Sabouraud Glucose Agar (Ph. Eur.) (Dehydrated Culture Media) for microbiology

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Name Sabouraud Glucose Agar (Ph. Eur.) (Dehydrated Culture Media)
For microbiology
Form powder
Presentation dehydrated
Preparation requires reconstitution with sterile water
Composition glucose, peptone, agar
PH Range 5.6 - 6.4
Appearance off-white to cream colored powder
Growth Promotion Test passes test
Sterility passes test
Storage store in a dry place at room temperature
Shelf Life minimum 2 years
Packaging bottles or bags
FAQ
What is Sabouraud Glucose Agar (Ph. Eur.)?

Sabouraud Glucose Agar (Ph.Eur.) is a dehydrated culture media used in microbiology for the isolation and cultivation of fungi. This agar contains glucose as a carbon source and agar as a solidifying agent, providing the necessary nutrients for fungal growth.

How is Sabouraud Glucose Agar (Ph. Eur.) prepared?

To prepare Sabouraud Glucose Agar (Ph.Eur.), the dehydrated powder is mixed with distilled water and heated to dissolve the components. The mixture is then autoclaved to sterilize the media before being poured into petri dishes to solidify.

What are the advantages of using Sabouraud Glucose Agar (Ph. Eur.) for fungal cultivation?

Sabouraud Glucose Agar (Ph.Eur.) is specifically designed to promote the growth of fungi while inhibiting the growth of bacteria, making it an ideal medium for the isolation and identification of fungal species. The selective nature of this agar allows for the accurate assessment of fungal colonies in a mixed microbial population.

How is Sabouraud Glucose Agar (Ph. Eur.) used in microbiological laboratories?

Microbiological laboratories use Sabouraud Glucose Agar (Ph.Eur.) to culture clinical samples containing fungi, such as skin scrapings, hair fragments, or nail clippings. By inoculating the samples onto the agar plates and incubating them at the appropriate temperature, microbiologists can observe and identify the fungal colonies that grow.

What are some tips for using Sabouraud Glucose Agar (Ph. Eur.) effectively in fungal cultures?

For optimal results, it is essential to ensure that the agar plates are prepared and stored correctly to maintain their sterility. Additionally, when inoculating samples onto the agar plates, it is crucial to streak the plates properly to obtain isolated fungal colonies for further analysis. Lastly, incubate the plates at the appropriate temperature and monitor the growth of fungal colonies regularly to identify and characterize the fungal species present in the samples.
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