Albumin (BSA) Fraction V (pH 7.0) for Western blotting

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Product Name Albumin (BSA) Fraction V (pH 7.0) for Western blotting
Supplier Sigma-Aldrich
Catalog Number A7030
Form Powder
Concentration ≥98% protein basis
Purity ≥96% (agarose gel electrophoresis)
Source Bovine serum
Storage Store at -20°C
Solubility Soluble in water
PH Range 6.5 - 7.5
Applications Western blotting, ELISA, immunoassays
Appearance White to off-white powder
Molecular Weight 66.5 kDa
FAQ
What is the Albumin (BSA) Fraction V (pH 7.0) used for in Western blotting?
Albumin (BSA) Fraction V (pH 7.0) is commonly used as a blocking agent in Western blotting procedures to prevent non-specific binding of antibodies to the membrane.

How does Albumin (BSA) Fraction V (pH 7.0) improve the quality of Western blotting results?
Albumin (BSA) Fraction V (pH 7.0) helps to reduce background noise and improve signal-to-noise ratios in Western blotting by blocking nonspecific binding sites on the membrane.

Is Albumin (BSA) Fraction V (pH 7.0) compatible with different types of antibodies and detection reagents?
Yes, Albumin (BSA) Fraction V (pH 7.0) is compatible with a wide range of antibodies and detection reagents commonly used in Western blotting applications.

What is the recommended concentration of Albumin (BSA) Fraction V (pH 7.0) to use in Western blotting experiments?
The recommended concentration of Albumin (BSA) Fraction V (pH 7.0) for blocking in Western blotting experiments is typically between 1-5% in the blocking buffer.

How should Albumin (BSA) Fraction V (pH 7.0) be stored for optimal performance in Western blotting?
Albumin (BSA) Fraction V (pH 7.0) should be stored at -20°C or below to maintain its stability and integrity for use in Western blotting applications.
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