Back
| Name | EGTA for molecular biology |
| Brand | Thermo Fisher Scientific |
| Size | 100 g |
| Form | Powder |
| Storage Conditions | Store at room temperature |
| Shipping Conditions | Shipped at ambient temperature |
| Applications | Chelating agent for calcium ions |
| Purity | ≥97% |
| Solubility | Soluble in water |
| Cas Number | 15990-43-9 |
| Molecular Weight | 292.24 g/mol |
FAQ
What is EGTA and how is it used in molecular biology?
EGTA, or ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, is a chelating agent that is commonly used in molecular biology to sequester divalent metal ions, such as calcium and magnesium, from solution. By chelating these metal ions, EGTA helps to maintain a low concentration of free metal ions in a solution, which is important for many biochemical reactions that are sensitive to the presence of metal ions.
What are some common applications of EGTA in molecular biology research?
EGTA is commonly used in molecular biology research for a variety of applications. One of the most common uses of EGTA is in cell culture, where it is often added to the growth medium to chelate calcium ions and inhibit the activity of calcium-dependent enzymes. EGTA is also used in the study of intracellular calcium signaling, as it can be used to buffer changes in intracellular calcium levels and investigate the role of calcium in cellular processes.
How does EGTA compare to other chelating agents used in molecular biology?
EGTA is one of the most commonly used chelating agents in molecular biology, due to its high selectivity for calcium ions and its ability to chelate calcium with a high affinity. Compared to other chelating agents, such as EDTA (ethylenediaminetetraacetic acid), EGTA has a much stronger affinity for calcium ions, which makes it particularly useful for studies that require the sequestration of calcium ions from solution.
Can EGTA be used in conjunction with other chelating agents in molecular biology experiments?
Yes, EGTA can be used in conjunction with other chelating agents in molecular biology experiments to achieve a desired level of metal ion chelation. For example, EGTA is often used in combination with EDTA to chelate both calcium and magnesium ions from solution. By adjusting the concentrations of EGTA and other chelating agents, researchers can fine-tune the chelation of metal ions in their experiments to achieve optimal results.
Are there any special considerations to keep in mind when working with EGTA in molecular biology experiments?
When working with EGTA in molecular biology experiments, it is important to keep in mind that EGTA has a high affinity for calcium ions and can chelate other divalent metal ions as well. Therefore, researchers should carefully consider the concentration of EGTA to use in their experiments, taking into account the specific metal ions that need to be chelated. Additionally, EGTA should be stored in a cool, dry place and protected from light to prevent degradation.
EGTA, or ethyleneglycol-bis(2-aminoethylether)-N,N,N',N'-tetraacetic acid, is a chelating agent that is commonly used in molecular biology to sequester divalent metal ions, such as calcium and magnesium, from solution. By chelating these metal ions, EGTA helps to maintain a low concentration of free metal ions in a solution, which is important for many biochemical reactions that are sensitive to the presence of metal ions.
What are some common applications of EGTA in molecular biology research?
EGTA is commonly used in molecular biology research for a variety of applications. One of the most common uses of EGTA is in cell culture, where it is often added to the growth medium to chelate calcium ions and inhibit the activity of calcium-dependent enzymes. EGTA is also used in the study of intracellular calcium signaling, as it can be used to buffer changes in intracellular calcium levels and investigate the role of calcium in cellular processes.
How does EGTA compare to other chelating agents used in molecular biology?
EGTA is one of the most commonly used chelating agents in molecular biology, due to its high selectivity for calcium ions and its ability to chelate calcium with a high affinity. Compared to other chelating agents, such as EDTA (ethylenediaminetetraacetic acid), EGTA has a much stronger affinity for calcium ions, which makes it particularly useful for studies that require the sequestration of calcium ions from solution.
Can EGTA be used in conjunction with other chelating agents in molecular biology experiments?
Yes, EGTA can be used in conjunction with other chelating agents in molecular biology experiments to achieve a desired level of metal ion chelation. For example, EGTA is often used in combination with EDTA to chelate both calcium and magnesium ions from solution. By adjusting the concentrations of EGTA and other chelating agents, researchers can fine-tune the chelation of metal ions in their experiments to achieve optimal results.
Are there any special considerations to keep in mind when working with EGTA in molecular biology experiments?
When working with EGTA in molecular biology experiments, it is important to keep in mind that EGTA has a high affinity for calcium ions and can chelate other divalent metal ions as well. Therefore, researchers should carefully consider the concentration of EGTA to use in their experiments, taking into account the specific metal ions that need to be chelated. Additionally, EGTA should be stored in a cool, dry place and protected from light to prevent degradation.
