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| Name | HEPES buffer pH 7.5 (1 M) sterile |
| Concentration | 1 M |
| PH | 7.5 |
| Formulation | Sterile solution |
| Volume | Varies |
| Storage Conditions | Store at room temperature |
| Stability | Stable for at least 1 year |
| Sterilization Method | Sterile filtered |
| Purity | High purity |
| Applications | N/A |
| Shipping Restrictions | Ships at ambient temperature |
| Packaging | Multiple sizes available |
| Manufacturer | Various manufacturers |
| Catalog Number | Varies by supplier |
| Shelf Life | Long shelf life |
| Availability | Commercially available |
| Solubility | Soluble in water |
| Compatibility | Compatible with most biological assays |
| Recommended Usage | Follow manufacturer's instructions |
| Handling Precautions | Use proper laboratory attire and techniques |
| Laboratory Use | N/A |
FAQ
What is HEPES buffer pH 7.5 (1 M) sterile?
HEPES buffer pH 7.5 (1 M) sterile is a ready-to-use buffer solution containing HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) at a concentration of 1 M. It is sterilized to ensure purity and is adjusted to a pH of 7.5, making it ideal for a wide range of biochemical and biological applications.
What are the benefits of using HEPES buffer pH 7.5 (1 M) sterile?
HEPES buffer pH 7.5 (1 M) sterile offers several advantages, including:
- Ready-to-use convenience: The buffer is pre-made and sterilized, saving time and effort in preparation.
- Stable pH: The pH of the buffer is carefully adjusted to 7.5 and remains stable over time, ensuring reliable results.
- Versatility: HEPES buffer is commonly used in a variety of biological and biochemical experiments, including cell culture, protein assays, and enzyme studies.
- High solubility: HEPES is highly soluble in water, making it easy to prepare desired concentrations for different applications.
- Sterility: The buffer is sterilized to minimize the risk of contamination, crucial for sensitive experiments.
How can I use HEPES buffer pH 7.5 (1 M) sterile in my experiments?
HEPES buffer pH 7.5 (1 M) sterile can be used in various applications requiring a buffering agent at pH 7.5. Some common uses include:
- Maintaining stable pH in cell culture media
- Diluting and storing proteins and enzymes
- Adjusting the pH of biochemical assays and reactions
- Stabilizing pH in electrophoresis buffers
- Resuspending cells or tissues for analysis
Simply add the appropriate volume of HEPES buffer to your experimental system to achieve the desired pH and buffering capacity.
Is HEPES buffer pH 7.5 (1 M) sterile compatible with my experimental system?
HEPES buffer pH 7.5 (1 M) sterile is compatible with most biological and biochemical systems. However, it is important to consider the specific requirements of your experiment and consult literature or technical resources to ensure compatibility. In general, HEPES buffer is widely used in cell culture, molecular biology, and protein biochemistry applications due to its stability and low cytotoxicity.
How should I store HEPES buffer pH 7.5 (1 M) sterile?
HEPES buffer pH 7.5 (1 M) sterile should be stored at room temperature and protected from light to maintain its stability. Avoid repeated freeze-thaw cycles, as this can compromise the quality of the buffer. It is recommended to aliquot the buffer into smaller volumes before freezing to prevent contamination and ensure convenient use.
HEPES buffer pH 7.5 (1 M) sterile is a ready-to-use buffer solution containing HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) at a concentration of 1 M. It is sterilized to ensure purity and is adjusted to a pH of 7.5, making it ideal for a wide range of biochemical and biological applications.
What are the benefits of using HEPES buffer pH 7.5 (1 M) sterile?
HEPES buffer pH 7.5 (1 M) sterile offers several advantages, including:
- Ready-to-use convenience: The buffer is pre-made and sterilized, saving time and effort in preparation.
- Stable pH: The pH of the buffer is carefully adjusted to 7.5 and remains stable over time, ensuring reliable results.
- Versatility: HEPES buffer is commonly used in a variety of biological and biochemical experiments, including cell culture, protein assays, and enzyme studies.
- High solubility: HEPES is highly soluble in water, making it easy to prepare desired concentrations for different applications.
- Sterility: The buffer is sterilized to minimize the risk of contamination, crucial for sensitive experiments.
How can I use HEPES buffer pH 7.5 (1 M) sterile in my experiments?
HEPES buffer pH 7.5 (1 M) sterile can be used in various applications requiring a buffering agent at pH 7.5. Some common uses include:
- Maintaining stable pH in cell culture media
- Diluting and storing proteins and enzymes
- Adjusting the pH of biochemical assays and reactions
- Stabilizing pH in electrophoresis buffers
- Resuspending cells or tissues for analysis
Simply add the appropriate volume of HEPES buffer to your experimental system to achieve the desired pH and buffering capacity.
Is HEPES buffer pH 7.5 (1 M) sterile compatible with my experimental system?
HEPES buffer pH 7.5 (1 M) sterile is compatible with most biological and biochemical systems. However, it is important to consider the specific requirements of your experiment and consult literature or technical resources to ensure compatibility. In general, HEPES buffer is widely used in cell culture, molecular biology, and protein biochemistry applications due to its stability and low cytotoxicity.
How should I store HEPES buffer pH 7.5 (1 M) sterile?
HEPES buffer pH 7.5 (1 M) sterile should be stored at room temperature and protected from light to maintain its stability. Avoid repeated freeze-thaw cycles, as this can compromise the quality of the buffer. It is recommended to aliquot the buffer into smaller volumes before freezing to prevent contamination and ensure convenient use.
