Brain Heart Infusion Agar (BHI) (Dehydrated Culture Media) for microbiology

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Product Name Brain Heart Infusion Agar (BHI) (Dehydrated Culture Media)
Common Uses Used for the cultivation of a wide variety of fastidious microorganisms, including bacteria, yeast, and fungi.
Composition Peptone, beef heart infusion, proteose peptone, dextrose, sodium chloride, and agar.
PH Range 7.4 - 7.6
Appearance Light beige to tan powder
Solubility Soluble in distilled water
Sterility Sterile
Storage Conditions Store in a cool, dry place away from direct light
Shelf Life Up to 3 years if stored properly
Manufacturer Various microbiology media suppliers
Packaging Available in different package sizes ranging from 500g to 5kg
Preparation Requires reconstitution with distilled water and sterilization before use
Quality Control Tested for performance consistency and sterility before distribution
Microbial Sensitivity Supports growth of a wide range of microorganisms, making it a versatile culture media
Special Instructions Inoculate and incubate at the recommended temperature and time for optimal results
FAQ
What is Brain Heart Infusion Agar (BHI) used for in microbiology?
BHI is a dehydrated culture media commonly used for the cultivation and isolation of fastidious microorganisms, particularly in clinical and research settings. The rich nutrients present in BHI agar support the growth of a wide variety of bacteria, yeast, and fungi.

How do you prepare Brain Heart Infusion Agar (BHI) for use in microbiology?
To prepare BHI agar, simply mix the dehydrated powder with distilled water according to the manufacturer's instructions. Heat the mixture to dissolve the components and sterilize it by autoclaving. After sterilization, allow the agar to cool to around 45-50°C before pouring it into plates or tubes for solidification.

What are the advantages of using Brain Heart Infusion Agar (BHI) in microbiology?
One of the main advantages of using BHI agar is its ability to support the growth of a wide range of microorganisms, including fastidious species. The rich nutrient composition of BHI agar promotes robust growth and provides a versatile platform for various microbiological applications.

What are some key features of Brain Heart Infusion Agar (BHI) that make it a popular choice in microbiology labs?
BHI agar is known for its excellent support for the growth of anaerobic and aerobic bacteria, yeast, and fungi. Its versatility, reliability, and consistent performance make it a popular choice for routine cultivation, identification, and enumeration of microorganisms in clinical, industrial, and research laboratories.

How can Brain Heart Infusion Agar (BHI) be stored to maintain its efficacy and shelf life?
To maintain the efficacy and shelf life of BHI agar, it should be stored in a cool, dry place away from direct sunlight and moisture. Properly sealed containers should be used to protect the agar from contamination and degradation. Additionally, following the manufacturer's recommended storage conditions can help ensure the stability and quality of the dehydrated culture media.
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