Acrylamide - Solution (40 %) - Mix 29 : 1 for molecular biology

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Product Name Acrylamide - Solution (40%) - Mix 29 : 1
Concentration 40%
Mix Ratio 29 : 1
Application Molecular biology
0 Chemical formula: C3H5N
1 Molecular weight: 71.08 g/mol
2 Appearance: Clear colorless liquid
3 Solubility: Soluble in water
4 Storage conditions: Store at room temperature
5 Stability: Stable under recommended storage conditions
6 Usage: Electrophoresis applications
7 Shelf life: 2 years
8 Hazards: Causes skin and eye irritation, harmful if swallowed
9 Handling precautions: Use in a well-ventilated area, wear protective gloves and eyewear
FAQ
What is Acrylamide Solution (40%) and how is it used in molecular biology?

Acrylamide Solution (40%) is a ready-to-use solution commonly used in molecular biology for various applications such as protein electrophoresis, DNA sequencing, and polyacrylamide gel electrophoresis. It is typically prepared as a 40% solution in water and can be used directly without the need for additional preparation steps.

What is the recommended mixing ratio for Acrylamide Solution (40%) in molecular biology experiments?

The recommended mixing ratio for Acrylamide Solution (40%) in molecular biology experiments is 29:1, meaning 29 parts of acrylamide solution should be mixed with 1 part of a cross-linking agent such as bis-acrylamide. This ratio ensures proper polymerization of the gel and optimal results in various applications.

What are the advantages of using Acrylamide Solution (40%) in molecular biology research?

One of the main advantages of using Acrylamide Solution (40%) is its convenience and ease of use. The ready-to-use solution eliminates the need for tedious preparation steps, saving time and effort in the laboratory. Additionally, the high purity of the acrylamide ensures reproducible results and consistent performance in experiments.

Are there any safety considerations when working with Acrylamide Solution (40%) in the lab?

Yes, it is important to handle Acrylamide Solution (40%) with caution in the laboratory due to its potential hazards. Acrylamide is a neurotoxin and a potential carcinogen, so appropriate personal protective equipment such as gloves, lab coats, and safety goggles should be worn when working with the solution. It is also important to work in a well-ventilated area and follow proper waste disposal protocols to minimize exposure and environmental impact.

Can Acrylamide Solution (40%) be stored for future use, and if so, what are the recommended storage conditions?

Yes, Acrylamide Solution (40%) can be stored for future use under the right conditions. It should be stored in a dark, cool place away from direct sunlight and heat sources to prevent degradation. It is important to tightly seal the container to avoid evaporation and contamination. Make sure to check the expiration date on the label and discard any expired solutions to ensure optimal performance in experiments.
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