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| Name | TBE buffer (5X) |
| Product Type | Molecular biology reagent |
| Concentration | 5X |
| Usage | Electrophoresis |
| Size | 1L |
| Storage Conditions | Room temperature |
| Components | Tris Base, Boric Acid, EDTA |
| PH | 8.3 |
| Compatibility | Agarose gel electrophoresis |
| Purpose | Used for nucleic acid electrophoresis |
| Feature | Provides excellent resolution |
| Sterilized | Not sterilized |
| Shelf Life | 2 years |
| Manufacturing Company | Not specified |
| Availability | Commercially available |
| Shipping Restrictions | May require special shipping conditions |
| Recommended Dilution | Dilute 1:5 with water before use |
FAQ
What is TBE buffer used for in molecular biology?
TBE buffer is commonly used in molecular biology techniques such as agarose gel electrophoresis and polyacrylamide gel electrophoresis. It helps to provide the necessary ionic environment for nucleic acids to migrate through the gel during electrophoresis.
How is TBE buffer different from other types of electrophoresis buffers?
TBE buffer is specifically formulated for DNA and RNA electrophoresis, providing stable pH and conductivity levels ideal for separating nucleic acids. It is a concentrated solution that can be diluted to the desired working concentration, making it convenient and cost-effective for research labs.
What is the significance of using a 5X concentration of TBE buffer?
A 5X concentration of TBE buffer allows for easy dilution to the standard 1X working concentration used in most molecular biology applications. This saves time and resources by eliminating the need to prepare TBE buffer from scratch for each experiment.
Can TBE buffer be reused for multiple electrophoresis runs?
TBE buffer can be reused multiple times for electrophoresis runs as long as it remains uncontaminated and the pH and conductivity levels are within the acceptable range. However, it is recommended to replenish the buffer periodically to ensure consistent and reliable results.
Why is TBE buffer preferred over other types of electrophoresis buffers in molecular biology research?
TBE buffer is preferred over other types of electrophoresis buffers due to its stability, compatibility with a wide range of nucleic acid samples, and ease of use. Its ability to maintain a constant pH and conductivity during electrophoresis ensures accurate and reproducible results in molecular biology experiments.
TBE buffer is commonly used in molecular biology techniques such as agarose gel electrophoresis and polyacrylamide gel electrophoresis. It helps to provide the necessary ionic environment for nucleic acids to migrate through the gel during electrophoresis.
How is TBE buffer different from other types of electrophoresis buffers?
TBE buffer is specifically formulated for DNA and RNA electrophoresis, providing stable pH and conductivity levels ideal for separating nucleic acids. It is a concentrated solution that can be diluted to the desired working concentration, making it convenient and cost-effective for research labs.
What is the significance of using a 5X concentration of TBE buffer?
A 5X concentration of TBE buffer allows for easy dilution to the standard 1X working concentration used in most molecular biology applications. This saves time and resources by eliminating the need to prepare TBE buffer from scratch for each experiment.
Can TBE buffer be reused for multiple electrophoresis runs?
TBE buffer can be reused multiple times for electrophoresis runs as long as it remains uncontaminated and the pH and conductivity levels are within the acceptable range. However, it is recommended to replenish the buffer periodically to ensure consistent and reliable results.
Why is TBE buffer preferred over other types of electrophoresis buffers in molecular biology research?
TBE buffer is preferred over other types of electrophoresis buffers due to its stability, compatibility with a wide range of nucleic acid samples, and ease of use. Its ability to maintain a constant pH and conductivity during electrophoresis ensures accurate and reproducible results in molecular biology experiments.
