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| Product Name | HEPES for Cell Culture |
| Brand | Thermo Fisher Scientific |
| Packaging | Powder form |
| Buffering Agent | HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) |
| PH Range | 7.2 - 7.6 |
| Concentration | 1M |
| Sterility | Sterile-filtered |
| Storage Conditions | Store at room temperature |
| Usage | Cell culture media supplement |
| Compatibility | Compatible with most cell culture systems |
| Application | Maintaining constant pH in cell culture systems |
| Shelf Life | 2 years |
| Appearance | White crystalline powder |
| CAS Number | 7365-45-9 |
| EC Number | 230-907-9 |
| UNSPSC | 12354001 |
FAQ
What is HEPES and how is it used in cell culture?
HEPES, or N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, is a buffering agent commonly used in cell culture media. It helps to maintain the pH of the cell culture media, which is critical for cell growth and survival. HEPES is particularly useful in cell culture systems that do not have a CO2 incubator, as it can help to stabilize the pH in these conditions.
How does HEPES differ from other buffering agents used in cell culture?
HEPES is preferred over other buffering agents such as bicarbonate or phosphate buffers in cell culture for several reasons. Unlike bicarbonate, HEPES does not require a CO2 incubator for buffering and can be used in systems without CO2 control. This makes it more versatile for different cell culture setups. Additionally, HEPES has a buffering range of pH 6.8-8.2, which is ideal for maintaining physiological pH in cell culture media.
Can HEPES be toxic to cells in culture?
HEPES is generally considered to be non-toxic to cells in culture at the concentrations typically used in media (5-25 mM). However, it is important to optimize the concentration of HEPES in your cell culture system to ensure compatibility with your specific cell lines. It is always recommended to perform a pilot experiment to determine the effects of HEPES on cell viability and growth in your particular setup.
How should HEPES be stored and handled in the laboratory?
HEPES should be stored in a cool, dry place away from direct light. It is important to keep the container tightly closed when not in use to prevent contamination. When handling HEPES, it is recommended to wear appropriate personal protective equipment, such as gloves and safety goggles, to avoid skin and eye contact. Always follow the manufacturer's guidelines for proper storage and handling of HEPES in the laboratory.
Are there any specific cell culture applications where HEPES is particularly beneficial?
HEPES is widely used in a variety of cell culture applications, including primary cell culture, stem cell culture, and suspension cell culture. It is especially beneficial in systems where precise pH control is required, such as long-term culture experiments or sensitive cell lines. HEPES can also be used in specialized cell culture media formulations, such as serum-free or low-serum media, to help maintain optimal pH levels for cell growth and function.
HEPES, or N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, is a buffering agent commonly used in cell culture media. It helps to maintain the pH of the cell culture media, which is critical for cell growth and survival. HEPES is particularly useful in cell culture systems that do not have a CO2 incubator, as it can help to stabilize the pH in these conditions.
How does HEPES differ from other buffering agents used in cell culture?
HEPES is preferred over other buffering agents such as bicarbonate or phosphate buffers in cell culture for several reasons. Unlike bicarbonate, HEPES does not require a CO2 incubator for buffering and can be used in systems without CO2 control. This makes it more versatile for different cell culture setups. Additionally, HEPES has a buffering range of pH 6.8-8.2, which is ideal for maintaining physiological pH in cell culture media.
Can HEPES be toxic to cells in culture?
HEPES is generally considered to be non-toxic to cells in culture at the concentrations typically used in media (5-25 mM). However, it is important to optimize the concentration of HEPES in your cell culture system to ensure compatibility with your specific cell lines. It is always recommended to perform a pilot experiment to determine the effects of HEPES on cell viability and growth in your particular setup.
How should HEPES be stored and handled in the laboratory?
HEPES should be stored in a cool, dry place away from direct light. It is important to keep the container tightly closed when not in use to prevent contamination. When handling HEPES, it is recommended to wear appropriate personal protective equipment, such as gloves and safety goggles, to avoid skin and eye contact. Always follow the manufacturer's guidelines for proper storage and handling of HEPES in the laboratory.
Are there any specific cell culture applications where HEPES is particularly beneficial?
HEPES is widely used in a variety of cell culture applications, including primary cell culture, stem cell culture, and suspension cell culture. It is especially beneficial in systems where precise pH control is required, such as long-term culture experiments or sensitive cell lines. HEPES can also be used in specialized cell culture media formulations, such as serum-free or low-serum media, to help maintain optimal pH levels for cell growth and function.
