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| Product Name | 3,3',5,5'-Tetramethylbenzidine |
| CAS Number | 54827-17-7 |
| Molecular Formula | C16H20N2 |
| Molar Mass | 240.34 g/mol |
| Appearance | White to light yellow crystalline powder |
| Solubility | Soluble in organic solvents |
| Melting Point | 169-171°C |
| Boiling Point | 332-334°C |
| Storage | Store in a cool, dry place |
| Uses | Commonly used as a substrate for detecting horseradish peroxidase in ELISA and other immunoassays |
| Synonyms | TMB, TMBZ, 3,3',5,5'-TMB |
FAQ
What is 3,3',5,5'-Tetramethylbenzidine and what is it used
for?
3,3',5,5'-Tetramethylbenzidine, also known as TMB, is a chromogenic substrate commonly used in enzyme-linked immunosorbent assays (ELISAs) and other enzyme-mediated detection systems. It is commonly used to detect the presence of horseradish peroxidase (HRP) activity, which is often used as a marker for the presence of specific molecules in biological samples.
How does 3,3',5,5'-Tetramethylbenzidine work in ELISAs?
In ELISAs, 3,3',5,5'-Tetramethylbenzidine is commonly used as a substrate for HRP. When HRP reacts with hydrogen peroxide in the presence of TMB, the substrate is oxidized and produces a blue color. This color change can be quantified using a spectrophotometer, allowing researchers to measure the amount of bound antibody in their samples.
What are the advantages of using 3,3',5,5'-Tetramethylbenzidine in biochemical assays?
One of the main advantages of using 3,3',5,5'-Tetramethylbenzidine is its high sensitivity, which allows for the detection of low levels of HRP activity. Additionally, TMB produces a stable and long-lasting color change, making it ideal for assays that require long incubation times. Furthermore, TMB is relatively easy to use and can be easily incorporated into existing ELISA protocols.
Are there any limitations or considerations when using 3,3',5,5'-Tetramethylbenzidine in assays?
While 3,3',5,5'-Tetramethylbenzidine is a commonly used substrate in biochemical assays, there are some limitations to consider. TMB is sensitive to light and can degrade if exposed to prolonged or intense light, which can affect the accuracy of the results. Additionally, TMB can produce a high background signal in certain conditions, which may require optimization of the assay conditions to reduce background noise.
Can 3,3',5,5'-Tetramethylbenzidine be used in applications other than ELISAs?
Yes, 3,3',5,5'-Tetramethylbenzidine can be used in other applications beyond ELISAs. TMB is commonly used in other enzyme-mediated assays, such as Western blots, immunohistochemistry, and immunocytochemistry. Additionally, TMB can be used in colorimetric assays to detect the presence of hydrogen peroxide or other peroxidases in biological samples.
3,3',5,5'-Tetramethylbenzidine, also known as TMB, is a chromogenic substrate commonly used in enzyme-linked immunosorbent assays (ELISAs) and other enzyme-mediated detection systems. It is commonly used to detect the presence of horseradish peroxidase (HRP) activity, which is often used as a marker for the presence of specific molecules in biological samples.
How does 3,3',5,5'-Tetramethylbenzidine work in ELISAs?
In ELISAs, 3,3',5,5'-Tetramethylbenzidine is commonly used as a substrate for HRP. When HRP reacts with hydrogen peroxide in the presence of TMB, the substrate is oxidized and produces a blue color. This color change can be quantified using a spectrophotometer, allowing researchers to measure the amount of bound antibody in their samples.
What are the advantages of using 3,3',5,5'-Tetramethylbenzidine in biochemical assays?
One of the main advantages of using 3,3',5,5'-Tetramethylbenzidine is its high sensitivity, which allows for the detection of low levels of HRP activity. Additionally, TMB produces a stable and long-lasting color change, making it ideal for assays that require long incubation times. Furthermore, TMB is relatively easy to use and can be easily incorporated into existing ELISA protocols.
Are there any limitations or considerations when using 3,3',5,5'-Tetramethylbenzidine in assays?
While 3,3',5,5'-Tetramethylbenzidine is a commonly used substrate in biochemical assays, there are some limitations to consider. TMB is sensitive to light and can degrade if exposed to prolonged or intense light, which can affect the accuracy of the results. Additionally, TMB can produce a high background signal in certain conditions, which may require optimization of the assay conditions to reduce background noise.
Can 3,3',5,5'-Tetramethylbenzidine be used in applications other than ELISAs?
Yes, 3,3',5,5'-Tetramethylbenzidine can be used in other applications beyond ELISAs. TMB is commonly used in other enzyme-mediated assays, such as Western blots, immunohistochemistry, and immunocytochemistry. Additionally, TMB can be used in colorimetric assays to detect the presence of hydrogen peroxide or other peroxidases in biological samples.
