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| Name | EDTA - Solution pH 8.0 (0.5 M) |
| Type | Molecular Biology Product |
| Concentration | 0.5 M |
| PH | 8.0 |
| Form | Solution |
| Application | Chelating agent for metal ions |
| Storage | Store at room temperature |
| Appearance | Clear colorless liquid |
| Stability | Stable for at least 2 years when stored properly |
| Solubility | Soluble in water |
| Usage | Commonly used in molecular biology experiments |
| Purity | High purity grade |
| CAS Number | 6381-92-6 |
| Molecular Formula | C10H16N2O8 |
| Molecular Weight | 292.24 g/mol |
FAQ
What is EDTA and why is it important in molecular biology?
EDTA, or ethylenediaminetetraacetic acid, is a chelating agent commonly used in molecular biology to bind and inactivate divalent metal ions, such as Mg2+, Ca2+, and Fe2+, which are essential for many enzymatic reactions. By sequestering these metal ions, EDTA helps to stabilize enzymes and prevent metal-catalyzed oxidation reactions, making it a crucial component in various molecular biology applications.
What is the recommended pH and concentration of EDTA solution for molecular biology applications?
The recommended pH for EDTA solution in molecular biology applications is typically around pH 8.0, as this is the pH at which EDTA is most effective in chelating metal ions. A concentration of 0.5 M EDTA is commonly used in molecular biology to achieve optimal metal ion binding and enzyme stabilization.
How is EDTA solution pH 8.0 (0.5 M) used in DNA and RNA extractions?
EDTA solution pH 8.0 (0.5 M) is often included in DNA and RNA extraction buffers to chelate divalent cations that can degrade nucleic acids. By inhibiting metal-dependent nucleases that can cleave DNA and RNA molecules, EDTA helps to preserve the integrity of the nucleic acids during the extraction process, ensuring high-quality DNA and RNA yields.
What are some other applications of EDTA solution pH 8.0 (0.5 M) in molecular biology?
In addition to DNA and RNA extractions, EDTA solution pH 8.0 (0.5 M) is used in a variety of molecular biology applications, including PCR, DNA sequencing, restriction enzyme digestion, and protein isolation. By chelating metal ions that can interfere with enzymatic reactions or degrade biomolecules, EDTA helps to optimize experimental conditions and ensure reliable results.
How should EDTA solution pH 8.0 (0.5 M) be stored to maintain its stability and efficacy?
To maintain the stability and efficacy of EDTA solution pH 8.0 (0.5 M), it should be stored at room temperature away from light and moisture. It is important to use EDTA solution made with high-quality reagents and to periodically check its pH and concentration to ensure consistent performance in molecular biology experiments.
EDTA, or ethylenediaminetetraacetic acid, is a chelating agent commonly used in molecular biology to bind and inactivate divalent metal ions, such as Mg2+, Ca2+, and Fe2+, which are essential for many enzymatic reactions. By sequestering these metal ions, EDTA helps to stabilize enzymes and prevent metal-catalyzed oxidation reactions, making it a crucial component in various molecular biology applications.
What is the recommended pH and concentration of EDTA solution for molecular biology applications?
The recommended pH for EDTA solution in molecular biology applications is typically around pH 8.0, as this is the pH at which EDTA is most effective in chelating metal ions. A concentration of 0.5 M EDTA is commonly used in molecular biology to achieve optimal metal ion binding and enzyme stabilization.
How is EDTA solution pH 8.0 (0.5 M) used in DNA and RNA extractions?
EDTA solution pH 8.0 (0.5 M) is often included in DNA and RNA extraction buffers to chelate divalent cations that can degrade nucleic acids. By inhibiting metal-dependent nucleases that can cleave DNA and RNA molecules, EDTA helps to preserve the integrity of the nucleic acids during the extraction process, ensuring high-quality DNA and RNA yields.
What are some other applications of EDTA solution pH 8.0 (0.5 M) in molecular biology?
In addition to DNA and RNA extractions, EDTA solution pH 8.0 (0.5 M) is used in a variety of molecular biology applications, including PCR, DNA sequencing, restriction enzyme digestion, and protein isolation. By chelating metal ions that can interfere with enzymatic reactions or degrade biomolecules, EDTA helps to optimize experimental conditions and ensure reliable results.
How should EDTA solution pH 8.0 (0.5 M) be stored to maintain its stability and efficacy?
To maintain the stability and efficacy of EDTA solution pH 8.0 (0.5 M), it should be stored at room temperature away from light and moisture. It is important to use EDTA solution made with high-quality reagents and to periodically check its pH and concentration to ensure consistent performance in molecular biology experiments.
