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| Name | Fluorescein Sodium |
| Chemical Formula | C20H10 Na2 O5 |
| Molar Mass | 376.27 g/mol |
| CAS Number | 518-47-8 |
| Appearance | Dark orange to red powder |
| Solubility | Soluble in water |
| Melting Point | 314-316°C |
| Boiling Point | Not available |
| Density | Not available |
| Fluorescence | Greenish-yellow fluorescence under UV light |
| Uses | Used as a fluorescent dye in medical diagnostics and ophthalmic procedures |
| Storage | Store in a cool, dry place away from light and moisture |
| Purity | Minimum 95% |
| Refractive Index | 1.589 - 1.661 at 20°C |
| Stability | Stable under normal conditions |
| Odor | Odorless |
FAQ
What is Fluorescein Sodium (C.I. 45350) used for in analysis?
Fluorescein Sodium (C.I. 45350) is commonly used as a fluorescent dye in various analytical techniques, such as fluorescence microscopy, flow cytometry, and high-performance liquid chromatography (HPLC). It is utilized to label and detect biomolecules, proteins, nucleic acids, and other target compounds due to its high selectivity and sensitivity.
How does Fluorescein Sodium (C.I. 45350) work in analysis?
Fluorescein Sodium (C.I. 45350) absorbs light at a specific wavelength, causing excitation to a higher energy state. Upon returning to the ground state, it emits light at a longer wavelength, generating a fluorescent signal that can be detected and quantified. This fluorescence property is exploited in analytical methods to visualize and measure target substances.
What are the advantages of using Fluorescein Sodium (C.I. 45350) in analysis compared to other dyes?
Fluorescein Sodium (C.I. 45350) offers several advantages in analytical applications, including high fluorescence quantum yield, photostability, and low background signal. Its specific excitation and emission spectra enable selective detection of target molecules with minimal interference from other components in the sample. Additionally, its versatility makes it suitable for a wide range of analytical techniques.
How can Fluorescein Sodium (C.I. 45350) be incorporated into different analytical methods?
Fluorescein Sodium (C.I. 45350) can be conjugated to antibodies, nucleotides, or other molecules to specifically target and label the analytes of interest. It can also be used as a fluorescent probe or tracer to track chemical reactions, visualize cellular structures, or monitor biomolecular interactions. Furthermore, Fluorescein Sodium (C.I. 45350) can be incorporated into chromatographic columns or sensor surfaces for sensitive and selective detection.
What are the key considerations for using Fluorescein Sodium (C.I. 45350) in analysis?
When using Fluorescein Sodium (C.I. 45350) in analysis, it is essential to consider factors such as its concentration, labeling efficiency, and compatibility with the analytical method. Careful optimization of excitation and emission settings, as well as control of experimental conditions, can enhance the sensitivity and accuracy of measurements. Additionally, proper storage and handling of Fluorescein Sodium (C.I. 45350) are crucial to maintain its stability and performance in analytical applications.
Fluorescein Sodium (C.I. 45350) is commonly used as a fluorescent dye in various analytical techniques, such as fluorescence microscopy, flow cytometry, and high-performance liquid chromatography (HPLC). It is utilized to label and detect biomolecules, proteins, nucleic acids, and other target compounds due to its high selectivity and sensitivity.
How does Fluorescein Sodium (C.I. 45350) work in analysis?
Fluorescein Sodium (C.I. 45350) absorbs light at a specific wavelength, causing excitation to a higher energy state. Upon returning to the ground state, it emits light at a longer wavelength, generating a fluorescent signal that can be detected and quantified. This fluorescence property is exploited in analytical methods to visualize and measure target substances.
What are the advantages of using Fluorescein Sodium (C.I. 45350) in analysis compared to other dyes?
Fluorescein Sodium (C.I. 45350) offers several advantages in analytical applications, including high fluorescence quantum yield, photostability, and low background signal. Its specific excitation and emission spectra enable selective detection of target molecules with minimal interference from other components in the sample. Additionally, its versatility makes it suitable for a wide range of analytical techniques.
How can Fluorescein Sodium (C.I. 45350) be incorporated into different analytical methods?
Fluorescein Sodium (C.I. 45350) can be conjugated to antibodies, nucleotides, or other molecules to specifically target and label the analytes of interest. It can also be used as a fluorescent probe or tracer to track chemical reactions, visualize cellular structures, or monitor biomolecular interactions. Furthermore, Fluorescein Sodium (C.I. 45350) can be incorporated into chromatographic columns or sensor surfaces for sensitive and selective detection.
What are the key considerations for using Fluorescein Sodium (C.I. 45350) in analysis?
When using Fluorescein Sodium (C.I. 45350) in analysis, it is essential to consider factors such as its concentration, labeling efficiency, and compatibility with the analytical method. Careful optimization of excitation and emission settings, as well as control of experimental conditions, can enhance the sensitivity and accuracy of measurements. Additionally, proper storage and handling of Fluorescein Sodium (C.I. 45350) are crucial to maintain its stability and performance in analytical applications.
