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| Product Name | L-Glutamine for cell culture |
| Supplier | Various suppliers |
| Form | Powder |
| Purity | ≥ 98% |
| Storage Conditions | Store at 2-8°C |
| Shelf Life | 2 years |
| Molecular Weight | 146.14 g/mol |
| Solubility | Soluble in water |
| Usage | Cell culture supplement |
| Applications | Used as a cell culture media supplement to support cell growth and viability |
| Appearance | White crystalline powder |
| CAS Number | 56-85-9 |
| EC Number | 200-292-1 |
| HS Code | 29241900 |
| UN Number | Not applicable |
| Handling Precautions | Avoid inhalation, ingestion, and skin contact |
| Manufacturing Process | Synthetically produced |
| Alternative Names | 2,5-Dioxopiperazine-5-acetic acid, L-glutamic acid γ-amide |
FAQ
What is L-Glutamine and why is it important for cell culture?
L-Glutamine is a key amino acid that plays a crucial role in cell culture. It is essential for cell growth, proliferation, and metabolism, making it a vital component of cell culture media. Additionally, L-Glutamine serves as a major energy source for rapidly dividing cells, such as cancer cells. Its presence in cell culture media helps to support cell health and viability.
How can L-Glutamine benefit my cell culture experiments?
By supplementing your cell culture media with L-Glutamine, you can promote optimal cell growth and viability. This is especially important when working with fast-growing cell lines that require a constant source of nutrients to thrive. L-Glutamine also helps to maintain the pH balance of the media, which is crucial for the overall health of the cells. In addition, L-Glutamine can enhance the production of recombinant proteins in cell culture, making it a valuable tool for biotechnology and pharmaceutical research.
What are the potential challenges of using L-Glutamine in cell culture?
One potential challenge of using L-Glutamine in cell culture is its susceptibility to degradation in aqueous solutions. This can lead to the formation of ammonia, which is toxic to cells and can negatively impact cell growth. To address this issue, it is important to store L-Glutamine properly and always use fresh solutions to ensure its effectiveness. Additionally, some cell lines may have specific requirements for L-Glutamine concentrations, so it is important to optimize the media composition for each cell type.
How should I incorporate L-Glutamine into my cell culture experiments?
To incorporate L-Glutamine into your cell culture experiments, you can simply add the amino acid to the media at the recommended concentration. Most cell culture media formulations already contain L-Glutamine, but additional supplementation may be necessary depending on the specific requirements of your cell lines. It is important to follow the manufacturer's guidelines for preparing and storing L-Glutamine solutions to ensure maximum effectiveness. Regularly monitoring cell growth and viability can help you determine if adjustments to the L-Glutamine concentration are needed.
Are there any specific tips for using L-Glutamine in cell culture?
When using L-Glutamine in cell culture, there are a few key tips to keep in mind. Firstly, always prepare fresh L-Glutamine solutions to ensure its stability and effectiveness. Avoid prolonged exposure to light and heat, as this can degrade the amino acid. Additionally, be mindful of the specific requirements of your cell lines and adjust the L-Glutamine concentration accordingly. Regularly monitor cell growth and viability to ensure that the media composition is supporting optimal cell health. By following these tips, you can successfully incorporate L-Glutamine into your cell culture experiments for improved results.
L-Glutamine is a key amino acid that plays a crucial role in cell culture. It is essential for cell growth, proliferation, and metabolism, making it a vital component of cell culture media. Additionally, L-Glutamine serves as a major energy source for rapidly dividing cells, such as cancer cells. Its presence in cell culture media helps to support cell health and viability.
How can L-Glutamine benefit my cell culture experiments?
By supplementing your cell culture media with L-Glutamine, you can promote optimal cell growth and viability. This is especially important when working with fast-growing cell lines that require a constant source of nutrients to thrive. L-Glutamine also helps to maintain the pH balance of the media, which is crucial for the overall health of the cells. In addition, L-Glutamine can enhance the production of recombinant proteins in cell culture, making it a valuable tool for biotechnology and pharmaceutical research.
What are the potential challenges of using L-Glutamine in cell culture?
One potential challenge of using L-Glutamine in cell culture is its susceptibility to degradation in aqueous solutions. This can lead to the formation of ammonia, which is toxic to cells and can negatively impact cell growth. To address this issue, it is important to store L-Glutamine properly and always use fresh solutions to ensure its effectiveness. Additionally, some cell lines may have specific requirements for L-Glutamine concentrations, so it is important to optimize the media composition for each cell type.
How should I incorporate L-Glutamine into my cell culture experiments?
To incorporate L-Glutamine into your cell culture experiments, you can simply add the amino acid to the media at the recommended concentration. Most cell culture media formulations already contain L-Glutamine, but additional supplementation may be necessary depending on the specific requirements of your cell lines. It is important to follow the manufacturer's guidelines for preparing and storing L-Glutamine solutions to ensure maximum effectiveness. Regularly monitoring cell growth and viability can help you determine if adjustments to the L-Glutamine concentration are needed.
Are there any specific tips for using L-Glutamine in cell culture?
When using L-Glutamine in cell culture, there are a few key tips to keep in mind. Firstly, always prepare fresh L-Glutamine solutions to ensure its stability and effectiveness. Avoid prolonged exposure to light and heat, as this can degrade the amino acid. Additionally, be mindful of the specific requirements of your cell lines and adjust the L-Glutamine concentration accordingly. Regularly monitor cell growth and viability to ensure that the media composition is supporting optimal cell health. By following these tips, you can successfully incorporate L-Glutamine into your cell culture experiments for improved results.
