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| Name | RPMI 1640 - Powder medium |
| Form | Powder |
| Preparation | Dissolve in distilled water |
| Suitable For | Cell culture |
| PH Range | 7.2 - 7.4 |
| Osmolarity | 300 mOsm/kg |
| Storage Temperature | 2-8°C |
| Shelf Life | 2 years |
| Sterility | Sterile |
| Endotoxin Level | <0.25 EU/ml |
| Composition | Contains amino acids, vitamins, glucose, and salts |
FAQ
What is RPMI 1640 - Powder medium?
RPMI 1640 is a commonly used cell culture medium that was developed by Roswell Park Memorial Institute (RPMI) in the 1960s. It is a versatile medium that supports the growth of a wide variety of cell types, including both primary cells and established cell lines. The powdered form of RPMI 1640 allows for easy storage and transportation, as it can be reconstituted with water when needed.
What are the key components of RPMI 1640 - Powder medium?
RPMI 1640 contains a variety of essential nutrients, vitamins, and salts that are necessary for cell growth and proliferation. Some of the key components include glucose, amino acids, vitamins, inorganic salts, and buffering agents. RPMI 1640 also typically contains phenol red as a pH indicator.
How is RPMI 1640 - Powder medium typically used in cell culture?
RPMI 1640 is generally used as a basal medium, meaning that it provides the basic nutrients required for cell growth. Researchers can customize RPMI 1640 by adding supplements such as fetal bovine serum, antibiotics, and growth factors to support the specific needs of their cells. The powder form of RPMI 1640 allows for flexibility in preparation, as users can adjust the concentration of the medium as needed.
What are some key advantages of using RPMI 1640 - Powder medium in cell culture?
RPMI 1640 is a widely used medium that has been well-characterized for supporting the growth of many different cell types. Its versatility makes it suitable for a wide range of applications, from basic research to drug discovery. The powdered form of RPMI 1640 also offers convenience and ease of use, as it can be stored in a dry format and reconstituted with water when needed.
Are there any special considerations to keep in mind when using RPMI 1640 - Powder medium?
While RPMI 1640 is a versatile medium, it is important to follow the manufacturer's instructions for reconstitution and storage. Users should also be aware of the specific requirements of their cell lines and adjust the medium accordingly. Additionally, proper aseptic techniques should be followed to prevent contamination and ensure the success of cell culture experiments.
RPMI 1640 is a commonly used cell culture medium that was developed by Roswell Park Memorial Institute (RPMI) in the 1960s. It is a versatile medium that supports the growth of a wide variety of cell types, including both primary cells and established cell lines. The powdered form of RPMI 1640 allows for easy storage and transportation, as it can be reconstituted with water when needed.
What are the key components of RPMI 1640 - Powder medium?
RPMI 1640 contains a variety of essential nutrients, vitamins, and salts that are necessary for cell growth and proliferation. Some of the key components include glucose, amino acids, vitamins, inorganic salts, and buffering agents. RPMI 1640 also typically contains phenol red as a pH indicator.
How is RPMI 1640 - Powder medium typically used in cell culture?
RPMI 1640 is generally used as a basal medium, meaning that it provides the basic nutrients required for cell growth. Researchers can customize RPMI 1640 by adding supplements such as fetal bovine serum, antibiotics, and growth factors to support the specific needs of their cells. The powder form of RPMI 1640 allows for flexibility in preparation, as users can adjust the concentration of the medium as needed.
What are some key advantages of using RPMI 1640 - Powder medium in cell culture?
RPMI 1640 is a widely used medium that has been well-characterized for supporting the growth of many different cell types. Its versatility makes it suitable for a wide range of applications, from basic research to drug discovery. The powdered form of RPMI 1640 also offers convenience and ease of use, as it can be stored in a dry format and reconstituted with water when needed.
Are there any special considerations to keep in mind when using RPMI 1640 - Powder medium?
While RPMI 1640 is a versatile medium, it is important to follow the manufacturer's instructions for reconstitution and storage. Users should also be aware of the specific requirements of their cell lines and adjust the medium accordingly. Additionally, proper aseptic techniques should be followed to prevent contamination and ensure the success of cell culture experiments.
