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| Name | 2-Propanol |
| Product Type | Molecular Biology reagent |
| Chemical Formula | C3H8O |
| Molecular Weight | 60.10 g/mol |
| Purity | ≥99% |
| Appearance | Clear colorless liquid |
| Density | 0.785 g/mL at 25°C |
| Boiling Point | 82.5 °C |
| Flash Point | 11 °C |
| Solubility | Miscible with water, ethanol, ether, acetone |
| Storage Conditions | Store at room temperature |
| Applications | DNA/RNA extraction, protein precipitation, cell culture applications |
| Hazards | Highly flammable, causes skin and eye irritation, harmful if swallowed or inhaled |
FAQ
What is 2-Propanol and how is it used in molecular biology?
2-Propanol, also known as isopropanol or isopropyl alcohol, is a commonly used solvent in molecular biology. It is often used for DNA and RNA isolation, as well as in protein precipitation and purification procedures. It is also used for cleansing and sterilizing laboratory equipment and workspaces.
Why is 2-Propanol preferred over other solvents in certain molecular biology applications?
2-Propanol is preferred over other solvents in certain molecular biology applications due to its ability to easily precipitate nucleic acids and other biomolecules. Its moderate volatility and low toxicity make it a safe and effective solvent for use in the lab. Additionally, it is readily available and affordable, making it a popular choice among researchers.
How should 2-Propanol be stored and handled to ensure its effectiveness?
2-Propanol should be stored in a cool, dry place away from direct sunlight and sources of heat. It should be kept in a tightly sealed container to prevent evaporation and contamination. When handling 2-Propanol, it is important to wear appropriate protective gear, such as gloves and safety glasses, to avoid skin contact or inhalation of fumes. Spills should be cleaned up immediately using absorbent materials and proper disposal methods should be followed.
Can 2-Propanol be used in combination with other reagents or solvents in molecular biology experiments?
Yes, 2-Propanol can be used in combination with other reagents or solvents in molecular biology experiments. It is often used in conjunction with other solvents, such as phenol and chloroform, for DNA and RNA extraction procedures. It can also be used in protein purification protocols in combination with reagents like acetone or trichloroacetic acid. Care should be taken to ensure compatibility and proper handling when using 2-Propanol in combination with other chemicals.
What are the potential hazards associated with the use of 2-Propanol in molecular biology research?
Although 2-Propanol is considered a relatively safe solvent, there are still potential hazards associated with its use in molecular biology research. Inhalation of vapors or ingestion of the substance can lead to adverse health effects, including dizziness, headache, and nausea. Skin contact with 2-Propanol can cause irritation and dryness. In case of accidental exposure, it is important to seek medical attention and follow proper first aid procedures. Additionally, proper waste disposal guidelines should be followed to ensure environmental safety.
2-Propanol, also known as isopropanol or isopropyl alcohol, is a commonly used solvent in molecular biology. It is often used for DNA and RNA isolation, as well as in protein precipitation and purification procedures. It is also used for cleansing and sterilizing laboratory equipment and workspaces.
Why is 2-Propanol preferred over other solvents in certain molecular biology applications?
2-Propanol is preferred over other solvents in certain molecular biology applications due to its ability to easily precipitate nucleic acids and other biomolecules. Its moderate volatility and low toxicity make it a safe and effective solvent for use in the lab. Additionally, it is readily available and affordable, making it a popular choice among researchers.
How should 2-Propanol be stored and handled to ensure its effectiveness?
2-Propanol should be stored in a cool, dry place away from direct sunlight and sources of heat. It should be kept in a tightly sealed container to prevent evaporation and contamination. When handling 2-Propanol, it is important to wear appropriate protective gear, such as gloves and safety glasses, to avoid skin contact or inhalation of fumes. Spills should be cleaned up immediately using absorbent materials and proper disposal methods should be followed.
Can 2-Propanol be used in combination with other reagents or solvents in molecular biology experiments?
Yes, 2-Propanol can be used in combination with other reagents or solvents in molecular biology experiments. It is often used in conjunction with other solvents, such as phenol and chloroform, for DNA and RNA extraction procedures. It can also be used in protein purification protocols in combination with reagents like acetone or trichloroacetic acid. Care should be taken to ensure compatibility and proper handling when using 2-Propanol in combination with other chemicals.
What are the potential hazards associated with the use of 2-Propanol in molecular biology research?
Although 2-Propanol is considered a relatively safe solvent, there are still potential hazards associated with its use in molecular biology research. Inhalation of vapors or ingestion of the substance can lead to adverse health effects, including dizziness, headache, and nausea. Skin contact with 2-Propanol can cause irritation and dryness. In case of accidental exposure, it is important to seek medical attention and follow proper first aid procedures. Additionally, proper waste disposal guidelines should be followed to ensure environmental safety.
