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Name | Salmonella Shigella Agar (Dehydrated Culture Media) |
Application | Used for the selective isolation of Salmonella and Shigella species from clinical and food samples. |
Composition | Peptones, Yeast Extract, Lactose, Sucrose, Sodium Citrate, Bile Salts, Sodium Thiosulfate, Ferric Ammonium Citrate, Brilliant Green, Sodium Deoxycholate, Agar |
PH | Approx. 7.4 |
Appearance | Light pink to pink |
Solubility | Soluble in distilled water |
Sterility | Sterile |
Storage | Store in a cool, dry place away from direct light |
Shelf Life | Up to 5 years if stored properly |
Packaging | Available in various sizes like 500g, 1kg, etc. |
QC | Tested for performance consistency and purity before release |
FAQ
What is Salmonella Shigella Agar (Dehydrated Culture Media) used for in
microbiology?
Salmonella Shigella Agar is a selective and differential culture media used for the isolation and identification of Salmonella spp. and Shigella spp. from clinical and food samples. It is designed to inhibit the growth of unwanted organisms while facilitating the growth of Salmonella and Shigella species.
How does Salmonella Shigella Agar work?
Salmonella Shigella Agar contains selective agents such as bile salts and dyes that inhibit the growth of gram-positive bacteria and most gram-negative bacteria, allowing for the selective growth of Salmonella and Shigella species. In addition, the differential agents in the media allow for the differentiation of the two pathogenic organisms based on their ability to ferment lactose and produce hydrogen sulfide gas.
What are the advantages of using Salmonella Shigella Agar in microbiology?
Salmonella Shigella Agar provides a reliable and convenient method for the isolation and identification of Salmonella and Shigella species, two common pathogens associated with foodborne illnesses. The selective and differential properties of the media make it easy to interpret results and distinguish between different bacterial species.
How is Salmonella Shigella Agar prepared and used in the laboratory?
Salmonella Shigella Agar is typically prepared by adding the dehydrated media to distilled water and heating to dissolve. The media can then be dispensed into plates or tubes and allowed to solidify before inoculating with the sample. After inoculation, the plates are incubated at the appropriate temperature for the growth of Salmonella and Shigella species, typically 37°C for 24-48 hours.
What are some tips for using Salmonella Shigella Agar effectively in microbiology?
When using Salmonella Shigella Agar, it is important to follow the manufacturer's instructions for preparation and use to ensure optimal results. It is also important to carefully label plates to keep track of samples and maintain proper aseptic techniques to prevent contamination. Additionally, interpreting the results of the agar plates requires knowledge of the characteristic growth patterns and biochemical reactions of Salmonella and Shigella species.
Salmonella Shigella Agar is a selective and differential culture media used for the isolation and identification of Salmonella spp. and Shigella spp. from clinical and food samples. It is designed to inhibit the growth of unwanted organisms while facilitating the growth of Salmonella and Shigella species.
How does Salmonella Shigella Agar work?
Salmonella Shigella Agar contains selective agents such as bile salts and dyes that inhibit the growth of gram-positive bacteria and most gram-negative bacteria, allowing for the selective growth of Salmonella and Shigella species. In addition, the differential agents in the media allow for the differentiation of the two pathogenic organisms based on their ability to ferment lactose and produce hydrogen sulfide gas.
What are the advantages of using Salmonella Shigella Agar in microbiology?
Salmonella Shigella Agar provides a reliable and convenient method for the isolation and identification of Salmonella and Shigella species, two common pathogens associated with foodborne illnesses. The selective and differential properties of the media make it easy to interpret results and distinguish between different bacterial species.
How is Salmonella Shigella Agar prepared and used in the laboratory?
Salmonella Shigella Agar is typically prepared by adding the dehydrated media to distilled water and heating to dissolve. The media can then be dispensed into plates or tubes and allowed to solidify before inoculating with the sample. After inoculation, the plates are incubated at the appropriate temperature for the growth of Salmonella and Shigella species, typically 37°C for 24-48 hours.
What are some tips for using Salmonella Shigella Agar effectively in microbiology?
When using Salmonella Shigella Agar, it is important to follow the manufacturer's instructions for preparation and use to ensure optimal results. It is also important to carefully label plates to keep track of samples and maintain proper aseptic techniques to prevent contamination. Additionally, interpreting the results of the agar plates requires knowledge of the characteristic growth patterns and biochemical reactions of Salmonella and Shigella species.