Ethanol absolute for molecular biology

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Product Name Ethanol absolute
Product Type Chemical reagent
Purity Absolute
Grade Molecular biology grade
Synonyms Ethyl alcohol
Cas Number 64-17-5
Molecular Formula C2H5OH
Molecular Weight 46.07 g/mol
Appearance Colorless liquid
Boiling Point 78.37 °C
Melting Point -114.1 °C
Density 0.789 g/cm3
Flash Point Mouse: 14.0°C
Solubility Miscible in water, ethanol
Storage Condition Store at room temperature
Hazards Highly flammable, harmful if swallowed, may cause skin irritation
FAQ
What is ethanol absolute and how is it used in molecular biology?
Ethanol absolute is a highly concentrated form of ethanol that is commonly used in molecular biology for a variety of applications. It is often used to precipitate nucleic acids such as DNA and RNA, as well as to sterilize equipment and surfaces in the laboratory.

What are some key benefits of using ethanol absolute in molecular biology?
Ethanol absolute is preferred for molecular biology applications due to its high purity and concentration, which ensures reliable and reproducible results. It is also relatively inexpensive and widely available, making it a cost-effective choice for research laboratories.

How is ethanol absolute different from other forms of ethanol?
Ethanol absolute, also known as anhydrous ethanol, contains very low levels of water and is therefore more concentrated than other forms of ethanol. This high concentration makes it ideal for use in molecular biology, where even small amounts of impurities can significantly impact experimental outcomes.

What are some best practices for handling and storing ethanol absolute in the laboratory?
When working with ethanol absolute, it is important to use appropriate personal protective equipment, such as gloves and safety goggles, to prevent skin and eye contact. It should also be stored in a cool, dry place away from sources of ignition, as it is highly flammable. Additionally, ethanol absolute should be used in a well-ventilated area to minimize exposure to its vapors.

Are there any safety considerations to keep in mind when using ethanol absolute in the laboratory?
Yes, ethanol absolute should be handled with care due to its flammable nature. It should never be mixed with oxidizing agents or strong acids, as this can result in dangerous reactions. Additionally, spills should be cleaned up immediately using appropriate absorbent materials and disposal methods.
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