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| Product Name | CTAB - Lysis buffer BioChemica |
| Brand | BioChemica |
| Buffer Type | Lysis buffer |
| Composition | CTAB (Cetyltrimethylammonium bromide) |
| Quantity | 500 mL |
| Storage Conditions | Store at room temperature |
| Usage | Used for lysing cells and extracting nucleic acids |
| Appearance | Clear, colorless liquid |
| Packaging | Bottle |
| Recommended Working Concentration | Varies depending on application |
| Safety Information | Avoid contact with skin and eyes. Use in a well-ventilated area |
| Shelf Life | 2 years |
FAQ
What is CTAB?
CTAB, or cetyltrimethylammonium bromide, is a quaternary ammonium salt commonly used in molecular biology and biochemistry. It is known for its ability to effectively lyse cell membranes, making it a vital component in various research applications.
How does CTAB function as a lysis buffer?
CTAB acts as a detergent, disrupting cell membranes by interacting with lipids and proteins. This disruption allows for the release of cellular contents, such as DNA, RNA, and proteins, for downstream analysis.
What are the advantages of using CTAB as a lysis buffer?
CTAB offers several advantages as a lysis buffer, including its ability to effectively lyse a wide range of cell types, its compatibility with a variety of downstream applications, and its stability over a wide range of pH and temperature conditions. Additionally, CTAB is cost-effective and easy to use, making it a popular choice for researchers.
In what research applications is CTAB commonly used?
CTAB is commonly used in molecular biology and biochemistry research applications, such as DNA and RNA extraction, protein isolation, and plant genomic DNA isolation. Its versatility and effectiveness in lysing various cell types make it a valuable tool for researchers working with biological samples.
How should CTAB be stored and handled for optimal results?
CTAB should be stored in a cool, dry place away from light and moisture to maintain its stability. It is important to handle CTAB with care, following proper safety protocols, as it can be irritating to the skin and respiratory system. Additionally, CTAB should be used in a well-ventilated area to minimize exposure to fumes.
CTAB, or cetyltrimethylammonium bromide, is a quaternary ammonium salt commonly used in molecular biology and biochemistry. It is known for its ability to effectively lyse cell membranes, making it a vital component in various research applications.
How does CTAB function as a lysis buffer?
CTAB acts as a detergent, disrupting cell membranes by interacting with lipids and proteins. This disruption allows for the release of cellular contents, such as DNA, RNA, and proteins, for downstream analysis.
What are the advantages of using CTAB as a lysis buffer?
CTAB offers several advantages as a lysis buffer, including its ability to effectively lyse a wide range of cell types, its compatibility with a variety of downstream applications, and its stability over a wide range of pH and temperature conditions. Additionally, CTAB is cost-effective and easy to use, making it a popular choice for researchers.
In what research applications is CTAB commonly used?
CTAB is commonly used in molecular biology and biochemistry research applications, such as DNA and RNA extraction, protein isolation, and plant genomic DNA isolation. Its versatility and effectiveness in lysing various cell types make it a valuable tool for researchers working with biological samples.
How should CTAB be stored and handled for optimal results?
CTAB should be stored in a cool, dry place away from light and moisture to maintain its stability. It is important to handle CTAB with care, following proper safety protocols, as it can be irritating to the skin and respiratory system. Additionally, CTAB should be used in a well-ventilated area to minimize exposure to fumes.
