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| Name | PMSF BioChemica |
| Brand | BioChemica |
| Product Type | Protease inhibitor |
| Packaging | Powder form |
| Purity | ≥98% |
| Molecular Weight | 174.19 g/mol |
| Solubility | Freely soluble in alcohol and slightly soluble in water |
| Storage Conditions | Store at -20°C, protect from light |
| Usage | Commonly used to inhibit serine proteases |
| Applications | Biochemical research, protein purification |
| Appearance | White crystalline powder |
| Melting Point | 63-64°C |
| Boiling Point | 112°C at 0.01mm Hg |
| Cas Number | 329-98-6 |
| Formula | C7H7FNO2S |
| Density | 1.207 g/cm³ |
| Flash Point | 120°C |
| Water Solubility | Slightly soluble |
FAQ
What is PMSF BioChemica and how is it used in research?
PMSF BioChemica is a widely used protease inhibitor in biochemistry and molecular biology research. It is commonly employed to inhibit serine proteases, such as trypsin, chymotrypsin, and thrombin, which can degrade proteins in samples.
How does PMSF BioChemica work to inhibit proteases?
PMSF BioChemica is a serine protease inhibitor that irreversibly binds to the active site of serine proteases, thereby blocking their activity. By forming a covalent bond with the serine residue in the active site, PMSF effectively inhibits the protease and prevents it from cleaving proteins in the sample.
What are the advantages of using PMSF BioChemica over other protease inhibitors?
PMSF BioChemica is preferred by many researchers for its high specificity to serine proteases, as well as its stability in a wide range of experimental conditions. Unlike some other protease inhibitors, PMSF is relatively easy to handle and shows minimal interference with downstream assays.
How should PMSF BioChemica be stored and handled to maintain its stability and efficacy?
PMSF BioChemica should be stored in a cool, dry place away from light and moisture to prevent degradation. It is also recommended to prepare fresh PMSF solutions for each experiment, as the compound is susceptible to hydrolysis in aqueous solutions. When handling PMSF, it is important to wear appropriate personal protective equipment, such as gloves and safety goggles, due to its potential irritant properties.
Can PMSF BioChemica be used in combination with other protease inhibitors for enhanced protease inhibition?
Yes, researchers often use a combination of different protease inhibitors, including PMSF BioChemica, to achieve broad-spectrum protease inhibition in their samples. By combining inhibitors that target different classes of proteases, such as serine, cysteine, and metalloproteases, researchers can ensure comprehensive protection of protein samples from degradation.
PMSF BioChemica is a widely used protease inhibitor in biochemistry and molecular biology research. It is commonly employed to inhibit serine proteases, such as trypsin, chymotrypsin, and thrombin, which can degrade proteins in samples.
How does PMSF BioChemica work to inhibit proteases?
PMSF BioChemica is a serine protease inhibitor that irreversibly binds to the active site of serine proteases, thereby blocking their activity. By forming a covalent bond with the serine residue in the active site, PMSF effectively inhibits the protease and prevents it from cleaving proteins in the sample.
What are the advantages of using PMSF BioChemica over other protease inhibitors?
PMSF BioChemica is preferred by many researchers for its high specificity to serine proteases, as well as its stability in a wide range of experimental conditions. Unlike some other protease inhibitors, PMSF is relatively easy to handle and shows minimal interference with downstream assays.
How should PMSF BioChemica be stored and handled to maintain its stability and efficacy?
PMSF BioChemica should be stored in a cool, dry place away from light and moisture to prevent degradation. It is also recommended to prepare fresh PMSF solutions for each experiment, as the compound is susceptible to hydrolysis in aqueous solutions. When handling PMSF, it is important to wear appropriate personal protective equipment, such as gloves and safety goggles, due to its potential irritant properties.
Can PMSF BioChemica be used in combination with other protease inhibitors for enhanced protease inhibition?
Yes, researchers often use a combination of different protease inhibitors, including PMSF BioChemica, to achieve broad-spectrum protease inhibition in their samples. By combining inhibitors that target different classes of proteases, such as serine, cysteine, and metalloproteases, researchers can ensure comprehensive protection of protein samples from degradation.
