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Name | Mueller-Hinton Broth (Dehydrated Culture Media) |
Product Type | Microbiology culture media |
Composition | Beef extract, acid hydrolysate of casein, starch, dipotassium phosphate |
PH Range | 7.3 - 7.5 |
Appearance | Fine powder |
Sterility | Sterile |
Shelf Life | 2 years |
Storage Conditions | Store in a cool and dry place |
Intended Use | For susceptibility testing of microorganisms by broth microdilution method |
Recommended Usage | Prepare as directed by manufacturer's instructions |
Packaging | Available in various sizes (e.g. 500g, 1kg) |
FAQ
What is Mueller-Hinton Broth (Dehydrated Culture Media) and how is it used in
microbiology?
Mueller-Hinton Broth is a dehydrated culture media commonly used in microbiology for the growth of bacteria. It provides all the essential nutrients needed for bacterial growth, making it an ideal medium for culturing a wide range of bacteria for various purposes such as antibiotic susceptibility testing.
Why is Mueller-Hinton Broth preferred for antibiotic susceptibility testing?
Mueller-Hinton Broth is preferred for antibiotic susceptibility testing due to its low levels of calcium and magnesium ions, which can interfere with the action of antibiotics. This makes the broth an ideal medium for accurately determining the susceptibility of bacteria to various antibiotics.
How is Mueller-Hinton Broth prepared for use in microbiological testing?
To prepare Mueller-Hinton Broth, the dehydrated powder is mixed with distilled water according to the manufacturer's instructions. The mixture is then sterilized by autoclaving to ensure that no contaminants are present before inoculating it with the bacteria of interest for testing.
What are the benefits of using Mueller-Hinton Broth in microbiological testing?
One of the key benefits of using Mueller-Hinton Broth is its consistent and reliable performance in supporting bacterial growth. It is also free from inhibitors that could affect the results of susceptibility testing, making it an ideal choice for accurate and reproducible testing.
Overall, Mueller-Hinton Broth is a versatile and reliable dehydrated culture media that is widely used in microbiology for a variety of applications, especially in antibiotic susceptibility testing. Its high-quality composition and consistent performance make it a valuable tool for researchers and clinicians alike.
Mueller-Hinton Broth is a dehydrated culture media commonly used in microbiology for the growth of bacteria. It provides all the essential nutrients needed for bacterial growth, making it an ideal medium for culturing a wide range of bacteria for various purposes such as antibiotic susceptibility testing.
Why is Mueller-Hinton Broth preferred for antibiotic susceptibility testing?
Mueller-Hinton Broth is preferred for antibiotic susceptibility testing due to its low levels of calcium and magnesium ions, which can interfere with the action of antibiotics. This makes the broth an ideal medium for accurately determining the susceptibility of bacteria to various antibiotics.
How is Mueller-Hinton Broth prepared for use in microbiological testing?
To prepare Mueller-Hinton Broth, the dehydrated powder is mixed with distilled water according to the manufacturer's instructions. The mixture is then sterilized by autoclaving to ensure that no contaminants are present before inoculating it with the bacteria of interest for testing.
What are the benefits of using Mueller-Hinton Broth in microbiological testing?
One of the key benefits of using Mueller-Hinton Broth is its consistent and reliable performance in supporting bacterial growth. It is also free from inhibitors that could affect the results of susceptibility testing, making it an ideal choice for accurate and reproducible testing.
Overall, Mueller-Hinton Broth is a versatile and reliable dehydrated culture media that is widely used in microbiology for a variety of applications, especially in antibiotic susceptibility testing. Its high-quality composition and consistent performance make it a valuable tool for researchers and clinicians alike.