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Product Name | HEPES buffer pH 8.0 (1 M) sterile |
PH Level | 8 |
Concentration | 1 M |
Sterility | sterile |
Buffer Type | HEPES |
Volume | unknown |
Storage Conditions | Store at room temperature |
Packaging | Bottled |
Composition | HEPES, water |
Application | Used in biological and biochemical research |
Solubility | Soluble in water |
Purity Level | High purity grade |
Shelf Life | 1-2 years |
Manufacturer | Various |
Catalog Number | Depends on manufacturer |
FAQ
What is HEPES buffer pH 8.0 (1 M) sterile?
HEPES buffer pH 8.0 (1 M) sterile is a high-quality buffer solution used in biological and biochemical research. It is prepared with HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) to maintain a stable pH of 8.0 in cell culture media and biological assays. The buffer is sterile, ensuring that it is free from any contaminants that could interfere with experimental results.
What are the advantages of using HEPES buffer pH 8.0 (1 M) sterile?
HEPES buffer pH 8.0 (1 M) sterile offers several advantages for researchers. It provides a stable pH of 8.0, which is ideal for maintaining cell viability and enzyme activity in biological samples. The buffer is also sterile, eliminating the need for additional filtration or sterilization steps. Additionally, HEPES buffer is known for its low toxicity and compatibility with a wide range of biological systems.
How should HEPES buffer pH 8.0 (1 M) sterile be stored?
HEPES buffer pH 8.0 (1 M) sterile should be stored at room temperature away from direct sunlight and moisture. It is recommended to aliquot the buffer into smaller volumes to avoid repeated freeze-thaw cycles, which could affect its performance. Proper storage of the buffer will help maintain its stability and ensure consistent results in experiments.
How is HEPES buffer pH 8.0 (1 M) sterile used in cell culture?
HEPES buffer pH 8.0 (1 M) sterile is commonly used in cell culture to maintain a stable pH environment for cells. Researchers can add the buffer to their cell culture media to keep the pH at 8.0, which is optimal for many cell lines. The sterile nature of the buffer ensures that the cells are not exposed to any contaminants that could affect their growth and viability.
Can HEPES buffer pH 8.0 (1 M) sterile be used in other applications besides cell culture?
Yes, HEPES buffer pH 8.0 (1 M) sterile can be used in a variety of biological and biochemical applications. It is commonly employed in enzyme assays, protein purification, and molecular biology techniques that require a stable pH environment. The sterile formulation of the buffer makes it suitable for use in sensitive experiments where contamination could compromise results.
HEPES buffer pH 8.0 (1 M) sterile is a high-quality buffer solution used in biological and biochemical research. It is prepared with HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) to maintain a stable pH of 8.0 in cell culture media and biological assays. The buffer is sterile, ensuring that it is free from any contaminants that could interfere with experimental results.
What are the advantages of using HEPES buffer pH 8.0 (1 M) sterile?
HEPES buffer pH 8.0 (1 M) sterile offers several advantages for researchers. It provides a stable pH of 8.0, which is ideal for maintaining cell viability and enzyme activity in biological samples. The buffer is also sterile, eliminating the need for additional filtration or sterilization steps. Additionally, HEPES buffer is known for its low toxicity and compatibility with a wide range of biological systems.
How should HEPES buffer pH 8.0 (1 M) sterile be stored?
HEPES buffer pH 8.0 (1 M) sterile should be stored at room temperature away from direct sunlight and moisture. It is recommended to aliquot the buffer into smaller volumes to avoid repeated freeze-thaw cycles, which could affect its performance. Proper storage of the buffer will help maintain its stability and ensure consistent results in experiments.
How is HEPES buffer pH 8.0 (1 M) sterile used in cell culture?
HEPES buffer pH 8.0 (1 M) sterile is commonly used in cell culture to maintain a stable pH environment for cells. Researchers can add the buffer to their cell culture media to keep the pH at 8.0, which is optimal for many cell lines. The sterile nature of the buffer ensures that the cells are not exposed to any contaminants that could affect their growth and viability.
Can HEPES buffer pH 8.0 (1 M) sterile be used in other applications besides cell culture?
Yes, HEPES buffer pH 8.0 (1 M) sterile can be used in a variety of biological and biochemical applications. It is commonly employed in enzyme assays, protein purification, and molecular biology techniques that require a stable pH environment. The sterile formulation of the buffer makes it suitable for use in sensitive experiments where contamination could compromise results.