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| Product Name | DMEM F12 |
| Brand | Thermo Fisher Scientific |
| Volume | 500 ml |
| Storage Condition | Store at 2-8°C |
| Shelf Life | 24 months |
| PH Range | 7.2 - 7.6 |
| Osmolality | 295 - 335 mOsm/kg |
| Buffer System | Hepes and sodium bicarbonate |
| Nutrients | Contains all essential and non-essential amino acids |
| Supplements | Requires addition of serum or other supplements for cell growth |
| Applications | Used for maintenance and growth of various cell types |
FAQ
What is DMEM F12?
DMEM F12 is a popular cell culture medium that is commonly used for the growth of various types of mammalian cells. It is a mixture of two commonly used basal media, Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 Nutrient Mixture.
What are the advantages of using DMEM F12?
DMEM F12 provides a well-balanced combination of nutrients, vitamins, and salts that support the growth and proliferation of a wide range of cells. It is also versatile and can be used for both adherent and suspension cultures. Additionally, DMEM F12 can be supplemented with various additives such as serum, growth factors, and antibiotics to meet the specific requirements of different cell types.
How is DMEM F12 different from other cell culture media?
DMEM F12 offers a unique formulation that combines the strengths of both DMEM and F-12 media. It contains higher levels of amino acids, trace elements, and other essential nutrients compared to other basal media, making it an ideal choice for supporting the growth of many cell types. Additionally, DMEM F12 has a lower concentration of bicarbonate, which may help to maintain a more stable pH in the culture environment.
Can DMEM F12 be used for long-term cell culture?
Yes, DMEM F12 is suitable for long-term cell culture applications. With the addition of appropriate supplements and regular media changes, cells can be maintained in DMEM F12 for extended periods of time. It is important to monitor cell growth, viability, and morphology to ensure that the culture remains healthy and productive over time.
Are there any special considerations when using DMEM F12?
When using DMEM F12, it is important to follow the manufacturer's recommendations for preparation and storage of the medium. It is also essential to maintain proper sterile techniques during handling to prevent contamination of the cell culture. Additionally, users should be aware of the specific requirements of the cells being cultured and adjust the supplementation of DMEM F12 accordingly to ensure optimal growth and viability.
DMEM F12 is a popular cell culture medium that is commonly used for the growth of various types of mammalian cells. It is a mixture of two commonly used basal media, Dulbecco's Modified Eagle Medium (DMEM) and Ham's F-12 Nutrient Mixture.
What are the advantages of using DMEM F12?
DMEM F12 provides a well-balanced combination of nutrients, vitamins, and salts that support the growth and proliferation of a wide range of cells. It is also versatile and can be used for both adherent and suspension cultures. Additionally, DMEM F12 can be supplemented with various additives such as serum, growth factors, and antibiotics to meet the specific requirements of different cell types.
How is DMEM F12 different from other cell culture media?
DMEM F12 offers a unique formulation that combines the strengths of both DMEM and F-12 media. It contains higher levels of amino acids, trace elements, and other essential nutrients compared to other basal media, making it an ideal choice for supporting the growth of many cell types. Additionally, DMEM F12 has a lower concentration of bicarbonate, which may help to maintain a more stable pH in the culture environment.
Can DMEM F12 be used for long-term cell culture?
Yes, DMEM F12 is suitable for long-term cell culture applications. With the addition of appropriate supplements and regular media changes, cells can be maintained in DMEM F12 for extended periods of time. It is important to monitor cell growth, viability, and morphology to ensure that the culture remains healthy and productive over time.
Are there any special considerations when using DMEM F12?
When using DMEM F12, it is important to follow the manufacturer's recommendations for preparation and storage of the medium. It is also essential to maintain proper sterile techniques during handling to prevent contamination of the cell culture. Additionally, users should be aware of the specific requirements of the cells being cultured and adjust the supplementation of DMEM F12 accordingly to ensure optimal growth and viability.
