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| Name | 2-Nitrophenyl-ß-D-Galactopyranoside |
| Product Type | BioChemica |
| Molecular Formula | C12H15NO8 |
| Molecular Weight | 301.25 g/mol |
| Purity | ≥98% |
| Appearance | White to off-white crystalline powder |
| Solubility | Soluble in water |
| Storage Conditions | Store at -20°C in a dry place |
| Application | Substrate for the detection of ß-galactosidase activity |
| Cas Number | 369-07-3 |
| Pubchem Cid | 197326 |
| Mdl Number | MFCD00005630 |
FAQ
What is 2-Nitrophenyl-ß-D-Galactopyranoside
BioChemica?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica, also known as ONPG, is a synthetic substrate used in biochemical research to detect the presence of the enzyme beta-galactosidase. This enzyme cleaves ONPG into two fragments, releasing a yellow product which can be easily measured spectrophotometrically.
How is 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica used in research?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is commonly used in research to assess the expression of the beta-galactosidase enzyme in bacterial and yeast cells. By measuring the amount of yellow product produced from the cleavage of ONPG, researchers can quantify the activity of beta-galactosidase and study gene expression and regulation.
What are the advantages of using 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica?
One of the main advantages of using 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is its specificity towards beta-galactosidase. This substrate is efficiently cleaved by the enzyme, resulting in a clear colorimetric signal that can be easily detected. Additionally, ONPG is stable and soluble, making it a reliable tool for studies requiring the detection of beta-galactosidase activity.
How is 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica different from other substrates?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica stands out from other substrates due to its specificity for beta-galactosidase. Unlike other substrates that may cross-react with other enzymes, ONPG is specifically cleaved by beta-galactosidase, allowing for accurate measurement of enzyme activity. Additionally, the yellow product formed from the cleavage of ONPG provides a visual indicator of enzyme activity, simplifying detection.
In what applications can 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica be used?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is commonly used in molecular biology and microbiology research for the detection and quantification of beta-galactosidase activity. This substrate can be utilized in enzyme assays, gene expression studies, and reporter gene assays to assess the activity of beta-galactosidase in various biological samples. By using ONPG, researchers can gain insights into gene regulation, protein expression, and cellular processes involving beta-galactosidase.
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica, also known as ONPG, is a synthetic substrate used in biochemical research to detect the presence of the enzyme beta-galactosidase. This enzyme cleaves ONPG into two fragments, releasing a yellow product which can be easily measured spectrophotometrically.
How is 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica used in research?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is commonly used in research to assess the expression of the beta-galactosidase enzyme in bacterial and yeast cells. By measuring the amount of yellow product produced from the cleavage of ONPG, researchers can quantify the activity of beta-galactosidase and study gene expression and regulation.
What are the advantages of using 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica?
One of the main advantages of using 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is its specificity towards beta-galactosidase. This substrate is efficiently cleaved by the enzyme, resulting in a clear colorimetric signal that can be easily detected. Additionally, ONPG is stable and soluble, making it a reliable tool for studies requiring the detection of beta-galactosidase activity.
How is 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica different from other substrates?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica stands out from other substrates due to its specificity for beta-galactosidase. Unlike other substrates that may cross-react with other enzymes, ONPG is specifically cleaved by beta-galactosidase, allowing for accurate measurement of enzyme activity. Additionally, the yellow product formed from the cleavage of ONPG provides a visual indicator of enzyme activity, simplifying detection.
In what applications can 2-Nitrophenyl-ß-D-Galactopyranoside BioChemica be used?
2-Nitrophenyl-ß-D-Galactopyranoside BioChemica is commonly used in molecular biology and microbiology research for the detection and quantification of beta-galactosidase activity. This substrate can be utilized in enzyme assays, gene expression studies, and reporter gene assays to assess the activity of beta-galactosidase in various biological samples. By using ONPG, researchers can gain insights into gene regulation, protein expression, and cellular processes involving beta-galactosidase.
