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| Product Name | Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica |
| CAS Number | 298-93-1 |
| Formula | C18H16N5SBr |
| Molecular Weight | 414.28 g/mol |
| Appearance | Yellow crystalline powder |
| Solubility | Soluble in DMSO, ethanol |
| Storage | Store at room temperature away from light |
| Shelf Life | 2 years |
| Usage | Cell viability assay |
| Absorption Maximum | 560 nm |
| Assay (HPLC) | ≥ 98% |
| Purity (TLC) | ≥ 97% |
FAQ
What is Thiazolyl Blue Tetrazolium Bromide (MTT) BioChemica and how is it used in
research?
MTT BioChemica is a yellow tetrazolium salt that is commonly used in cell proliferation and cytotoxicity assays. It is a colorimetric assay that measures the metabolic activity of cells, making it a valuable tool for assessing cell viability and cell growth in a variety of research applications. When MTT is added to cells, it is reduced by mitochondrial dehydrogenases in viable cells to form insoluble purple formazan crystals. The amount of formazan produced is directly proportional to the number of viable cells present in the sample, allowing researchers to quickly and accurately quantify cell viability.
How is MTT BioChemica different from other cell viability assays?
MTT BioChemica offers several advantages over other cell viability assays. Firstly, it is a simple and cost-effective assay that requires minimal equipment and expertise to perform. Additionally, MTT is non-toxic to cells and does not interfere with cellular functions, making it suitable for long-term cell culture experiments. The colorimetric readout of MTT is stable and can be measured using a standard plate reader, allowing for high-throughput screening and precise quantification of cell viability. Overall, MTT BioChemica is a reliable and versatile assay that is widely used in cell biology research.
What are the key applications of MTT BioChemica in research?
MTT BioChemica is commonly used in a variety of research applications, including cell proliferation assays, cytotoxicity studies, and drug screening. In cell proliferation assays, MTT is used to assess the growth rate of cells treated with different compounds or growth factors. Researchers can use MTT to evaluate the effects of drugs, toxins, or other molecules on cell viability and metabolic activity. Additionally, MTT is a valuable tool for studying apoptosis, oxidative stress, and other cellular processes. Overall, MTT BioChemica is a versatile assay that can be tailored to suit a wide range of research questions and experimental designs.
How can I optimize my MTT assay for accurate and reproducible results?
To ensure accurate and reproducible results with the MTT assay, there are several key factors to consider. First, it is essential to carefully optimize the incubation time and concentration of MTT for your specific cell type and experimental conditions. Additionally, controlling for factors such as temperature, pH, and light exposure during the assay can help minimize variability and improve the reliability of your results. It is also important to establish appropriate controls and standards to compare your experimental samples against. Finally, using proper data analysis techniques and statistical methods will help you interpret your results effectively and draw valid conclusions from your MTT assays.
What are some common troubleshooting tips for the MTT assay?
If you encounter issues with your MTT assay, there are several common troubleshooting tips that can help you identify and address the problem. If your formazan crystals are not forming or are forming unevenly, it may be due to inadequate solubilization of the crystals or incomplete reduction of MTT by the cells. In this case, you can try adjusting the incubation time, temperature, or MTT concentration to optimize the assay conditions. If your absorbance readings are inconsistent or inaccurate, it may be due to background interference or inadequate washing steps. To address this, carefully optimize your plate reader settings and ensure thorough washing of the cells before adding MTT. By following these troubleshooting tips and optimizing your assay conditions, you can ensure that your MTT assays yield reliable and reproducible results.
MTT BioChemica is a yellow tetrazolium salt that is commonly used in cell proliferation and cytotoxicity assays. It is a colorimetric assay that measures the metabolic activity of cells, making it a valuable tool for assessing cell viability and cell growth in a variety of research applications. When MTT is added to cells, it is reduced by mitochondrial dehydrogenases in viable cells to form insoluble purple formazan crystals. The amount of formazan produced is directly proportional to the number of viable cells present in the sample, allowing researchers to quickly and accurately quantify cell viability.
How is MTT BioChemica different from other cell viability assays?
MTT BioChemica offers several advantages over other cell viability assays. Firstly, it is a simple and cost-effective assay that requires minimal equipment and expertise to perform. Additionally, MTT is non-toxic to cells and does not interfere with cellular functions, making it suitable for long-term cell culture experiments. The colorimetric readout of MTT is stable and can be measured using a standard plate reader, allowing for high-throughput screening and precise quantification of cell viability. Overall, MTT BioChemica is a reliable and versatile assay that is widely used in cell biology research.
What are the key applications of MTT BioChemica in research?
MTT BioChemica is commonly used in a variety of research applications, including cell proliferation assays, cytotoxicity studies, and drug screening. In cell proliferation assays, MTT is used to assess the growth rate of cells treated with different compounds or growth factors. Researchers can use MTT to evaluate the effects of drugs, toxins, or other molecules on cell viability and metabolic activity. Additionally, MTT is a valuable tool for studying apoptosis, oxidative stress, and other cellular processes. Overall, MTT BioChemica is a versatile assay that can be tailored to suit a wide range of research questions and experimental designs.
How can I optimize my MTT assay for accurate and reproducible results?
To ensure accurate and reproducible results with the MTT assay, there are several key factors to consider. First, it is essential to carefully optimize the incubation time and concentration of MTT for your specific cell type and experimental conditions. Additionally, controlling for factors such as temperature, pH, and light exposure during the assay can help minimize variability and improve the reliability of your results. It is also important to establish appropriate controls and standards to compare your experimental samples against. Finally, using proper data analysis techniques and statistical methods will help you interpret your results effectively and draw valid conclusions from your MTT assays.
What are some common troubleshooting tips for the MTT assay?
If you encounter issues with your MTT assay, there are several common troubleshooting tips that can help you identify and address the problem. If your formazan crystals are not forming or are forming unevenly, it may be due to inadequate solubilization of the crystals or incomplete reduction of MTT by the cells. In this case, you can try adjusting the incubation time, temperature, or MTT concentration to optimize the assay conditions. If your absorbance readings are inconsistent or inaccurate, it may be due to background interference or inadequate washing steps. To address this, carefully optimize your plate reader settings and ensure thorough washing of the cells before adding MTT. By following these troubleshooting tips and optimizing your assay conditions, you can ensure that your MTT assays yield reliable and reproducible results.
