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| Product Name | Violet Red Bile Glucose Agar (VRBG) (Ph. Eur.) (ISO21528) (Dehydrated Culture Media) |
| Intended Use | For microbiological testing and enumeration of Enterobacteriaceae in food and water samples |
| Composition | Peptones, yeast extract, bile salts, glucose, neutral red, crystal violet, agar |
| PH Range | 7.3 - 7.5 |
| Appearance | Purple-red colored powder |
| Solubility | Soluble in distilled water |
| Storage Conditions | Store in a dry place at room temperature |
| Shelf Life | Minimum 2 years when stored properly |
| Preparation | Prepare by dissolving specified amount in distilled water, sterilize by autoclaving |
| Quality Control | Tested for pH, appearance, performance and contaminants |
| Packaging | Available in various pack sizes |
FAQ
What is Violet Red Bile Glucose Agar (VRBG) used for in microbiology?
VRBG is a selective and differential medium used for the isolation and enumeration of Enterobacteriaceae from clinical and nonclinical samples. It is commonly used in food and water microbiology for the detection of coliform bacteria, specifically Escherichia coli, due to its ability to differentiate lactose-fermenting colonies.
How does VRBG promote the growth of Enterobacteriaceae?
The selective components of VRBG, including bile salts and crystal violet, inhibit the growth of Gram-positive bacteria and most Gram-negative bacteria, allowing for the selective isolation of Enterobacteriaceae. The presence of glucose as a fermentable carbohydrate and lactose as a differential substrate allow for the differentiation of coliform bacteria based on their ability to ferment these sugars.
What are the advantages of using VRBG in microbiology studies?
VRBG is a reliable medium for the isolation and identification of Enterobacteriaceae, particularly E. coli, due to its ability to selectively inhibit the growth of non-coliform bacteria. It also provides a clear differentiation of lactose-fermenting and non-lactose-fermenting colonies, making it a valuable tool in food and water quality testing.
How should VRBG be prepared and incubated for optimal results?
To prepare VRBG, the dehydrated medium should be reconstituted according to the manufacturer's instructions and sterilized by autoclaving. Once cooled, the medium can be poured into sterile petri dishes and allowed to solidify before inoculation. Inoculated plates should be incubated at 35-37°C for 18-24 hours to allow for the growth and differentiation of bacterial colonies.
What are some important quality control measures to ensure the efficacy of VRBG in microbiology applications?
It is essential to perform regular quality control procedures, such as checking the pH of the prepared medium, testing the growth of control strains on VRBG, and monitoring for any signs of contamination. Additionally, it is recommended to follow standard microbiological practices, including proper storage and handling of the medium, to ensure consistent and reliable results.
VRBG is a selective and differential medium used for the isolation and enumeration of Enterobacteriaceae from clinical and nonclinical samples. It is commonly used in food and water microbiology for the detection of coliform bacteria, specifically Escherichia coli, due to its ability to differentiate lactose-fermenting colonies.
How does VRBG promote the growth of Enterobacteriaceae?
The selective components of VRBG, including bile salts and crystal violet, inhibit the growth of Gram-positive bacteria and most Gram-negative bacteria, allowing for the selective isolation of Enterobacteriaceae. The presence of glucose as a fermentable carbohydrate and lactose as a differential substrate allow for the differentiation of coliform bacteria based on their ability to ferment these sugars.
What are the advantages of using VRBG in microbiology studies?
VRBG is a reliable medium for the isolation and identification of Enterobacteriaceae, particularly E. coli, due to its ability to selectively inhibit the growth of non-coliform bacteria. It also provides a clear differentiation of lactose-fermenting and non-lactose-fermenting colonies, making it a valuable tool in food and water quality testing.
How should VRBG be prepared and incubated for optimal results?
To prepare VRBG, the dehydrated medium should be reconstituted according to the manufacturer's instructions and sterilized by autoclaving. Once cooled, the medium can be poured into sterile petri dishes and allowed to solidify before inoculation. Inoculated plates should be incubated at 35-37°C for 18-24 hours to allow for the growth and differentiation of bacterial colonies.
What are some important quality control measures to ensure the efficacy of VRBG in microbiology applications?
It is essential to perform regular quality control procedures, such as checking the pH of the prepared medium, testing the growth of control strains on VRBG, and monitoring for any signs of contamination. Additionally, it is recommended to follow standard microbiological practices, including proper storage and handling of the medium, to ensure consistent and reliable results.
