Back
| Product Name | IPTG for Molecular Biology, Dioxane Free |
| Purity | ≥ 99.0% |
| Appearance | White to off-white crystalline powder |
| Solubility | Clear, colorless solution at 100 mg/mL in water |
| Melting Point | NLT 113.0°C |
| Storage | Store at -20°C |
| CAS Number | 3690-83-1 |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.30 g/mol |
| Specificity | Dioxane Free |
| Application | Inducer of β-galactosidase activity in bacterial cells |
| Shelf Life | 2 years |
| Hazard Statements | Harmful if swallowed. Causes skin irritation. Causes serious eye irritation. |
| Precautionary Statements | Wear protective gloves/protective clothing/eye protection/face protection. Wash hands thoroughly after handling. Do not eat, drink or smoke when using this product. |
| Shipping Information | UN1325, Class 4.1, Packing group III |
FAQ
What is IPTG and why is it used in molecular biology experiments?
IPTG, or isopropyl β-D-1-thiogalactopyranoside, is a molecular biology reagent commonly used to induce gene expression in bacterial cells. It is a synthetic analog of lactose, which is an inducer of the lac operon in E. coli. IPTG does not get metabolized by the cell, unlike lactose, making it a more stable and reliable inducer for gene expression studies.
How is IPTG different from other inducers used in molecular biology experiments?
IPTG is different from other inducers in that it is non-metabolizable, and therefore provides a more controlled and consistent induction of gene expression. Other inducers like lactose can be metabolized by the cell, leading to variable results in gene expression studies. IPTG is also dioxane-free, making it a safer and more environmentally friendly option for researchers.
How is IPTG used in molecular biology experiments to induce gene expression?
IPTG is typically added to bacterial cultures at a specific concentration to induce the expression of a target gene controlled by an inducible promoter, such as the lac promoter. The addition of IPTG causes the lac repressor protein to dissociate from the operator sequence, allowing RNA polymerase to transcribe the gene of interest. This results in increased expression of the target gene.
What are the advantages of using dioxane-free IPTG for molecular biology research?
Dioxane-free IPTG offers several advantages for molecular biology research. Firstly, it is safer to use as dioxane is a hazardous chemical that poses health risks to researchers. Additionally, dioxane-free IPTG is more environmentally friendly, as it does not contribute to environmental pollution. Using dioxane-free IPTG ensures the safety of researchers and the sustainability of laboratory practices.
How can researchers ensure the success of their molecular biology experiments when using IPTG?
Researchers can ensure the success of their experiments when using IPTG by carefully optimizing the concentration of the inducer for their specific system. It is important to determine the appropriate concentration of IPTG required for optimal gene induction without causing toxicity to the cells. Additionally, researchers should carefully monitor the expression of the target gene and adjust experimental conditions as needed to achieve the desired results.
IPTG, or isopropyl β-D-1-thiogalactopyranoside, is a molecular biology reagent commonly used to induce gene expression in bacterial cells. It is a synthetic analog of lactose, which is an inducer of the lac operon in E. coli. IPTG does not get metabolized by the cell, unlike lactose, making it a more stable and reliable inducer for gene expression studies.
How is IPTG different from other inducers used in molecular biology experiments?
IPTG is different from other inducers in that it is non-metabolizable, and therefore provides a more controlled and consistent induction of gene expression. Other inducers like lactose can be metabolized by the cell, leading to variable results in gene expression studies. IPTG is also dioxane-free, making it a safer and more environmentally friendly option for researchers.
How is IPTG used in molecular biology experiments to induce gene expression?
IPTG is typically added to bacterial cultures at a specific concentration to induce the expression of a target gene controlled by an inducible promoter, such as the lac promoter. The addition of IPTG causes the lac repressor protein to dissociate from the operator sequence, allowing RNA polymerase to transcribe the gene of interest. This results in increased expression of the target gene.
What are the advantages of using dioxane-free IPTG for molecular biology research?
Dioxane-free IPTG offers several advantages for molecular biology research. Firstly, it is safer to use as dioxane is a hazardous chemical that poses health risks to researchers. Additionally, dioxane-free IPTG is more environmentally friendly, as it does not contribute to environmental pollution. Using dioxane-free IPTG ensures the safety of researchers and the sustainability of laboratory practices.
How can researchers ensure the success of their molecular biology experiments when using IPTG?
Researchers can ensure the success of their experiments when using IPTG by carefully optimizing the concentration of the inducer for their specific system. It is important to determine the appropriate concentration of IPTG required for optimal gene induction without causing toxicity to the cells. Additionally, researchers should carefully monitor the expression of the target gene and adjust experimental conditions as needed to achieve the desired results.
