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| Product Name | Albumin (BSA) Fraction V (pH 7.0) |
| Manufacturer | Sigma-Aldrich |
| Product Code | A9647 |
| Grade | Biotechnology grade |
| Purity | ≥98% |
| Molecular Weight | 66.5 kDa |
| Solubility | Soluble in water |
| Storage Conditions | Store at -20°C |
| Appearance | White to off-white amorphous powder |
| Applications | Used in cell culture and biochemical research |
| Shelf Life | 2 years |
| Form | Powder |
| Concentration | 10 mg/mL |
| PH | 7.0 |
| Source | Bovine serum |
| Country Of Origin | United States |
| CAS Number | 9048-46-8 |
| UNSPSC | 12352201 |
FAQ
What is Albumin (BSA) Fraction V (pH 7.0) and why is it important?
Albumin (BSA) Fraction V (pH 7.0) is a high-quality protein fraction that is commonly used in research laboratories for various applications. This fraction is purified from bovine serum albumin (BSA) and is specifically adjusted to pH 7.0 to ensure stability and consistency in experiments. Albumin is a versatile protein that plays a crucial role in many biological processes, making it an essential component in research and scientific studies.
What are some common uses of Albumin (BSA) Fraction V (pH 7.0) in research?
Albumin (BSA) Fraction V (pH 7.0) is widely used in research laboratories for a variety of purposes. Some common uses include:
1. Stabilizing enzyme solutions and biological samples
2. Coating cell culture surfaces to promote cell adhesion and growth
3. Blocking nonspecific binding sites in immunoassays
4. Formulating buffers and media for cell culture and biochemical experiments
5. Standardizing protein concentrations in laboratory assays
What are the advantages of using Albumin (BSA) Fraction V (pH 7.0) over other protein fractions?
There are several advantages to using Albumin (BSA) Fraction V (pH 7.0) in research applications. Some of the key benefits include:
1. High purity: Albumin (BSA) Fraction V is purified to high levels, ensuring minimal contamination and reliable results in experiments.
2. Stability: The protein fraction is adjusted to pH 7.0, which enhances its stability and longevity in storage and experimental conditions.
3. Consistency: Albumin (BSA) Fraction V is standardized to provide consistent results in different experiments, reducing variability and improving reproducibility.
4. Versatility: This protein fraction can be used in a wide range of applications, making it a versatile tool for researchers in various fields.
5. Cost-effective: Albumin (BSA) Fraction V offers a cost-effective solution for protein supplementation and stabilization in laboratory settings.
How should Albumin (BSA) Fraction V (pH 7.0) be stored and handled to maintain its quality?
To ensure the quality and stability of Albumin (BSA) Fraction V (pH 7.0), it is important to follow proper storage and handling procedures. The protein fraction should be stored at -20°C in a dark and dry environment to prevent degradation and contamination. When handling Albumin (BSA) Fraction V, it is recommended to use sterile techniques and avoid repeated freeze-thaw cycles to maintain its integrity. Additionally, it is advisable to aliquot the protein fraction into smaller volumes to minimize exposure to air and reduce the risk of contamination during long-term storage.
What is the recommended concentration of Albumin (BSA) Fraction V (pH 7.0) for various applications in the laboratory?
The optimal concentration of Albumin (BSA) Fraction V (pH 7.0) may vary depending on the specific research application. However, as a general guideline, the protein fraction is typically used at concentrations ranging from 1-10 mg/ml in most experiments. Researchers should titrate the concentration of Albumin (BSA) Fraction V based on the desired outcome and the specific requirements of their experimental protocols. It is important to note that higher concentrations of the protein fraction may be needed for certain applications, such as coating cell culture surfaces or stabilizing enzyme solutions, while lower concentrations may be sufficient for other assays and assays.
Albumin (BSA) Fraction V (pH 7.0) is a high-quality protein fraction that is commonly used in research laboratories for various applications. This fraction is purified from bovine serum albumin (BSA) and is specifically adjusted to pH 7.0 to ensure stability and consistency in experiments. Albumin is a versatile protein that plays a crucial role in many biological processes, making it an essential component in research and scientific studies.
What are some common uses of Albumin (BSA) Fraction V (pH 7.0) in research?
Albumin (BSA) Fraction V (pH 7.0) is widely used in research laboratories for a variety of purposes. Some common uses include:
1. Stabilizing enzyme solutions and biological samples
2. Coating cell culture surfaces to promote cell adhesion and growth
3. Blocking nonspecific binding sites in immunoassays
4. Formulating buffers and media for cell culture and biochemical experiments
5. Standardizing protein concentrations in laboratory assays
What are the advantages of using Albumin (BSA) Fraction V (pH 7.0) over other protein fractions?
There are several advantages to using Albumin (BSA) Fraction V (pH 7.0) in research applications. Some of the key benefits include:
1. High purity: Albumin (BSA) Fraction V is purified to high levels, ensuring minimal contamination and reliable results in experiments.
2. Stability: The protein fraction is adjusted to pH 7.0, which enhances its stability and longevity in storage and experimental conditions.
3. Consistency: Albumin (BSA) Fraction V is standardized to provide consistent results in different experiments, reducing variability and improving reproducibility.
4. Versatility: This protein fraction can be used in a wide range of applications, making it a versatile tool for researchers in various fields.
5. Cost-effective: Albumin (BSA) Fraction V offers a cost-effective solution for protein supplementation and stabilization in laboratory settings.
How should Albumin (BSA) Fraction V (pH 7.0) be stored and handled to maintain its quality?
To ensure the quality and stability of Albumin (BSA) Fraction V (pH 7.0), it is important to follow proper storage and handling procedures. The protein fraction should be stored at -20°C in a dark and dry environment to prevent degradation and contamination. When handling Albumin (BSA) Fraction V, it is recommended to use sterile techniques and avoid repeated freeze-thaw cycles to maintain its integrity. Additionally, it is advisable to aliquot the protein fraction into smaller volumes to minimize exposure to air and reduce the risk of contamination during long-term storage.
What is the recommended concentration of Albumin (BSA) Fraction V (pH 7.0) for various applications in the laboratory?
The optimal concentration of Albumin (BSA) Fraction V (pH 7.0) may vary depending on the specific research application. However, as a general guideline, the protein fraction is typically used at concentrations ranging from 1-10 mg/ml in most experiments. Researchers should titrate the concentration of Albumin (BSA) Fraction V based on the desired outcome and the specific requirements of their experimental protocols. It is important to note that higher concentrations of the protein fraction may be needed for certain applications, such as coating cell culture surfaces or stabilizing enzyme solutions, while lower concentrations may be sufficient for other assays and assays.
