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| Product Name | Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) (Dehydrated Culture Media) |
| Intended Use | Microbiology |
| Attributes | N/A |
FAQ
What is Sabouraud Glucose Agar with Chloramphenicol (Ph. Eur.)?
Sabouraud Glucose Agar with Chloramphenicol is a dehydrated culture media used in microbiology for the cultivation and isolation of fungi, particularly dermatophytes and yeasts. It contains chloramphenicol, an antibiotic that inhibits the growth of bacteria, allowing for the selective growth of fungi on the agar plate.
What are the advantages of using Sabouraud Glucose Agar with Chloramphenicol?
One of the main advantages of using Sabouraud Glucose Agar with Chloramphenicol is its selective nature, which allows for the targeted growth of fungi while inhibiting the growth of bacteria. This is especially useful when working with clinical specimens that may contain a mix of fungal and bacterial flora. Additionally, the inclusion of chloramphenicol in the agar helps prevent contamination from environmental bacteria during the incubation period.
How is Sabouraud Glucose Agar with Chloramphenicol prepared and used in the laboratory?
To prepare Sabouraud Glucose Agar with Chloramphenicol, the dehydrated media is reconstituted with sterile water according to the manufacturer's instructions. The agar is then poured into petri dishes and allowed to solidify before inoculating with the specimen. After inoculation, the plates are incubated at the appropriate temperature for fungal growth, typically around 25-30°C. The presence of fungal colonies can be visually observed and further analyzed using microscopy or biochemical tests.
What are some common applications of Sabouraud Glucose Agar with Chloramphenicol in microbiology?
Sabouraud Glucose Agar with Chloramphenicol is commonly used in clinical laboratories for the isolation and identification of pathogenic fungi from various clinical specimens, such as skin scrapings, nail clippings, and respiratory samples. It is also used in pharmaceutical laboratories for quality control testing of antifungal agents and in research settings for studying the growth and morphology of fungal species.
Are there any additional precautions or storage requirements for Sabouraud Glucose Agar with Chloramphenicol?
It is important to store Sabouraud Glucose Agar with Chloramphenicol in a cool, dry place away from direct sunlight to prevent moisture absorption and degradation of the media. Additionally, care should be taken to follow aseptic techniques during preparation and handling to avoid contamination. Before use, the agar plates should be checked for any signs of contamination or deterioration, and discarded if necessary.
Sabouraud Glucose Agar with Chloramphenicol is a dehydrated culture media used in microbiology for the cultivation and isolation of fungi, particularly dermatophytes and yeasts. It contains chloramphenicol, an antibiotic that inhibits the growth of bacteria, allowing for the selective growth of fungi on the agar plate.
What are the advantages of using Sabouraud Glucose Agar with Chloramphenicol?
One of the main advantages of using Sabouraud Glucose Agar with Chloramphenicol is its selective nature, which allows for the targeted growth of fungi while inhibiting the growth of bacteria. This is especially useful when working with clinical specimens that may contain a mix of fungal and bacterial flora. Additionally, the inclusion of chloramphenicol in the agar helps prevent contamination from environmental bacteria during the incubation period.
How is Sabouraud Glucose Agar with Chloramphenicol prepared and used in the laboratory?
To prepare Sabouraud Glucose Agar with Chloramphenicol, the dehydrated media is reconstituted with sterile water according to the manufacturer's instructions. The agar is then poured into petri dishes and allowed to solidify before inoculating with the specimen. After inoculation, the plates are incubated at the appropriate temperature for fungal growth, typically around 25-30°C. The presence of fungal colonies can be visually observed and further analyzed using microscopy or biochemical tests.
What are some common applications of Sabouraud Glucose Agar with Chloramphenicol in microbiology?
Sabouraud Glucose Agar with Chloramphenicol is commonly used in clinical laboratories for the isolation and identification of pathogenic fungi from various clinical specimens, such as skin scrapings, nail clippings, and respiratory samples. It is also used in pharmaceutical laboratories for quality control testing of antifungal agents and in research settings for studying the growth and morphology of fungal species.
Are there any additional precautions or storage requirements for Sabouraud Glucose Agar with Chloramphenicol?
It is important to store Sabouraud Glucose Agar with Chloramphenicol in a cool, dry place away from direct sunlight to prevent moisture absorption and degradation of the media. Additionally, care should be taken to follow aseptic techniques during preparation and handling to avoid contamination. Before use, the agar plates should be checked for any signs of contamination or deterioration, and discarded if necessary.
