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| Product Name | TAE Buffer (10X) |
| Application | Molecular Biology |
| PH Range | 8.3-8.6 |
| Concentration | 10X (1X working concentration is achieved by diluting 1 part of 10X buffer with 9 parts of water) |
| Storage Conditions | Store at room temperature |
| Stability | Stable for up to 2 years when stored properly |
| Composition | Tris Base, Sodium Acetate, EDTA |
| Usage | DNA and RNA agarose gel electrophoresis |
| Compatibility | Compatible with nucleic acid electrophoresis techniques |
| Volume | Available in various sizes (e.g. 100 mL, 500 mL) |
| Safety Information | Harmful if swallowed, avoid contact with skin and eyes, wear appropriate protective clothing and gloves when handling |
| Shipping Restrictions | Ships at ambient temperature, may be subject to special shipping requirements in some regions |
| Manufacturer | Various molecular biology reagent suppliers |
| Catalog Number | Dependent on the supplier |
| Country Of Origin | Dependent on the manufacturer |
| Dilution Factor | 1:10 (For 1X working concentration) |
FAQ
What is TAE buffer (10X) and how is it used in molecular biology?
TAE buffer is a commonly used buffer solution in molecular biology for agarose gel electrophoresis. It is used to separate DNA fragments based on their size by applying an electric field that causes negatively charged DNA molecules to move towards the positive electrode.
What are the components of TAE buffer (10X)?
TAE buffer (10X) typically contains Tris base, acetic acid, and EDTA. The Tris base helps to maintain the pH of the solution, acetic acid provides conductivity, and EDTA helps to chelate metal ions that could interfere with the DNA molecules.
How is TAE buffer (10X) prepared for use in agarose gel electrophoresis?
To prepare TAE buffer (10X) for use in agarose gel electrophoresis, the user simply needs to dilute the 10X concentrated solution with distilled water to achieve the desired working concentration. Typically, a 1X TAE buffer is used for most applications in molecular biology.
What are some advantages of using TAE buffer (10X) in agarose gel electrophoresis?
TAE buffer is known for its ability to provide consistent and reliable results in DNA electrophoresis. It has a relatively high buffering capacity, which helps to maintain a stable pH during the electrophoresis process. Additionally, TAE buffer is cost-effective and easy to prepare, making it a popular choice for many researchers.
Are there any limitations or considerations when using TAE buffer (10X) in molecular biology experiments?
While TAE buffer is widely used in molecular biology, there are some limitations and considerations to keep in mind. For example, TAE buffer is not recommended for applications that require high-resolution separation of DNA fragments, as it can result in excessive smearing of bands. Additionally, TAE buffer is not ideal for use with RNA samples, as EDTA can degrade RNA molecules.
Overall, TAE buffer (10X) is a versatile and reliable buffer solution that is essential for agarose gel electrophoresis in molecular biology research. Its ease of use, cost-effectiveness, and consistent performance make it a valuable tool for scientists working with DNA samples.
TAE buffer is a commonly used buffer solution in molecular biology for agarose gel electrophoresis. It is used to separate DNA fragments based on their size by applying an electric field that causes negatively charged DNA molecules to move towards the positive electrode.
What are the components of TAE buffer (10X)?
TAE buffer (10X) typically contains Tris base, acetic acid, and EDTA. The Tris base helps to maintain the pH of the solution, acetic acid provides conductivity, and EDTA helps to chelate metal ions that could interfere with the DNA molecules.
How is TAE buffer (10X) prepared for use in agarose gel electrophoresis?
To prepare TAE buffer (10X) for use in agarose gel electrophoresis, the user simply needs to dilute the 10X concentrated solution with distilled water to achieve the desired working concentration. Typically, a 1X TAE buffer is used for most applications in molecular biology.
What are some advantages of using TAE buffer (10X) in agarose gel electrophoresis?
TAE buffer is known for its ability to provide consistent and reliable results in DNA electrophoresis. It has a relatively high buffering capacity, which helps to maintain a stable pH during the electrophoresis process. Additionally, TAE buffer is cost-effective and easy to prepare, making it a popular choice for many researchers.
Are there any limitations or considerations when using TAE buffer (10X) in molecular biology experiments?
While TAE buffer is widely used in molecular biology, there are some limitations and considerations to keep in mind. For example, TAE buffer is not recommended for applications that require high-resolution separation of DNA fragments, as it can result in excessive smearing of bands. Additionally, TAE buffer is not ideal for use with RNA samples, as EDTA can degrade RNA molecules.
Overall, TAE buffer (10X) is a versatile and reliable buffer solution that is essential for agarose gel electrophoresis in molecular biology research. Its ease of use, cost-effectiveness, and consistent performance make it a valuable tool for scientists working with DNA samples.
