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FAQ
What is CCA Coliforms Chromogenic Agar (ISO 9308-1) and how is it used in
microbiology?
CCA Coliforms Chromogenic Agar is a selective and differential medium used for the simultaneous detection and enumeration of total coliforms and Escherichia coli (E. coli) in water samples, food, and other environmental samples. The medium is based on ISO 9308-1 standard for the detection of coliform bacteria.
How does CCA Coliforms Chromogenic Agar work to differentiate coliforms and E. coli?
The medium contains specific chromogenic substrates that undergo enzymatic hydrolysis by coliforms and E. coli, leading to the formation of distinct colored colonies. Total coliforms typically produce blue-green colonies, while E. coli produces pink to magenta colonies. This color differentiation allows for easy identification and enumeration of coliforms and E. coli in the sample.
What are the advantages of using CCA Coliforms Chromogenic Agar in microbiology testing?
One of the main advantages of using CCA Coliforms Chromogenic Agar is its ability to quickly and accurately differentiate coliforms and E. coli colonies based on color, thereby simplifying the identification process. Additionally, the selective nature of the medium inhibits the growth of non-coliform bacteria, reducing the risk of false-positive results.
How is CCA Coliforms Chromogenic Agar prepared and incubated for microbiological analysis?
CCA Coliforms Chromogenic Agar is available in pre-poured, sterile petri dishes with a diameter of 55 mm, making it convenient for use in the laboratory. To prepare the medium, simply allow the plates to come to room temperature before inoculating with the sample. Once inoculated, the plates are then incubated at the appropriate temperature for the specified time according to ISO 9308-1 standards.
What are the key applications of CCA Coliforms Chromogenic Agar in microbiology testing?
CCA Coliforms Chromogenic Agar is commonly used in various microbiological testing applications, including the analysis of water quality, food safety, and environmental monitoring. The medium's ability to specifically detect and differentiate coliforms and E. coli makes it a valuable tool for ensuring the safety and quality of various samples in different industries.
CCA Coliforms Chromogenic Agar is a selective and differential medium used for the simultaneous detection and enumeration of total coliforms and Escherichia coli (E. coli) in water samples, food, and other environmental samples. The medium is based on ISO 9308-1 standard for the detection of coliform bacteria.
How does CCA Coliforms Chromogenic Agar work to differentiate coliforms and E. coli?
The medium contains specific chromogenic substrates that undergo enzymatic hydrolysis by coliforms and E. coli, leading to the formation of distinct colored colonies. Total coliforms typically produce blue-green colonies, while E. coli produces pink to magenta colonies. This color differentiation allows for easy identification and enumeration of coliforms and E. coli in the sample.
What are the advantages of using CCA Coliforms Chromogenic Agar in microbiology testing?
One of the main advantages of using CCA Coliforms Chromogenic Agar is its ability to quickly and accurately differentiate coliforms and E. coli colonies based on color, thereby simplifying the identification process. Additionally, the selective nature of the medium inhibits the growth of non-coliform bacteria, reducing the risk of false-positive results.
How is CCA Coliforms Chromogenic Agar prepared and incubated for microbiological analysis?
CCA Coliforms Chromogenic Agar is available in pre-poured, sterile petri dishes with a diameter of 55 mm, making it convenient for use in the laboratory. To prepare the medium, simply allow the plates to come to room temperature before inoculating with the sample. Once inoculated, the plates are then incubated at the appropriate temperature for the specified time according to ISO 9308-1 standards.
What are the key applications of CCA Coliforms Chromogenic Agar in microbiology testing?
CCA Coliforms Chromogenic Agar is commonly used in various microbiological testing applications, including the analysis of water quality, food safety, and environmental monitoring. The medium's ability to specifically detect and differentiate coliforms and E. coli makes it a valuable tool for ensuring the safety and quality of various samples in different industries.
