2-Propanol for HPLC gradient

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Product Name 2-Propanol for HPLC Gradient
Purity ≥99.9%
CAS Number 67-63-0
Molecular Formula C3H8O
Molecular Weight 60.10 g/mol
Appearance Colorless liquid
Boiling Point 82.5°C
Melting Point -88.5°C
Density 0.785 g/cm3
Solubility In Water Miscible
HPLC Grade Yes
Packaging Available in various pack sizes
Storage Conditions Store at room temperature away from heat sources and direct sunlight
Shelf Life 2 years
Manufacturer Multiple manufacturers
Applications Suitable for use in HPLC gradients, chromatography, and other analytical applications
FAQ
What is 2-Propanol and how is it used in HPLC gradient?

2-Propanol, also known as isopropanol or isopropyl alcohol, is a common organic solvent used in various laboratory applications, including in the field of analytical chemistry. In the context of HPLC (High Performance Liquid Chromatography), 2-Propanol is often used as a mobile phase component in gradient elution.

Can you explain the benefits of using 2-Propanol in HPLC gradient?

2-Propanol offers several advantages when used in HPLC gradient separations. It has a low UV cut-off point, which makes it suitable for UV detection methods. Additionally, 2-Propanol has a low viscosity and high solvating power, making it an ideal solvent for use in HPLC separations. Furthermore, its compatibility with a wide range of compounds and stationary phases makes it versatile for different analytical applications.

How should 2-Propanol be handled and stored in the laboratory?

When working with 2-Propanol in the laboratory, it is important to take proper safety precautions due to its flammable properties. It should be stored in a cool, well-ventilated area away from heat sources and open flames. Personal protective equipment, such as gloves and goggles, should be worn when handling the solvent. Additionally, it is important to follow proper disposal guidelines for used 2-Propanol to ensure safety and environmental compliance.

Are there any potential challenges or considerations when using 2-Propanol in HPLC gradient separations?

While 2-Propanol is a commonly used solvent in HPLC gradient elution, there are some potential challenges to consider. One such challenge is the need for proper solvent mixing and degassing to prevent bubble formation in the system, which can affect separation performance. Additionally, 2-Propanol may interact with certain compounds or stationary phases, leading to peak broadening or other chromatographic issues. It is important to optimize the solvent composition and conditions for each specific separation to achieve optimal results.

In conclusion, 2-Propanol is a versatile and effective solvent for use in HPLC gradient separations, offering benefits such as low UV cut-off, low viscosity, and high solvating power. By following proper safety guidelines and considering potential challenges, researchers can successfully utilize 2-Propanol in their analytical workflows for efficient and accurate separations.
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