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| Name | Streptavidin Ultrapure |
| Catalog Number | 89881 |
| Source | Recombinant |
| Purity | Greater than 98% |
| Form | Lyophilized powder |
| Storage Conditions | Store at -20°C |
| Molecular Weight | Approximately 53 kDa |
| Biotin-binding Capacity | Approximately 10-15 nmol/mg protein |
| Specific Activity | Greater than 12 units/mg protein |
| Applications | Protein purification, immunoassays, protein labeling, protein detection |
| Conjugation | Can be conjugated with various fluorescent dyes and enzymes |
| SDS-PAGE | Single major band on SDS-PAGE gel |
| Endotoxin Level | Less than 0.1 EU/µg protein |
| Shipping Conditions | Shipped at ambient temperature |
| Handling Instructions | Avoid repeated freeze-thaw cycles |
| Compatibility | Compatible with a wide range of assays and applications |
FAQ
What is Streptavidin UltraPure and how is it different from regular
streptavidin?
Streptavidin UltraPure is a highly purified form of streptavidin that has been carefully isolated and processed to remove any contaminants or impurities. This results in a product that is more stable, with higher binding capacity and lower nonspecific binding compared to regular streptavidin.
How can Streptavidin UltraPure benefit my research or experiments?
Streptavidin UltraPure is ideal for applications that require high specificity and sensitivity, such as in protein detection, protein purification, and nucleic acid detection. Its high purity and low nonspecific binding characteristics make it a reliable reagent for experiments where accuracy and precision are crucial.
Can Streptavidin UltraPure be used in various research fields?
Yes, Streptavidin UltraPure is versatile and can be used in a wide range of research fields, including molecular biology, biochemistry, cell biology, and immunology. Its high affinity for biotin makes it a valuable tool for researchers working with biotinylated molecules.
How should Streptavidin UltraPure be stored to maintain its stability?
Streptavidin UltraPure should be stored at -20°C in a frost-free freezer. It is important to avoid repeated freeze-thaw cycles as this may affect the stability and performance of the product. By following proper storage guidelines, you can ensure the long-term stability of Streptavidin UltraPure.
Are there any specific protocols or tips for using Streptavidin UltraPure in my experiments?
When using Streptavidin UltraPure, it is important to perform a thorough optimization of experimental conditions to achieve the best results. This may include testing different concentrations, buffer systems, and incubation times to maximize binding efficiency and minimize nonspecific interactions. Additionally, using high-quality biotinylated reagents is essential for optimal performance of Streptavidin UltraPure in your experiments.
Streptavidin UltraPure is a highly purified form of streptavidin that has been carefully isolated and processed to remove any contaminants or impurities. This results in a product that is more stable, with higher binding capacity and lower nonspecific binding compared to regular streptavidin.
How can Streptavidin UltraPure benefit my research or experiments?
Streptavidin UltraPure is ideal for applications that require high specificity and sensitivity, such as in protein detection, protein purification, and nucleic acid detection. Its high purity and low nonspecific binding characteristics make it a reliable reagent for experiments where accuracy and precision are crucial.
Can Streptavidin UltraPure be used in various research fields?
Yes, Streptavidin UltraPure is versatile and can be used in a wide range of research fields, including molecular biology, biochemistry, cell biology, and immunology. Its high affinity for biotin makes it a valuable tool for researchers working with biotinylated molecules.
How should Streptavidin UltraPure be stored to maintain its stability?
Streptavidin UltraPure should be stored at -20°C in a frost-free freezer. It is important to avoid repeated freeze-thaw cycles as this may affect the stability and performance of the product. By following proper storage guidelines, you can ensure the long-term stability of Streptavidin UltraPure.
Are there any specific protocols or tips for using Streptavidin UltraPure in my experiments?
When using Streptavidin UltraPure, it is important to perform a thorough optimization of experimental conditions to achieve the best results. This may include testing different concentrations, buffer systems, and incubation times to maximize binding efficiency and minimize nonspecific interactions. Additionally, using high-quality biotinylated reagents is essential for optimal performance of Streptavidin UltraPure in your experiments.
