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| Name | L-Alanyl-L-Glutamine for cell culture |
| Type | Cell culture supplement |
| Form | Powder |
| Concentration | 200mM |
| Storage Condition | Store at -20°C |
| Shelf Life | 2 years |
| Purity | ≥ 98% |
| Source | Synthetic |
| Solubility | Soluble in water |
| Appearance | White crystalline powder |
| Application | Used as a stable and non-toxic form of L-glutamine for cell culture |
| Usage Recommendation | Add to cell culture media at a final concentration of 4mM |
| Sterility | Sterile-filtered |
FAQ
What is L-Alanyl-L-Glutamine and how is it used in cell culture
applications?
L-Alanyl-L-Glutamine, also known as Ala-Gln, is a dipeptide molecule composed of alanine and glutamine. It is commonly used as a stable source of glutamine in cell culture media to support cell growth and viability.
What are the benefits of using L-Alanyl-L-Glutamine in cell culture compared to traditional methods of adding glutamine separately?
L-Alanyl-L-Glutamine offers several advantages over traditional methods of adding glutamine separately to cell culture media. One of the main benefits is the increased stability of L-Alanyl-L-Glutamine, which eliminates the need for frequent replenishment of glutamine in the media. This stability helps maintain optimal cell growth conditions and minimizes the risk of cell stress or death due to glutamine depletion. Additionally, L-Alanyl-L-Glutamine is highly soluble and does not require pH adjustment, making it easier to use and reducing the risk of pH-induced cell damage.
How does L-Alanyl-L-Glutamine support cell growth and viability in cell culture?
L-Alanyl-L-Glutamine is an essential nutrient for many types of cells in culture, as it is a precursor for nucleic acids, amino acids, and proteins. Glutamine plays a crucial role in supporting cell metabolism, energy production, and protein synthesis. By providing a stable source of glutamine, L-Alanyl-L-Glutamine helps maintain the optimal conditions for cell growth and viability. Additionally, the dipeptide form of glutamine offers enhanced stability and bioavailability compared to free glutamine, further promoting healthy cell growth and proliferation.
Can L-Alanyl-L-Glutamine be used in different types of cell culture systems and applications?
Yes, L-Alanyl-L-Glutamine is suitable for a wide range of cell culture systems and applications, including mammalian cell culture, insect cell culture, and bacterial cell culture. It can be used in both adherent and suspension cell cultures, as well as in serum-containing or serum-free media formulations. The versatility of L-Alanyl-L-Glutamine makes it a valuable tool for researchers working with various cell types and culture conditions.
Are there any special considerations to keep in mind when using L-Alanyl-L-Glutamine in cell culture?
While L-Alanyl-L-Glutamine is generally safe and easy to use in cell culture, there are a few important considerations to keep in mind. It is recommended to follow the manufacturer's instructions for preparation and storage of L-Alanyl-L-Glutamine to ensure maximum stability and efficacy. Additionally, researchers should take care to avoid contamination of cell cultures with bacteria or other microorganisms when using L-Alanyl-L-Glutamine, as this could affect cell health and experimental results. Overall, by following best practices and guidelines for cell culture, researchers can maximize the benefits of using L-Alanyl-L-Glutamine in their experiments.
L-Alanyl-L-Glutamine, also known as Ala-Gln, is a dipeptide molecule composed of alanine and glutamine. It is commonly used as a stable source of glutamine in cell culture media to support cell growth and viability.
What are the benefits of using L-Alanyl-L-Glutamine in cell culture compared to traditional methods of adding glutamine separately?
L-Alanyl-L-Glutamine offers several advantages over traditional methods of adding glutamine separately to cell culture media. One of the main benefits is the increased stability of L-Alanyl-L-Glutamine, which eliminates the need for frequent replenishment of glutamine in the media. This stability helps maintain optimal cell growth conditions and minimizes the risk of cell stress or death due to glutamine depletion. Additionally, L-Alanyl-L-Glutamine is highly soluble and does not require pH adjustment, making it easier to use and reducing the risk of pH-induced cell damage.
How does L-Alanyl-L-Glutamine support cell growth and viability in cell culture?
L-Alanyl-L-Glutamine is an essential nutrient for many types of cells in culture, as it is a precursor for nucleic acids, amino acids, and proteins. Glutamine plays a crucial role in supporting cell metabolism, energy production, and protein synthesis. By providing a stable source of glutamine, L-Alanyl-L-Glutamine helps maintain the optimal conditions for cell growth and viability. Additionally, the dipeptide form of glutamine offers enhanced stability and bioavailability compared to free glutamine, further promoting healthy cell growth and proliferation.
Can L-Alanyl-L-Glutamine be used in different types of cell culture systems and applications?
Yes, L-Alanyl-L-Glutamine is suitable for a wide range of cell culture systems and applications, including mammalian cell culture, insect cell culture, and bacterial cell culture. It can be used in both adherent and suspension cell cultures, as well as in serum-containing or serum-free media formulations. The versatility of L-Alanyl-L-Glutamine makes it a valuable tool for researchers working with various cell types and culture conditions.
Are there any special considerations to keep in mind when using L-Alanyl-L-Glutamine in cell culture?
While L-Alanyl-L-Glutamine is generally safe and easy to use in cell culture, there are a few important considerations to keep in mind. It is recommended to follow the manufacturer's instructions for preparation and storage of L-Alanyl-L-Glutamine to ensure maximum stability and efficacy. Additionally, researchers should take care to avoid contamination of cell cultures with bacteria or other microorganisms when using L-Alanyl-L-Glutamine, as this could affect cell health and experimental results. Overall, by following best practices and guidelines for cell culture, researchers can maximize the benefits of using L-Alanyl-L-Glutamine in their experiments.
