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Name | Phenol Red Sodium Salt |
Chemical Formula | C19H13NaO5S |
Molecular Weight | 376.36 g/mol |
Appearance | Dark red powder |
Solubility | Soluble in water |
PH Range | 6.8 - 8.2 |
Melting Point | >300°C |
Storage Conditions | Store in a cool, dry place |
Usage | pH indicator in cell culture media |
Application | Used to detect changes in pH in biological samples |
Supplier | Sigma-Aldrich |
Cas Number | 143-74-8 |
Risk Phrases | N/A |
Safety Phrases | N/A |
FAQ
What is Phenol Red Sodium Salt and how is it used?
Phenol Red Sodium Salt is a pH indicator dye that changes color in response to pH levels. It is commonly used in cell culture media to monitor the pH of the growth medium. As cells grow and produce waste products, the pH of the media can change, and Phenol Red Sodium Salt provides a convenient way to visually monitor these changes.
How does Phenol Red Sodium Salt work as a pH indicator?
Phenol Red Sodium Salt is a weak acid with a pKa value of approximately 7.9. At a pH below 6.8, Phenol Red Sodium Salt appears yellow, but as the pH increases, it transitions to red at pH 8.2. This color change makes it easy to monitor subtle shifts in pH levels in cell culture media.
What are the advantages of using Phenol Red Sodium Salt in cell culture?
One of the main advantages of using Phenol Red Sodium Salt in cell culture is its convenience. Since it changes color in response to pH levels, researchers can quickly assess the health of their cells by simply looking at the color of the media. This can help to identify potential issues with cell growth and viability early on, allowing for timely intervention.
Are there any limitations or considerations when using Phenol Red Sodium Salt?
While Phenol Red Sodium Salt is a widely used pH indicator in cell culture, there are some limitations to consider. For example, some cells may be sensitive to the presence of Phenol Red Sodium Salt and may exhibit altered growth patterns or behaviors in its presence. Additionally, the color change of Phenol Red Sodium Salt is not reversible, so once the pH of the media has changed, the color will not revert back to its original state.
How should Phenol Red Sodium Salt be stored and handled?
Phenol Red Sodium Salt should be stored in a cool, dry place away from direct sunlight and moisture. It is important to handle Phenol Red Sodium Salt with care, as it can be toxic if ingested or inhaled. When working with Phenol Red Sodium Salt, it is recommended to wear appropriate personal protective equipment, such as gloves and goggles, to prevent exposure. Additionally, proper disposal methods should be followed to ensure the safe handling of Phenol Red Sodium Salt.
Phenol Red Sodium Salt is a pH indicator dye that changes color in response to pH levels. It is commonly used in cell culture media to monitor the pH of the growth medium. As cells grow and produce waste products, the pH of the media can change, and Phenol Red Sodium Salt provides a convenient way to visually monitor these changes.
How does Phenol Red Sodium Salt work as a pH indicator?
Phenol Red Sodium Salt is a weak acid with a pKa value of approximately 7.9. At a pH below 6.8, Phenol Red Sodium Salt appears yellow, but as the pH increases, it transitions to red at pH 8.2. This color change makes it easy to monitor subtle shifts in pH levels in cell culture media.
What are the advantages of using Phenol Red Sodium Salt in cell culture?
One of the main advantages of using Phenol Red Sodium Salt in cell culture is its convenience. Since it changes color in response to pH levels, researchers can quickly assess the health of their cells by simply looking at the color of the media. This can help to identify potential issues with cell growth and viability early on, allowing for timely intervention.
Are there any limitations or considerations when using Phenol Red Sodium Salt?
While Phenol Red Sodium Salt is a widely used pH indicator in cell culture, there are some limitations to consider. For example, some cells may be sensitive to the presence of Phenol Red Sodium Salt and may exhibit altered growth patterns or behaviors in its presence. Additionally, the color change of Phenol Red Sodium Salt is not reversible, so once the pH of the media has changed, the color will not revert back to its original state.
How should Phenol Red Sodium Salt be stored and handled?
Phenol Red Sodium Salt should be stored in a cool, dry place away from direct sunlight and moisture. It is important to handle Phenol Red Sodium Salt with care, as it can be toxic if ingested or inhaled. When working with Phenol Red Sodium Salt, it is recommended to wear appropriate personal protective equipment, such as gloves and goggles, to prevent exposure. Additionally, proper disposal methods should be followed to ensure the safe handling of Phenol Red Sodium Salt.