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Name | Medium 199 with Earle's salts |
Description | Specially formulated medium for cell culture applications |
PH | 7.2 - 7.6 |
Osmolarity | 280-300 mOsm/kg |
Components | Salts, amino acids, vitamins, glucose |
Sterility | Sterile filtered |
Storage | Store at 2-8°C |
Shelf Life | Up to 6 months |
Applications | Cell culture, cell maintenance, cell growth |
Usage | For research or laboratory use only |
Availability | Ready-to-use liquid form |
Manufacturer | Thermo Fisher Scientific |
Catalog Number | A1286401 |
Packaging Size | 500 mL |
Color | Clear to slightly yellow |
Endotoxin Level | <0.5 EU/mL |
Mycoplasma Tested | Yes |
COA | Certificate of Analysis available upon request |
MSDS | Material Safety Data Sheet available online |
Quality Control | Stringent quality control tests performed |
Regulatory Compliance | Manufactured in compliance with cGMP regulations |
Animal Origin | Component free of any animal-derived materials |
Traceability | Lot-specific traceability information provided |
FAQ
What is Medium 199 with Earle's salts?
Medium 199 with Earle's salts is a specialized cell culture medium designed for the in vitro cultivation of a wide range of mammalian cells. It contains Earle's salts, amino acids, vitamins, and other essential nutrients to support robust cell growth and proliferation.
How is Medium 199 with Earle's salts different from other cell culture media?
Medium 199 with Earle's salts is formulated specifically to provide optimal conditions for the growth of mammalian cells. It contains a balanced mixture of nutrients that mimic the physiological environment found in vivo, resulting in superior cell viability, morphology, and overall performance compared to other generic cell culture media.
What types of cells can be cultured in Medium 199 with Earle's salts?
Medium 199 with Earle's salts is suitable for a wide variety of mammalian cells, including primary cells, established cell lines, and stem cells. It can be used for both adherent and suspension cultures, making it a versatile choice for researchers working with different cell types.
How should Medium 199 with Earle's salts be used in cell culture experiments?
Medium 199 with Earle's salts should be used according to the manufacturer's instructions and supplemented with additional components such as serum, growth factors, and antibiotics as needed for specific cell types. Cells should be maintained in a controlled incubator environment with appropriate temperature, pH, and CO2 levels to ensure optimal growth conditions.
What are the benefits of using Medium 199 with Earle's salts in cell culture research?
Medium 199 with Earle's salts offers several advantages for cell culture research, including high cell viability, excellent growth rates, and consistent performance across different cell lines. Its balanced nutrient composition supports the maintenance of cells in vitro, ensuring reliable results and reproducibility in experiments.
Medium 199 with Earle's salts is a specialized cell culture medium designed for the in vitro cultivation of a wide range of mammalian cells. It contains Earle's salts, amino acids, vitamins, and other essential nutrients to support robust cell growth and proliferation.
How is Medium 199 with Earle's salts different from other cell culture media?
Medium 199 with Earle's salts is formulated specifically to provide optimal conditions for the growth of mammalian cells. It contains a balanced mixture of nutrients that mimic the physiological environment found in vivo, resulting in superior cell viability, morphology, and overall performance compared to other generic cell culture media.
What types of cells can be cultured in Medium 199 with Earle's salts?
Medium 199 with Earle's salts is suitable for a wide variety of mammalian cells, including primary cells, established cell lines, and stem cells. It can be used for both adherent and suspension cultures, making it a versatile choice for researchers working with different cell types.
How should Medium 199 with Earle's salts be used in cell culture experiments?
Medium 199 with Earle's salts should be used according to the manufacturer's instructions and supplemented with additional components such as serum, growth factors, and antibiotics as needed for specific cell types. Cells should be maintained in a controlled incubator environment with appropriate temperature, pH, and CO2 levels to ensure optimal growth conditions.
What are the benefits of using Medium 199 with Earle's salts in cell culture research?
Medium 199 with Earle's salts offers several advantages for cell culture research, including high cell viability, excellent growth rates, and consistent performance across different cell lines. Its balanced nutrient composition supports the maintenance of cells in vitro, ensuring reliable results and reproducibility in experiments.