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| Product Name | TBX Agar (ISO 16649-2,3:2001) (Dehydrated Culture Media) |
| Intended Use | Microbiology |
| Preparation | Dehydrated |
| ISO Standard | 16649-2,3:2001 |
| Form | Agar |
| Storage Conditions | Store in a cool, dry place |
| Shelf Life | 2 years |
| Available Packaging Sizes | 100g, 500g, 1kg |
| Composition | Selective and differential medium for the detection and enumeration of thermotolerant coliform bacteria in water samples |
| PH Range | 6.9 - 7.3 |
| Incubation Temperature | 44.5°C +/- 0.2°C |
| Incubation Time | 24 hours |
| Selectivity | Inhibits growth of non-coliform bacteria |
| Indicator | Colony color change to blue with gas production for coliforms |
| Positive Response | Blue colonies with gas production |
| Negative Response | Colorless colonies without gas production |
| Manufacturer | Various manufacturers |
FAQ
What is TBX Agar (ISO 16649-2,3:2001)?
TBX Agar is a dehydrated culture media used in microbiology for the detection and enumeration of total bacterial colonies in food, water, and environmental samples. This medium is specifically designed to inhibit the growth of non-target organisms while promoting the growth of target bacteria, making it a valuable tool for quality control and research purposes.
What are the key features of TBX Agar?
- Selectivity: TBX Agar is selective for organisms of the genus Salmonella and Escherichia coli, inhibiting the growth of other bacteria and fungi that may be present in the sample.
- Versatility: This medium can be used for both qualitative analysis (presence/absence) and quantitative analysis (enumeration) of bacteria, depending on the specific requirements of the test.
- Easy preparation: TBX Agar is provided in a dehydrated form, which can be easily reconstituted with water to create agar plates for inoculation and incubation.
- Stable performance: With proper storage and handling, TBX Agar maintains its performance over an extended period, ensuring reliable results for routine testing.
- Regulatory compliance: TBX Agar complies with ISO 16649-2,3:2001 standards for microbiological testing, making it suitable for use in laboratories following accreditation guidelines.
How is TBX Agar used in microbiology?
TBX Agar is prepared by adding the dehydrated powder to water, followed by autoclaving to sterilize the medium. Once cooled, the agar is poured into Petri dishes and allowed to solidify. Samples are then inoculated onto the agar surface using a sterile technique, and the plates are incubated at the appropriate temperature for bacterial growth. After the incubation period, colonies can be counted and analyzed for the presence of target organisms.
What are the advantages of using TBX Agar for bacterial enumeration?
- Accuracy: TBX Agar provides a reliable method for enumerating bacterial colonies, allowing for precise quantification of target organisms in a given sample.
- Efficiency: The selective nature of TBX Agar reduces the potential for interference from non-target organisms, streamlining the enumeration process and improving the accuracy of results.
- Standardization: By following established protocols for preparing and using TBX Agar, laboratories can ensure consistent and reproducible results across different tests and operators.
- Data interpretation: The distinct colony morphology of target organisms on TBX Agar allows for easier identification and enumeration, facilitating data interpretation and decision-making in microbiological analysis.
- Cost-effectiveness: TBX Agar offers a cost-effective solution for bacterial enumeration, providing reliable results without the need for additional specialized equipment or reagents.
What are the storage and handling requirements for TBX Agar?
TBX Agar should be stored in a cool, dry place away from direct sunlight and moisture. The dehydrated powder should be protected from contamination and kept sealed when not in use. Once prepared, agar plates should be stored inverted to prevent condensation and microbial contamination. Proper labeling and record-keeping are essential for tracking the expiration date and batch information of the agar medium.
Thank you for your interest in TBX Agar (ISO 16649-2,3:2001). If you have any further questions or would like to place an order, please feel free to contact us.
TBX Agar is a dehydrated culture media used in microbiology for the detection and enumeration of total bacterial colonies in food, water, and environmental samples. This medium is specifically designed to inhibit the growth of non-target organisms while promoting the growth of target bacteria, making it a valuable tool for quality control and research purposes.
What are the key features of TBX Agar?
- Selectivity: TBX Agar is selective for organisms of the genus Salmonella and Escherichia coli, inhibiting the growth of other bacteria and fungi that may be present in the sample.
- Versatility: This medium can be used for both qualitative analysis (presence/absence) and quantitative analysis (enumeration) of bacteria, depending on the specific requirements of the test.
- Easy preparation: TBX Agar is provided in a dehydrated form, which can be easily reconstituted with water to create agar plates for inoculation and incubation.
- Stable performance: With proper storage and handling, TBX Agar maintains its performance over an extended period, ensuring reliable results for routine testing.
- Regulatory compliance: TBX Agar complies with ISO 16649-2,3:2001 standards for microbiological testing, making it suitable for use in laboratories following accreditation guidelines.
How is TBX Agar used in microbiology?
TBX Agar is prepared by adding the dehydrated powder to water, followed by autoclaving to sterilize the medium. Once cooled, the agar is poured into Petri dishes and allowed to solidify. Samples are then inoculated onto the agar surface using a sterile technique, and the plates are incubated at the appropriate temperature for bacterial growth. After the incubation period, colonies can be counted and analyzed for the presence of target organisms.
What are the advantages of using TBX Agar for bacterial enumeration?
- Accuracy: TBX Agar provides a reliable method for enumerating bacterial colonies, allowing for precise quantification of target organisms in a given sample.
- Efficiency: The selective nature of TBX Agar reduces the potential for interference from non-target organisms, streamlining the enumeration process and improving the accuracy of results.
- Standardization: By following established protocols for preparing and using TBX Agar, laboratories can ensure consistent and reproducible results across different tests and operators.
- Data interpretation: The distinct colony morphology of target organisms on TBX Agar allows for easier identification and enumeration, facilitating data interpretation and decision-making in microbiological analysis.
- Cost-effectiveness: TBX Agar offers a cost-effective solution for bacterial enumeration, providing reliable results without the need for additional specialized equipment or reagents.
What are the storage and handling requirements for TBX Agar?
TBX Agar should be stored in a cool, dry place away from direct sunlight and moisture. The dehydrated powder should be protected from contamination and kept sealed when not in use. Once prepared, agar plates should be stored inverted to prevent condensation and microbial contamination. Proper labeling and record-keeping are essential for tracking the expiration date and batch information of the agar medium.
Thank you for your interest in TBX Agar (ISO 16649-2,3:2001). If you have any further questions or would like to place an order, please feel free to contact us.
