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| Product Name | Acrylamide 2K - Solution (18%) |
| Application | Denaturing DNA-PAGE |
| Concentration | 18% |
| Form | Liquid |
| Storage Condition | Store at room temperature |
| Appearance | Clear colorless solution |
| Purity | High purity |
| Solubility | Soluble in water |
| Stability | Stable for long term storage |
| Handling Precautions | Avoid contact with skin and eyes |
| Hazards | May cause skin irritation, eye irritation, respiratory irritation |
| Packaging | Available in various sizes |
| Shelf Life | 1 year |
| Manufacturer | Multiple manufacturers |
| Country Of Origin | Varies |
| Catalog Number | Depends on manufacturer |
| Shipping Restrictions | May have restrictions for certain locations |
| Usage | For denaturing DNA samples before electrophoresis |
FAQ
What is Acrylamide 2K - Solution (18%)
Acrylamide 2K - Solution (18%) is a denaturing agent commonly used in DNA-PAGE (Polyacrylamide Gel Electrophoresis) to separate DNA fragments based on their size. It helps denature the double-stranded DNA molecules into single strands, allowing for better resolution and analysis of DNA samples.
How does Acrylamide 2K - Solution (18%) work?
Acrylamide 2K - Solution (18%) works by disrupting the hydrogen bonds that hold the two strands of DNA together. This denaturing process causes the DNA molecules to separate into single strands, making it easier for them to migrate through the gel matrix during electrophoresis.
What are the advantages of using Acrylamide 2K - Solution (18%) for denaturing DNA-PAGE?
One of the main advantages of using Acrylamide 2K - Solution (18%) is its high denaturation efficiency, which allows for better resolution of DNA fragments. It also helps in achieving sharper and more accurate bands on the gel, making it easier to analyze and interpret the results. Additionally, Acrylamide 2K - Solution (18%) is easy to use and provides consistent results, making it a reliable choice for DNA-PAGE experiments.
How should Acrylamide 2K - Solution (18%) be stored and handled?
Acrylamide 2K - Solution (18%) should be stored at room temperature in a cool, dry place away from direct sunlight. It is important to handle this solution with care, as acrylamide is a potential neurotoxin and can be harmful if ingested or inhaled. Always wear appropriate personal protective equipment when working with Acrylamide 2K - Solution (18%) and follow proper safety protocols to minimize the risk of exposure.
What is the recommended protocol for using Acrylamide 2K - Solution (18%) in DNA-PAGE experiments?
To use Acrylamide 2K - Solution (18%) in DNA-PAGE experiments, first prepare the denaturing gel by mixing the solution with other components such as bis-acrylamide, TBE buffer, and ammonium persulfate. After casting the gel and allowing it to polymerize, load the DNA samples treated with denaturing agents such as formamide and heat them before loading onto the gel. Run the electrophoresis at the appropriate voltage and time to separate the DNA fragments based on size. Finally, stain and visualize the gel using appropriate methods such as ethidium bromide staining and UV transillumination.
Acrylamide 2K - Solution (18%) is a denaturing agent commonly used in DNA-PAGE (Polyacrylamide Gel Electrophoresis) to separate DNA fragments based on their size. It helps denature the double-stranded DNA molecules into single strands, allowing for better resolution and analysis of DNA samples.
How does Acrylamide 2K - Solution (18%) work?
Acrylamide 2K - Solution (18%) works by disrupting the hydrogen bonds that hold the two strands of DNA together. This denaturing process causes the DNA molecules to separate into single strands, making it easier for them to migrate through the gel matrix during electrophoresis.
What are the advantages of using Acrylamide 2K - Solution (18%) for denaturing DNA-PAGE?
One of the main advantages of using Acrylamide 2K - Solution (18%) is its high denaturation efficiency, which allows for better resolution of DNA fragments. It also helps in achieving sharper and more accurate bands on the gel, making it easier to analyze and interpret the results. Additionally, Acrylamide 2K - Solution (18%) is easy to use and provides consistent results, making it a reliable choice for DNA-PAGE experiments.
How should Acrylamide 2K - Solution (18%) be stored and handled?
Acrylamide 2K - Solution (18%) should be stored at room temperature in a cool, dry place away from direct sunlight. It is important to handle this solution with care, as acrylamide is a potential neurotoxin and can be harmful if ingested or inhaled. Always wear appropriate personal protective equipment when working with Acrylamide 2K - Solution (18%) and follow proper safety protocols to minimize the risk of exposure.
What is the recommended protocol for using Acrylamide 2K - Solution (18%) in DNA-PAGE experiments?
To use Acrylamide 2K - Solution (18%) in DNA-PAGE experiments, first prepare the denaturing gel by mixing the solution with other components such as bis-acrylamide, TBE buffer, and ammonium persulfate. After casting the gel and allowing it to polymerize, load the DNA samples treated with denaturing agents such as formamide and heat them before loading onto the gel. Run the electrophoresis at the appropriate voltage and time to separate the DNA fragments based on size. Finally, stain and visualize the gel using appropriate methods such as ethidium bromide staining and UV transillumination.
