Back
| Name | TAE buffer (50X) |
| Application | Molecular biology |
| Purpose | Electrophoresis buffer for nucleic acid separation |
| Composition | Tris base, acetic acid, EDTA |
| Concentration | 50X |
| PH Range | 8.0 - 8.4 |
| Storage Conditions | Store at room temperature |
| Shelf Life | 2 years |
| Compatibility | RNA and DNA electrophoresis |
| Packaging | Available in various volumes |
| Preparation | Dilute 1 part of 50X TAE buffer with 49 parts of water to make a 1X working solution |
| Alternative Names | Tris-Acetate-EDTA buffer |
FAQ
What is the concentration of TAE buffer in the 50X solution?
The TAE buffer in this 50X solution is concentrated to provide a final concentration of 1X when diluted accordingly.
How is TAE buffer used in molecular biology applications?
TAE buffer is commonly used as a running buffer for agarose gel electrophoresis in molecular biology applications. It helps to provide the necessary pH environment and conductivity for the separation of DNA fragments based on size.
What are the advantages of using a 50X TAE buffer solution?
The 50X TAE buffer solution offers the convenience of a more concentrated stock solution, which can be easily diluted to the desired working concentration without the need for additional preparation steps. This can save time and ensure consistent results in your experiments.
Can TAE buffer be used in other laboratory techniques besides gel electrophoresis?
Yes, TAE buffer can also be used in other laboratory techniques such as nucleic acid purification, PCR, and DNA sequencing. It is a versatile buffer solution that is commonly used in various molecular biology protocols.
Is there any special storage requirement for the 50X TAE buffer solution?
It is recommended to store the 50X TAE buffer solution at room temperature and protected from light to maintain its stability. Make sure to properly seal the container after each use to prevent contamination and evaporation.
The TAE buffer in this 50X solution is concentrated to provide a final concentration of 1X when diluted accordingly.
How is TAE buffer used in molecular biology applications?
TAE buffer is commonly used as a running buffer for agarose gel electrophoresis in molecular biology applications. It helps to provide the necessary pH environment and conductivity for the separation of DNA fragments based on size.
What are the advantages of using a 50X TAE buffer solution?
The 50X TAE buffer solution offers the convenience of a more concentrated stock solution, which can be easily diluted to the desired working concentration without the need for additional preparation steps. This can save time and ensure consistent results in your experiments.
Can TAE buffer be used in other laboratory techniques besides gel electrophoresis?
Yes, TAE buffer can also be used in other laboratory techniques such as nucleic acid purification, PCR, and DNA sequencing. It is a versatile buffer solution that is commonly used in various molecular biology protocols.
Is there any special storage requirement for the 50X TAE buffer solution?
It is recommended to store the 50X TAE buffer solution at room temperature and protected from light to maintain its stability. Make sure to properly seal the container after each use to prevent contamination and evaporation.
