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| Product Name | Bis-Tris for buffer solutions |
| Buffer Type | Good's buffer |
| PKa Value | 5.8 |
| Molecular Weight | 209.25 g/mol |
| Chemical Formula | C8H19NO5S |
| Solubility | Soluble in water |
| Appearance | White crystalline powder |
| Purity | ≥ 99% |
| Storage Condition | Store at room temperature |
| PH Range | 5.8-7.0 |
| Usage | Commonly used in protein electrophoresis and chromatography |
| Alternative Names | 2,2-Bis(hydroxymethyl)-2,2',2''-nitrilotriethanol |
| CAS Number | 6976-37-0 |
| Manufacturer | Various manufacturers |
| Availability | Commercially available |
| Applications | Used in research laboratories for buffering protein and nucleic acid solutions |
| Packaging Options | Available in different quantities such as 100g, 500g, and 1kg |
| Handling Precautions | Wear suitable protective clothing and gloves when handling |
| Shelf Life | Long shelf life if stored properly |
FAQ
What is Bis-Tris and how is it used in buffer solutions?
Bis-Tris is a zwitterionic buffer reagent that is commonly used in biochemical and molecular biology research. It acts as a buffering agent to maintain the pH of a solution within a specific range, typically between pH 5.8 and 7.8. Bis-Tris is ideal for use in electrophoresis, enzyme assays, cell culture media, and protein purification due to its chemical stability and pH buffering capacity.
How does Bis-Tris differ from other buffering agents?
Bis-Tris has several advantages over other buffering agents such as Tris and HEPES. One key difference is its superior buffering capacity in the pH range of 5.8 to 7.8, which is often required in biological and biochemical experiments. Additionally, Bis-Tris is less likely to interfere with enzyme activity and has lower UV absorbance compared to Tris buffer, making it ideal for spectroscopic applications.
Can Bis-Tris be used in combination with other buffering agents?
Yes, Bis-Tris can be used in combination with other buffering agents to create custom buffer solutions with specific pH ranges and buffering capacities. For example, Bis-Tris is often combined with MES or HEPES buffers to create buffer systems with extended pH ranges for different applications. This flexibility makes Bis-Tris a versatile tool for buffer preparation in various experimental setups.
What are the storage requirements for Bis-Tris?
Bis-Tris should be stored in a cool, dry place away from light to prevent degradation. The reagent should be kept tightly sealed in its original container to maintain its stability and buffering capacity. It is recommended to store Bis-Tris at room temperature or below to prolong its shelf life and ensure reliable results in buffer preparation.
Can Bis-Tris be used in both research and industrial applications?
Yes, Bis-Tris is commonly used in both research and industrial settings for a wide range of applications. In research laboratories, Bis-Tris is often used in protein purification, enzyme assays, electrophoresis, and cell culture media preparation. In industrial applications, Bis-Tris is used in biopharmaceutical manufacturing, food and beverage production, and quality control processes due to its stable pH buffering capacity and compatibility with various analytical techniques.
Bis-Tris is a zwitterionic buffer reagent that is commonly used in biochemical and molecular biology research. It acts as a buffering agent to maintain the pH of a solution within a specific range, typically between pH 5.8 and 7.8. Bis-Tris is ideal for use in electrophoresis, enzyme assays, cell culture media, and protein purification due to its chemical stability and pH buffering capacity.
How does Bis-Tris differ from other buffering agents?
Bis-Tris has several advantages over other buffering agents such as Tris and HEPES. One key difference is its superior buffering capacity in the pH range of 5.8 to 7.8, which is often required in biological and biochemical experiments. Additionally, Bis-Tris is less likely to interfere with enzyme activity and has lower UV absorbance compared to Tris buffer, making it ideal for spectroscopic applications.
Can Bis-Tris be used in combination with other buffering agents?
Yes, Bis-Tris can be used in combination with other buffering agents to create custom buffer solutions with specific pH ranges and buffering capacities. For example, Bis-Tris is often combined with MES or HEPES buffers to create buffer systems with extended pH ranges for different applications. This flexibility makes Bis-Tris a versatile tool for buffer preparation in various experimental setups.
What are the storage requirements for Bis-Tris?
Bis-Tris should be stored in a cool, dry place away from light to prevent degradation. The reagent should be kept tightly sealed in its original container to maintain its stability and buffering capacity. It is recommended to store Bis-Tris at room temperature or below to prolong its shelf life and ensure reliable results in buffer preparation.
Can Bis-Tris be used in both research and industrial applications?
Yes, Bis-Tris is commonly used in both research and industrial settings for a wide range of applications. In research laboratories, Bis-Tris is often used in protein purification, enzyme assays, electrophoresis, and cell culture media preparation. In industrial applications, Bis-Tris is used in biopharmaceutical manufacturing, food and beverage production, and quality control processes due to its stable pH buffering capacity and compatibility with various analytical techniques.
