D-Luciferin Sodium Salt

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Name D-Luciferin Sodium Salt
CAS Number 103404-75-7
Formula C11H7O3SNa
Molar Mass 248.23 g/mol
Appearance White to off-white powder
Solubility Soluble in water
Storage Conditions Store at -20°C, protect from light
Melting Point 240-242°C
Stability Stable under recommended storage conditions
Purity ≥ 98% (HPLC)
Application Substrate for luciferase enzyme in bioluminescence assays
Shipping Information Shipped at room temperature
FAQ
What is D-Luciferin Sodium Salt?

D-Luciferin Sodium Salt is a synthetic compound that is commonly used in bioluminescence imaging studies. It is a substrate for the enzyme luciferase, which generates bioluminescence when it reacts with oxygen, ATP, and Mg2+ ions.

How is D-Luciferin Sodium Salt used in research?

D-Luciferin Sodium Salt is commonly used in research studies to monitor gene expression, track cell migration, and visualize biological processes in living organisms. It is widely used in fields such as cancer research, immunology, and neuroscience.

What are the advantages of using D-Luciferin Sodium Salt in bioluminescence imaging studies?

One of the main advantages of using D-Luciferin Sodium Salt is its high sensitivity, which allows for the detection of low levels of gene expression or cell migration. Additionally, its compatibility with various imaging modalities, such as IVIS imaging systems, makes it a versatile tool for researchers.

Are there any limitations or considerations when using D-Luciferin Sodium Salt in research?

While D-Luciferin Sodium Salt is a powerful tool for bioluminescence imaging studies, there are several limitations to consider. For example, the substrate's stability in aqueous solutions can be affected by factors such as pH and temperature. It is also important to optimize experimental conditions to maximize signal intensity and minimize background noise.

How should D-Luciferin Sodium Salt be stored and handled in the lab?

D-Luciferin Sodium Salt should be stored at -20°C in a dry, dark place to prevent degradation. It is important to protect the substrate from light exposure, as ultraviolet light can cause photochemical reactions that reduce its effectiveness. When handling D-Luciferin Sodium Salt, researchers should wear appropriate personal protective equipment and follow standard laboratory safety protocols.

What are some tips for optimizing bioluminescence imaging experiments using D-Luciferin Sodium Salt?

To optimize bioluminescence imaging experiments using D-Luciferin Sodium Salt, researchers should consider factors such as substrate concentration, luciferase expression levels, and imaging parameters. By carefully controlling these variables and using proper controls, researchers can obtain reliable and reproducible results in their studies.
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