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| Product Name | SPS Agar (Selective Agar according to Angelotti) (Dehydrated Culture Media) |
| Product Type | Microbiology product |
| Attributes | N/A |
FAQ
What is SPS Agar (Selective Agar according to Angelotti) and its importance in
microbiology?
SPS Agar is a dehydrated culture media used in microbiology for the isolation and enumeration of Staphylococcus aureus in various samples. This selective agar was developed by Angelotti in 1962 and has since been a valuable tool in microbiological research for its specificity towards Staphylococcus aureus.
How does SPS Agar work in selecting for Staphylococcus aureus?
SPS Agar contains selective agents such as lithium chloride and potassium tellurite, which inhibit the growth of other bacteria while allowing Staphylococcus aureus to grow. Additionally, SPS Agar also contains mannitol and phenol red indicators, which help in differentiating Staphylococcus aureus based on their ability to ferment mannitol and produce acid, leading to a change in color of the medium.
What are the advantages of using SPS Agar in microbiological research compared to other selective agars?
SPS Agar offers several advantages over other selective agars for the detection of Staphylococcus aureus. One of the key advantages is its high specificity towards Staphylococcus aureus, providing a reliable and accurate method for their isolation and enumeration. Additionally, the inclusion of indicators in the medium allows for easy differentiation of Staphylococcus aureus based on their metabolic characteristics, making it a valuable tool for both qualitative and quantitative analysis.
How should SPS Agar be prepared and used in the laboratory setting?
To prepare SPS Agar, simply add the specified amount of dehydrated media to the appropriate volume of distilled water, mix well, and sterilize by autoclaving. Once prepared, the agar can be poured into petri dishes and allowed to solidify before inoculating with the sample to be tested. Incubation should be done at the appropriate temperature and for the specified time period to allow for the growth and differentiation of Staphylococcus aureus colonies.
What are the key considerations when using SPS Agar in microbiological research?
When using SPS Agar in microbiological research, it is important to follow the manufacturer's instructions for preparation and use of the medium to ensure optimal results. Additionally, proper aseptic techniques should be employed to prevent contamination of the agar and ensure accurate interpretation of results. Regular quality control checks should also be performed to validate the performance of the medium and confirm its reliability for the detection of Staphylococcus aureus.
SPS Agar is a dehydrated culture media used in microbiology for the isolation and enumeration of Staphylococcus aureus in various samples. This selective agar was developed by Angelotti in 1962 and has since been a valuable tool in microbiological research for its specificity towards Staphylococcus aureus.
How does SPS Agar work in selecting for Staphylococcus aureus?
SPS Agar contains selective agents such as lithium chloride and potassium tellurite, which inhibit the growth of other bacteria while allowing Staphylococcus aureus to grow. Additionally, SPS Agar also contains mannitol and phenol red indicators, which help in differentiating Staphylococcus aureus based on their ability to ferment mannitol and produce acid, leading to a change in color of the medium.
What are the advantages of using SPS Agar in microbiological research compared to other selective agars?
SPS Agar offers several advantages over other selective agars for the detection of Staphylococcus aureus. One of the key advantages is its high specificity towards Staphylococcus aureus, providing a reliable and accurate method for their isolation and enumeration. Additionally, the inclusion of indicators in the medium allows for easy differentiation of Staphylococcus aureus based on their metabolic characteristics, making it a valuable tool for both qualitative and quantitative analysis.
How should SPS Agar be prepared and used in the laboratory setting?
To prepare SPS Agar, simply add the specified amount of dehydrated media to the appropriate volume of distilled water, mix well, and sterilize by autoclaving. Once prepared, the agar can be poured into petri dishes and allowed to solidify before inoculating with the sample to be tested. Incubation should be done at the appropriate temperature and for the specified time period to allow for the growth and differentiation of Staphylococcus aureus colonies.
What are the key considerations when using SPS Agar in microbiological research?
When using SPS Agar in microbiological research, it is important to follow the manufacturer's instructions for preparation and use of the medium to ensure optimal results. Additionally, proper aseptic techniques should be employed to prevent contamination of the agar and ensure accurate interpretation of results. Regular quality control checks should also be performed to validate the performance of the medium and confirm its reliability for the detection of Staphylococcus aureus.
