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| Name | EDTA - Solution pH 8.0 (0.5 M) |
| Product Type | Molecular biology reagent |
| Concentration | 0.5 M |
| PH | 8 |
| Usage | Chelator for divalent cations |
| Storage Conditions | Store at room temperature |
| Available Volume | Various |
| Composition | Ethylenediaminetetraacetic acid |
| Sterility | Non-sterile |
| Stability | Stable for at least 1 year |
| Application | Used in molecular biology for DNA/RNA purification |
| Solubility | Soluble in water |
| Appearance | Clear colorless liquid |
| Purity | High purity |
| Specificity | Chelates metal ions without affecting enzymes |
| Compatibility | Compatible with most molecular biology techniques |
| Manufacturing Process | Manufactured under strict quality control standards |
| Shipping Restrictions | May be restricted in some countries |
| Handling Precautions | Wear appropriate PPE while handling |
FAQ
What is EDTA - Solution pH 8.0 (0.5 M) and how can it be used in molecular biology?
EDTA - Solution pH 8.0 (0.5 M) is a buffer solution commonly used in molecular biology research. It contains ethylenediaminetetraacetic acid (EDTA), a chelating agent that binds to metal ions and helps to stabilize enzymes and other proteins. The pH of this solution is adjusted to 8.0 to provide optimal conditions for many biological processes.
How is EDTA - Solution pH 8.0 (0.5 M) typically used in molecular biology experiments?
EDTA - Solution pH 8.0 (0.5 M) is often used in molecular biology experiments to chelate divalent cations such as magnesium and calcium. This helps to prevent these metal ions from interfering with enzyme activity or nucleic acid stability. The buffer properties of EDTA - Solution pH 8.0 (0.5 M) also help maintain a stable pH in the reaction mixture, which is crucial for many enzymatic reactions.
Can EDTA - Solution pH 8.0 (0.5 M) be used for DNA extraction and purification?
Yes, EDTA - Solution pH 8.0 (0.5 M) is commonly used in DNA extraction and purification protocols. By chelating metal ions, EDTA helps to protect DNA from degradation by nucleases. It also helps to stabilize the DNA during the extraction process, making it easier to isolate and purify intact DNA for downstream applications.
What are some other applications of EDTA - Solution pH 8.0 (0.5 M) in molecular biology?
In addition to DNA extraction and purification, EDTA - Solution pH 8.0 (0.5 M) has several other applications in molecular biology. It can be used to inhibit metal-dependent enzymes, such as DNases and RNases, that might otherwise degrade nucleic acids. EDTA is also used in electrophoresis buffers to prevent metal ion-mediated interactions that could affect the migration of DNA or proteins in the gel.
Is EDTA - Solution pH 8.0 (0.5 M) compatible with other buffers and reagents commonly used in molecular biology experiments?
Yes, EDTA - Solution pH 8.0 (0.5 M) is compatible with most buffers and reagents used in molecular biology experiments. However, it is important to consider the final concentration of EDTA in the reaction mixture and adjust the pH accordingly to ensure optimal performance. Care should also be taken when working with metal-dependent enzymes, as EDTA may inhibit their activity if present in excess.
Overall, EDTA - Solution pH 8.0 (0.5 M) is a versatile and essential reagent in molecular biology research, offering researchers a reliable way to chelate metal ions, stabilize enzymes, and maintain optimal pH conditions in their experiments.
EDTA - Solution pH 8.0 (0.5 M) is a buffer solution commonly used in molecular biology research. It contains ethylenediaminetetraacetic acid (EDTA), a chelating agent that binds to metal ions and helps to stabilize enzymes and other proteins. The pH of this solution is adjusted to 8.0 to provide optimal conditions for many biological processes.
How is EDTA - Solution pH 8.0 (0.5 M) typically used in molecular biology experiments?
EDTA - Solution pH 8.0 (0.5 M) is often used in molecular biology experiments to chelate divalent cations such as magnesium and calcium. This helps to prevent these metal ions from interfering with enzyme activity or nucleic acid stability. The buffer properties of EDTA - Solution pH 8.0 (0.5 M) also help maintain a stable pH in the reaction mixture, which is crucial for many enzymatic reactions.
Can EDTA - Solution pH 8.0 (0.5 M) be used for DNA extraction and purification?
Yes, EDTA - Solution pH 8.0 (0.5 M) is commonly used in DNA extraction and purification protocols. By chelating metal ions, EDTA helps to protect DNA from degradation by nucleases. It also helps to stabilize the DNA during the extraction process, making it easier to isolate and purify intact DNA for downstream applications.
What are some other applications of EDTA - Solution pH 8.0 (0.5 M) in molecular biology?
In addition to DNA extraction and purification, EDTA - Solution pH 8.0 (0.5 M) has several other applications in molecular biology. It can be used to inhibit metal-dependent enzymes, such as DNases and RNases, that might otherwise degrade nucleic acids. EDTA is also used in electrophoresis buffers to prevent metal ion-mediated interactions that could affect the migration of DNA or proteins in the gel.
Is EDTA - Solution pH 8.0 (0.5 M) compatible with other buffers and reagents commonly used in molecular biology experiments?
Yes, EDTA - Solution pH 8.0 (0.5 M) is compatible with most buffers and reagents used in molecular biology experiments. However, it is important to consider the final concentration of EDTA in the reaction mixture and adjust the pH accordingly to ensure optimal performance. Care should also be taken when working with metal-dependent enzymes, as EDTA may inhibit their activity if present in excess.
Overall, EDTA - Solution pH 8.0 (0.5 M) is a versatile and essential reagent in molecular biology research, offering researchers a reliable way to chelate metal ions, stabilize enzymes, and maintain optimal pH conditions in their experiments.
