant to clean laboratory equipment and surfaces.
How should 2-Propanol be stored and handled in the laboratory?
2-Propanol should be stored in a well-ventilated area away from heat, sparks, and open flames, as it is flammable. It should also be kept in a tightly sealed container to prevent evaporation. When handling 2-Propanol, personal protective equipment such as gloves and safety goggles should be worn to prevent skin contact and inhalation of vapors.
Are there any safety considerations when using 2-Propanol in the laboratory?
2-Propanol should be handled with care due to its flammable and toxic properties. It should not be ingested or inhaled, and contact with skin and eyes should be avoided. In case of accidental exposure, immediate medical attention should be sought. Additionally, 2-Propanol should be used in a fume hood to minimize inhalation of vapors.
