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Product Name | Albumin (BSA) Fraction V (pH 7.0) for Western blotting |
Supplier | Sigma-Aldrich |
Catalog Number | A7030 |
Form | Powder |
Concentration | ≥98% protein basis |
Purity | ≥96% (agarose gel electrophoresis) |
Source | Bovine serum |
Storage | Store at -20°C |
Solubility | Soluble in water |
PH Range | 6.5 - 7.5 |
Applications | Western blotting, ELISA, immunoassays |
Appearance | White to off-white powder |
Molecular Weight | 66.5 kDa |
FAQ
What is the Albumin (BSA) Fraction V (pH 7.0) used for in Western blotting?
Albumin (BSA) Fraction V (pH 7.0) is commonly used as a blocking agent in Western blotting procedures to prevent non-specific binding of antibodies to the membrane.
How does Albumin (BSA) Fraction V (pH 7.0) improve the quality of Western blotting results?
Albumin (BSA) Fraction V (pH 7.0) helps to reduce background noise and improve signal-to-noise ratios in Western blotting by blocking nonspecific binding sites on the membrane.
Is Albumin (BSA) Fraction V (pH 7.0) compatible with different types of antibodies and detection reagents?
Yes, Albumin (BSA) Fraction V (pH 7.0) is compatible with a wide range of antibodies and detection reagents commonly used in Western blotting applications.
What is the recommended concentration of Albumin (BSA) Fraction V (pH 7.0) to use in Western blotting experiments?
The recommended concentration of Albumin (BSA) Fraction V (pH 7.0) for blocking in Western blotting experiments is typically between 1-5% in the blocking buffer.
How should Albumin (BSA) Fraction V (pH 7.0) be stored for optimal performance in Western blotting?
Albumin (BSA) Fraction V (pH 7.0) should be stored at -20°C or below to maintain its stability and integrity for use in Western blotting applications.
Albumin (BSA) Fraction V (pH 7.0) is commonly used as a blocking agent in Western blotting procedures to prevent non-specific binding of antibodies to the membrane.
How does Albumin (BSA) Fraction V (pH 7.0) improve the quality of Western blotting results?
Albumin (BSA) Fraction V (pH 7.0) helps to reduce background noise and improve signal-to-noise ratios in Western blotting by blocking nonspecific binding sites on the membrane.
Is Albumin (BSA) Fraction V (pH 7.0) compatible with different types of antibodies and detection reagents?
Yes, Albumin (BSA) Fraction V (pH 7.0) is compatible with a wide range of antibodies and detection reagents commonly used in Western blotting applications.
What is the recommended concentration of Albumin (BSA) Fraction V (pH 7.0) to use in Western blotting experiments?
The recommended concentration of Albumin (BSA) Fraction V (pH 7.0) for blocking in Western blotting experiments is typically between 1-5% in the blocking buffer.
How should Albumin (BSA) Fraction V (pH 7.0) be stored for optimal performance in Western blotting?
Albumin (BSA) Fraction V (pH 7.0) should be stored at -20°C or below to maintain its stability and integrity for use in Western blotting applications.