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| Product Name | PBS buffer (1X, Dulbecco's) - Powder |
| Buffer Type | Phosphate Buffered Saline |
| Concentration | 1X |
| Form | Powder |
| Package Size | 100 g |
| Storage Condition | Store at room temperature |
| Shelf Life | 2 years |
| Usage | For preparing cell culture media |
| PH Range | 7.2-7.6 |
| Ionic Strength | 0.154 M |
| Ingredients | Sodium chloride, Sodium phosphate, Potassium chloride, Potassium phosphate |
| Appearance | White crystalline powder |
FAQ
What is PBS buffer (1X, Dulbecco's) used for?
PBS buffer (1X, Dulbecco's) is commonly used in biological and biochemical research for various applications such as cell culture, protein assays, and immunohistochemistry. It helps maintain the pH and osmolarity of the solution, making it an ideal choice for washing, diluting, or storing biological samples.
How do I prepare PBS buffer (1X, Dulbecco's) from the powder form?
To prepare 1X PBS buffer from the powder form, simply dissolve the appropriate amount of powder in distilled water to achieve the desired concentration. Stir the solution gently until the powder is completely dissolved. Adjust the pH if necessary and sterilize the solution by filtering it through a 0.22 µm filter.
Can PBS buffer (1X, Dulbecco's) be used in cell culture applications?
Yes, PBS buffer (1X, Dulbecco's) is commonly used in cell culture applications for tasks such as washing cells, diluting samples, or reconstituting reagents. Its balanced salt composition and physiological pH make it compatible with most cell lines and ensure minimal cell stress during handling.
What are the advantages of using PBS buffer (1X, Dulbecco's) over other buffers?
PBS buffer (1X, Dulbecco's) offers several advantages over other buffers, such as its ability to maintain the pH and osmolarity of the solution, its compatibility with a wide range of biological samples, and its ease of preparation from the powder form. Additionally, PBS buffer is known for its stability and low cytotoxicity, making it a popular choice for various research applications.
In what other research applications can PBS buffer (1X, Dulbecco's) be used?
Apart from cell culture and protein assays, PBS buffer (1X, Dulbecco's) can be used in a variety of research applications such as immunohistochemistry, Western blotting, and molecular biology techniques. Its versatility and compatibility with different biological samples make it a valuable tool in the laboratory for a wide range of experiments.
PBS buffer (1X, Dulbecco's) is commonly used in biological and biochemical research for various applications such as cell culture, protein assays, and immunohistochemistry. It helps maintain the pH and osmolarity of the solution, making it an ideal choice for washing, diluting, or storing biological samples.
How do I prepare PBS buffer (1X, Dulbecco's) from the powder form?
To prepare 1X PBS buffer from the powder form, simply dissolve the appropriate amount of powder in distilled water to achieve the desired concentration. Stir the solution gently until the powder is completely dissolved. Adjust the pH if necessary and sterilize the solution by filtering it through a 0.22 µm filter.
Can PBS buffer (1X, Dulbecco's) be used in cell culture applications?
Yes, PBS buffer (1X, Dulbecco's) is commonly used in cell culture applications for tasks such as washing cells, diluting samples, or reconstituting reagents. Its balanced salt composition and physiological pH make it compatible with most cell lines and ensure minimal cell stress during handling.
What are the advantages of using PBS buffer (1X, Dulbecco's) over other buffers?
PBS buffer (1X, Dulbecco's) offers several advantages over other buffers, such as its ability to maintain the pH and osmolarity of the solution, its compatibility with a wide range of biological samples, and its ease of preparation from the powder form. Additionally, PBS buffer is known for its stability and low cytotoxicity, making it a popular choice for various research applications.
In what other research applications can PBS buffer (1X, Dulbecco's) be used?
Apart from cell culture and protein assays, PBS buffer (1X, Dulbecco's) can be used in a variety of research applications such as immunohistochemistry, Western blotting, and molecular biology techniques. Its versatility and compatibility with different biological samples make it a valuable tool in the laboratory for a wide range of experiments.
