Back
Name | Acrylamide 4K - Solution (40%) - Mix 37.5 : 1 |
Appearance | Clear colorless to pale yellow solution |
Concentration | 40% |
Ratio | Mix 37.5 : 1 |
Solvent | Water |
Purity | High purity |
Storage Condition | Store at room temperature |
Solubility | Soluble in water |
Application | Commonly used in polyacrylamide gel electrophoresis |
Stability | Stable under recommended storage conditions |
Molecular Weight | Approximately 4000 g/mol |
FAQ
What is Acrylamide 4K - Solution (40%) - Mix 37.5:1?
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is a highly concentrated solution of acrylamide, a chemical compound commonly used in molecular biology research for gel electrophoresis and protein purification. The solution is diluted at a ratio of 37.5 parts water to 1 part solution to achieve the desired working concentration.
How is Acrylamide 4K - Solution (40%) - Mix 37.5:1 used in molecular biology research?
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is typically used in conjunction with bis-acrylamide to create polyacrylamide gels for protein separation by electrophoresis. The acrylamide solution is mixed with water, a crosslinking agent such as TEMED, and a polymerization initiator such as APS to initiate gel formation. Proteins are then loaded onto the gel and separated based on their size and charge during electrophoresis.
What are some advantages of using Acrylamide 4K - Solution (40%) - Mix 37.5:1 in research?
One of the main advantages of using Acrylamide 4K - Solution (40%) - Mix 37.5:1 is its high purity and consistency, which ensures reproducible results in experiments. The solution is also highly concentrated, reducing the volume of solution required for gel preparation and minimizing waste. Additionally, the mix ratio of 37.5:1 simplifies the dilution process and makes it easy to achieve the desired working concentration.
Are there any safety considerations when working with Acrylamide 4K - Solution (40%) - Mix 37.5:1?
Yes, acrylamide is a known neurotoxin and carcinogen, so it is important to handle it with caution. Always wear appropriate personal protective equipment, such as gloves and lab coat, when working with acrylamide solutions. Additionally, work in a well-ventilated area or under a fume hood to minimize exposure to fumes. Proper disposal of acrylamide-containing waste is also necessary to prevent environmental contamination.
Where can I purchase Acrylamide 4K - Solution (40%) - Mix 37.5:1 for my research?
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is available for purchase from various suppliers specializing in molecular biology and biochemistry reagents. Check with your preferred supplier or search online for options. Make sure to follow all safety guidelines and regulations when purchasing and using acrylamide solutions in your research.
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is a highly concentrated solution of acrylamide, a chemical compound commonly used in molecular biology research for gel electrophoresis and protein purification. The solution is diluted at a ratio of 37.5 parts water to 1 part solution to achieve the desired working concentration.
How is Acrylamide 4K - Solution (40%) - Mix 37.5:1 used in molecular biology research?
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is typically used in conjunction with bis-acrylamide to create polyacrylamide gels for protein separation by electrophoresis. The acrylamide solution is mixed with water, a crosslinking agent such as TEMED, and a polymerization initiator such as APS to initiate gel formation. Proteins are then loaded onto the gel and separated based on their size and charge during electrophoresis.
What are some advantages of using Acrylamide 4K - Solution (40%) - Mix 37.5:1 in research?
One of the main advantages of using Acrylamide 4K - Solution (40%) - Mix 37.5:1 is its high purity and consistency, which ensures reproducible results in experiments. The solution is also highly concentrated, reducing the volume of solution required for gel preparation and minimizing waste. Additionally, the mix ratio of 37.5:1 simplifies the dilution process and makes it easy to achieve the desired working concentration.
Are there any safety considerations when working with Acrylamide 4K - Solution (40%) - Mix 37.5:1?
Yes, acrylamide is a known neurotoxin and carcinogen, so it is important to handle it with caution. Always wear appropriate personal protective equipment, such as gloves and lab coat, when working with acrylamide solutions. Additionally, work in a well-ventilated area or under a fume hood to minimize exposure to fumes. Proper disposal of acrylamide-containing waste is also necessary to prevent environmental contamination.
Where can I purchase Acrylamide 4K - Solution (40%) - Mix 37.5:1 for my research?
Acrylamide 4K - Solution (40%) - Mix 37.5:1 is available for purchase from various suppliers specializing in molecular biology and biochemistry reagents. Check with your preferred supplier or search online for options. Make sure to follow all safety guidelines and regulations when purchasing and using acrylamide solutions in your research.