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| Name | MacConkey Broth (Ph. Eur.) (Dehydrated Culture Media) |
| Application | Microbiology |
| Form | Powder |
| Packaging | Bottles |
| Shelf Life | 2 years |
| Storage Conditions | Store in a dry place |
| Composition | Peptone, Lactose, Bile salts, Neutral red, Crystal violet |
| PH Range | 6.9 - 7.3 |
| Purpose | Selective differential medium for the detection of coliform bacteria in water, food, and clinical samples |
| Manufacturing Standard | Ph. Eur. (European Pharmacopoeia) |
| Preparation Instructions | Dissolve in distilled water and sterilize by autoclaving |
FAQ
What is MacConkey Broth (Ph. Eur.) and how is it used in microbiology?
MacConkey Broth (Ph. Eur.) is a dehydrated culture media used in microbiology for the selective isolation and enumeration of Enterobacteriaceae. It is based on the original MacConkey agar formulation developed by Alfred Theodore MacConkey in the 20th century. This broth is a selective and differential medium that allows for the growth of Gram-negative bacteria while inhibiting the growth of Gram-positive bacteria.
What are the key components of MacConkey Broth (Ph. Eur.)?
MacConkey Broth (Ph. Eur.) contains peptones, pancreatic digest of gelatin, bile salts, and lactose as key components. Peptones provide a source of amino acids and nitrogen for bacterial growth, while bile salts inhibit the growth of Gram-positive bacteria. Lactose is used as a fermentable carbohydrate to differentiate lactose-fermenting Enterobacteriaceae from non-lactose-fermenting bacteria.
How is MacConkey Broth (Ph. Eur.) prepared and used in the laboratory?
To prepare MacConkey Broth (Ph. Eur.), the dehydrated powder is reconstituted in distilled water according to the manufacturer's instructions. The broth is then sterilized by autoclaving before use. In the laboratory, MacConkey Broth is commonly used for the detection of coliforms in water samples, as well as for the isolation of Enterobacteriaceae from clinical specimens.
What are the advantages of using MacConkey Broth (Ph. Eur.) in microbiology?
MacConkey Broth (Ph. Eur.) offers several advantages in microbiology. It provides a selective medium for the growth of Enterobacteriaceae, allowing for the differentiation of lactose-fermenting and non-lactose-fermenting bacteria. The broth is easy to prepare and use, making it a convenient option for routine microbiological testing. Additionally, MacConkey Broth can be used in conjunction with other selective and differential media for a comprehensive analysis of bacterial samples.
How does MacConkey Broth (Ph. Eur.) compare to other culture media used in microbiology?
MacConkey Broth (Ph. Eur.) is unique in its selective and differential properties, specifically targeting Enterobacteriaceae. While other culture media may also be used for the isolation of Gram-negative bacteria, MacConkey Broth offers a reliable and specific method for the detection of lactose-fermenting organisms. Additionally, the broth format of MacConkey allows for the detection of bacterial growth without the need for solid agar plates, making it a versatile option for different microbiological applications.
MacConkey Broth (Ph. Eur.) is a dehydrated culture media used in microbiology for the selective isolation and enumeration of Enterobacteriaceae. It is based on the original MacConkey agar formulation developed by Alfred Theodore MacConkey in the 20th century. This broth is a selective and differential medium that allows for the growth of Gram-negative bacteria while inhibiting the growth of Gram-positive bacteria.
What are the key components of MacConkey Broth (Ph. Eur.)?
MacConkey Broth (Ph. Eur.) contains peptones, pancreatic digest of gelatin, bile salts, and lactose as key components. Peptones provide a source of amino acids and nitrogen for bacterial growth, while bile salts inhibit the growth of Gram-positive bacteria. Lactose is used as a fermentable carbohydrate to differentiate lactose-fermenting Enterobacteriaceae from non-lactose-fermenting bacteria.
How is MacConkey Broth (Ph. Eur.) prepared and used in the laboratory?
To prepare MacConkey Broth (Ph. Eur.), the dehydrated powder is reconstituted in distilled water according to the manufacturer's instructions. The broth is then sterilized by autoclaving before use. In the laboratory, MacConkey Broth is commonly used for the detection of coliforms in water samples, as well as for the isolation of Enterobacteriaceae from clinical specimens.
What are the advantages of using MacConkey Broth (Ph. Eur.) in microbiology?
MacConkey Broth (Ph. Eur.) offers several advantages in microbiology. It provides a selective medium for the growth of Enterobacteriaceae, allowing for the differentiation of lactose-fermenting and non-lactose-fermenting bacteria. The broth is easy to prepare and use, making it a convenient option for routine microbiological testing. Additionally, MacConkey Broth can be used in conjunction with other selective and differential media for a comprehensive analysis of bacterial samples.
How does MacConkey Broth (Ph. Eur.) compare to other culture media used in microbiology?
MacConkey Broth (Ph. Eur.) is unique in its selective and differential properties, specifically targeting Enterobacteriaceae. While other culture media may also be used for the isolation of Gram-negative bacteria, MacConkey Broth offers a reliable and specific method for the detection of lactose-fermenting organisms. Additionally, the broth format of MacConkey allows for the detection of bacterial growth without the need for solid agar plates, making it a versatile option for different microbiological applications.
